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Optimization of Secreted Recombinant Human Epidermal Growth Factor Production Using Pectate Lyase B from Escherichia Coli BL21(DE3) by Central Composite Design and Its Production in High Cell Density Culture

CONTEXT: Human Epidermal Growth Factor (hEGF) is a potential therapeutic protein that has been widely used as a healing agent for various chronic wounds. It induces the proliferation and metabolism of epithelial cells, regenerates skin cells, and validates skin elasticity. In the previous study, rec...

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Detalles Bibliográficos
Autores principales:	Sriwidodo, Sriwidodo, Subroto, Toto, Maksum, Iman P., Wathoni, Nasrul, Rostinawati, Tina, Ulya, Himmatul, Putri, Indah U.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Wolters Kluwer - Medknow 2019
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020833/ https://www.ncbi.nlm.nih.gov/pubmed/32148364 http://dx.doi.org/10.4103/jpbs.JPBS_207_19

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