Cargando…
Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula
Most legumes can engage in symbiosis with N-fixing bacteria called rhizobia. This symbiosis, called nodulation, evolved from the more widespread symbiosis that most land plants form with arbuscular mycorrhiza, which is reflected in a common requirement of certain genes for both these symbioses. One...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020461/ https://www.ncbi.nlm.nih.gov/pubmed/31935845 http://dx.doi.org/10.3390/plants9010071 |
_version_ | 1783497750445293568 |
---|---|
author | Kumar, Anil Cousins, Donna R. Liu, Cheng-Wu Xu, Ping Murray, Jeremy D. |
author_facet | Kumar, Anil Cousins, Donna R. Liu, Cheng-Wu Xu, Ping Murray, Jeremy D. |
author_sort | Kumar, Anil |
collection | PubMed |
description | Most legumes can engage in symbiosis with N-fixing bacteria called rhizobia. This symbiosis, called nodulation, evolved from the more widespread symbiosis that most land plants form with arbuscular mycorrhiza, which is reflected in a common requirement of certain genes for both these symbioses. One key nodulation gene, Nodule Inception (NIN), has been intensively studied. Mutants in NIN are unable to form nodules, which has made it difficult to identify downstream genes under the control of NIN. The analysis of data from our recent transcriptomics study revealed that some genes with an altered expression of nin during nodulation are upregulated in mycorrhizal roots. In addition, another study reported the decreased colonization of nin roots by arbuscular mycorrhiza. We therefore investigated a role for NIN in mycorrhiza formation. Our time course study, using two nin alleles with differing genetic backgrounds, suggests that that loss of NIN does not affect colonization of Medicago truncatula roots, either in the presence or absence of rhizobia. This, and recent phylogenetic analyses showing that the loss of NIN is correlated with loss of nodulation in the FaFaCuRo clade, but not with the ability to form mycorrhiza, argue against NIN being required for arbuscular mycorrhization in legumes. |
format | Online Article Text |
id | pubmed-7020461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70204612020-03-09 Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula Kumar, Anil Cousins, Donna R. Liu, Cheng-Wu Xu, Ping Murray, Jeremy D. Plants (Basel) Brief Report Most legumes can engage in symbiosis with N-fixing bacteria called rhizobia. This symbiosis, called nodulation, evolved from the more widespread symbiosis that most land plants form with arbuscular mycorrhiza, which is reflected in a common requirement of certain genes for both these symbioses. One key nodulation gene, Nodule Inception (NIN), has been intensively studied. Mutants in NIN are unable to form nodules, which has made it difficult to identify downstream genes under the control of NIN. The analysis of data from our recent transcriptomics study revealed that some genes with an altered expression of nin during nodulation are upregulated in mycorrhizal roots. In addition, another study reported the decreased colonization of nin roots by arbuscular mycorrhiza. We therefore investigated a role for NIN in mycorrhiza formation. Our time course study, using two nin alleles with differing genetic backgrounds, suggests that that loss of NIN does not affect colonization of Medicago truncatula roots, either in the presence or absence of rhizobia. This, and recent phylogenetic analyses showing that the loss of NIN is correlated with loss of nodulation in the FaFaCuRo clade, but not with the ability to form mycorrhiza, argue against NIN being required for arbuscular mycorrhization in legumes. MDPI 2020-01-06 /pmc/articles/PMC7020461/ /pubmed/31935845 http://dx.doi.org/10.3390/plants9010071 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Kumar, Anil Cousins, Donna R. Liu, Cheng-Wu Xu, Ping Murray, Jeremy D. Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula |
title | Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula |
title_full | Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula |
title_fullStr | Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula |
title_full_unstemmed | Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula |
title_short | Nodule Inception Is Not Required for Arbuscular Mycorrhizal Colonization of Medicago truncatula |
title_sort | nodule inception is not required for arbuscular mycorrhizal colonization of medicago truncatula |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020461/ https://www.ncbi.nlm.nih.gov/pubmed/31935845 http://dx.doi.org/10.3390/plants9010071 |
work_keys_str_mv | AT kumaranil noduleinceptionisnotrequiredforarbuscularmycorrhizalcolonizationofmedicagotruncatula AT cousinsdonnar noduleinceptionisnotrequiredforarbuscularmycorrhizalcolonizationofmedicagotruncatula AT liuchengwu noduleinceptionisnotrequiredforarbuscularmycorrhizalcolonizationofmedicagotruncatula AT xuping noduleinceptionisnotrequiredforarbuscularmycorrhizalcolonizationofmedicagotruncatula AT murrayjeremyd noduleinceptionisnotrequiredforarbuscularmycorrhizalcolonizationofmedicagotruncatula |