Cargando…
Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice
SCOPE: As a prebiotic, inulin may have a protective effect on glucose metabolism. However, the mechanism of inulin treatment on glucose intolerance in offspring exposed to a maternal high-fat (HF) diet is still not clear. Here, we examined the hepatic DNA methylation profile to determine how materna...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020697/ https://www.ncbi.nlm.nih.gov/pubmed/32116778 http://dx.doi.org/10.3389/fphys.2020.00070 |
_version_ | 1783497792970293248 |
---|---|
author | Zhang, Qian Xiao, Xinhua Zheng, Jia Li, Ming Yu, Miao Ping, Fan Wang, Tong Wang, Xiaojing |
author_facet | Zhang, Qian Xiao, Xinhua Zheng, Jia Li, Ming Yu, Miao Ping, Fan Wang, Tong Wang, Xiaojing |
author_sort | Zhang, Qian |
collection | PubMed |
description | SCOPE: As a prebiotic, inulin may have a protective effect on glucose metabolism. However, the mechanism of inulin treatment on glucose intolerance in offspring exposed to a maternal high-fat (HF) diet is still not clear. Here, we examined the hepatic DNA methylation profile to determine how maternal inulin supplementation modified glucose metabolism in offspring mice. PROCEDURES: Female mice were fed a HF diet, control diet (CON), or a HF diet with inulin supplementation (HF-inulin) during gestation and lactation. Upon weaning, pup livers were obtained. A hepatic genome DNA methylation array was performed. RESULTS: Pups exposed to a maternal HF diet exhibited glucose intolerance and insulin resistance. Maternal inulin treatment moderated glucose metabolism. A DNA methylation array identified differentially methylated regions associated with 970 annotated genes from pups exposed to a HF diet in response to maternal inulin treatment. In particular, the wingless-type MMTV integration site family member 5A (Wnt5a) gene was hypermethylated, and the phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha (Pik3c2a), phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), and phosphoinositide-3-kinase regulatory subunit 2 (Pik3r2) genes were hypomethylated in inulin-treated pups. Consistently, hepatic Wnt5a gene expression was reduced and Pik3c2a, Pik3c2b, and Pik3r2 gene expression were increased in the inulin group. CONCLUSION: Maternal inulin treatment improved glucose intolerance by changing DNA methylation and gene expression of Wnt5a and Pi3k in mice exposed to a maternal HF diet. |
format | Online Article Text |
id | pubmed-7020697 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70206972020-02-28 Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice Zhang, Qian Xiao, Xinhua Zheng, Jia Li, Ming Yu, Miao Ping, Fan Wang, Tong Wang, Xiaojing Front Physiol Physiology SCOPE: As a prebiotic, inulin may have a protective effect on glucose metabolism. However, the mechanism of inulin treatment on glucose intolerance in offspring exposed to a maternal high-fat (HF) diet is still not clear. Here, we examined the hepatic DNA methylation profile to determine how maternal inulin supplementation modified glucose metabolism in offspring mice. PROCEDURES: Female mice were fed a HF diet, control diet (CON), or a HF diet with inulin supplementation (HF-inulin) during gestation and lactation. Upon weaning, pup livers were obtained. A hepatic genome DNA methylation array was performed. RESULTS: Pups exposed to a maternal HF diet exhibited glucose intolerance and insulin resistance. Maternal inulin treatment moderated glucose metabolism. A DNA methylation array identified differentially methylated regions associated with 970 annotated genes from pups exposed to a HF diet in response to maternal inulin treatment. In particular, the wingless-type MMTV integration site family member 5A (Wnt5a) gene was hypermethylated, and the phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha (Pik3c2a), phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta (Pik3c2b), and phosphoinositide-3-kinase regulatory subunit 2 (Pik3r2) genes were hypomethylated in inulin-treated pups. Consistently, hepatic Wnt5a gene expression was reduced and Pik3c2a, Pik3c2b, and Pik3r2 gene expression were increased in the inulin group. CONCLUSION: Maternal inulin treatment improved glucose intolerance by changing DNA methylation and gene expression of Wnt5a and Pi3k in mice exposed to a maternal HF diet. Frontiers Media S.A. 2020-02-07 /pmc/articles/PMC7020697/ /pubmed/32116778 http://dx.doi.org/10.3389/fphys.2020.00070 Text en Copyright © 2020 Zhang, Xiao, Zheng, Li, Yu, Ping, Wang and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Zhang, Qian Xiao, Xinhua Zheng, Jia Li, Ming Yu, Miao Ping, Fan Wang, Tong Wang, Xiaojing Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice |
title | Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice |
title_full | Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice |
title_fullStr | Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice |
title_full_unstemmed | Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice |
title_short | Maternal Inulin Supplementation Alters Hepatic DNA Methylation Profile and Improves Glucose Metabolism in Offspring Mice |
title_sort | maternal inulin supplementation alters hepatic dna methylation profile and improves glucose metabolism in offspring mice |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020697/ https://www.ncbi.nlm.nih.gov/pubmed/32116778 http://dx.doi.org/10.3389/fphys.2020.00070 |
work_keys_str_mv | AT zhangqian maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice AT xiaoxinhua maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice AT zhengjia maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice AT liming maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice AT yumiao maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice AT pingfan maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice AT wangtong maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice AT wangxiaojing maternalinulinsupplementationaltershepaticdnamethylationprofileandimprovesglucosemetabolisminoffspringmice |