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Single molecule sensing of amyloid-β aggregation by confined glass nanopores

We have developed a glass nanopore based single molecule tool to investigate the dynamic oligomerization and aggregation process of Aβ1–42 peptides. The intrinsic differences in the molecular size and surface charge of amyloid aggregated states could be distinguished through single molecule induced...

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Autores principales: Yu, Ru-Jia, Lu, Si-Min, Xu, Su-Wen, Li, Yuan-Jie, Xu, Qun, Ying, Yi-Lun, Long, Yi-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020925/
https://www.ncbi.nlm.nih.gov/pubmed/32153747
http://dx.doi.org/10.1039/c9sc03260f
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author Yu, Ru-Jia
Lu, Si-Min
Xu, Su-Wen
Li, Yuan-Jie
Xu, Qun
Ying, Yi-Lun
Long, Yi-Tao
author_facet Yu, Ru-Jia
Lu, Si-Min
Xu, Su-Wen
Li, Yuan-Jie
Xu, Qun
Ying, Yi-Lun
Long, Yi-Tao
author_sort Yu, Ru-Jia
collection PubMed
description We have developed a glass nanopore based single molecule tool to investigate the dynamic oligomerization and aggregation process of Aβ1–42 peptides. The intrinsic differences in the molecular size and surface charge of amyloid aggregated states could be distinguished through single molecule induced characteristic current fluctuation. More importantly, our results reveal that the neurotoxic Aβ1–42 oligomer tends to adsorb onto the solid surface of nanopores, which may explain its instability and highly neurotoxic features.
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spelling pubmed-70209252020-03-09 Single molecule sensing of amyloid-β aggregation by confined glass nanopores Yu, Ru-Jia Lu, Si-Min Xu, Su-Wen Li, Yuan-Jie Xu, Qun Ying, Yi-Lun Long, Yi-Tao Chem Sci Chemistry We have developed a glass nanopore based single molecule tool to investigate the dynamic oligomerization and aggregation process of Aβ1–42 peptides. The intrinsic differences in the molecular size and surface charge of amyloid aggregated states could be distinguished through single molecule induced characteristic current fluctuation. More importantly, our results reveal that the neurotoxic Aβ1–42 oligomer tends to adsorb onto the solid surface of nanopores, which may explain its instability and highly neurotoxic features. Royal Society of Chemistry 2019-10-08 /pmc/articles/PMC7020925/ /pubmed/32153747 http://dx.doi.org/10.1039/c9sc03260f Text en This journal is © The Royal Society of Chemistry 2019 http://creativecommons.org/licenses/by-nc/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution Non Commercial 3.0 Unported Licence (CC BY-NC 3.0)
spellingShingle Chemistry
Yu, Ru-Jia
Lu, Si-Min
Xu, Su-Wen
Li, Yuan-Jie
Xu, Qun
Ying, Yi-Lun
Long, Yi-Tao
Single molecule sensing of amyloid-β aggregation by confined glass nanopores
title Single molecule sensing of amyloid-β aggregation by confined glass nanopores
title_full Single molecule sensing of amyloid-β aggregation by confined glass nanopores
title_fullStr Single molecule sensing of amyloid-β aggregation by confined glass nanopores
title_full_unstemmed Single molecule sensing of amyloid-β aggregation by confined glass nanopores
title_short Single molecule sensing of amyloid-β aggregation by confined glass nanopores
title_sort single molecule sensing of amyloid-β aggregation by confined glass nanopores
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7020925/
https://www.ncbi.nlm.nih.gov/pubmed/32153747
http://dx.doi.org/10.1039/c9sc03260f
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