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Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis

Opisthopappus taihangensis (Ling) Shih, as a relative of chrysanthemum, mainly survives on the cracks of steep slopes and cliffs. Due to the harsh environment in which O. taihangensis lives, it has evolved strong adaptive traits to drought stress. The root system first perceives soil water deficienc...

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Autores principales: Yang, Yongjuan, Guo, Yanhong, Zhong, Jian, Zhang, Tengxun, Li, Dawei, Ba, Tingting, Xu, Ting, Chang, Lina, Zhang, Qixiang, Sun, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7021704/
https://www.ncbi.nlm.nih.gov/pubmed/32060321
http://dx.doi.org/10.1038/s41598-020-59399-0
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author Yang, Yongjuan
Guo, Yanhong
Zhong, Jian
Zhang, Tengxun
Li, Dawei
Ba, Tingting
Xu, Ting
Chang, Lina
Zhang, Qixiang
Sun, Ming
author_facet Yang, Yongjuan
Guo, Yanhong
Zhong, Jian
Zhang, Tengxun
Li, Dawei
Ba, Tingting
Xu, Ting
Chang, Lina
Zhang, Qixiang
Sun, Ming
author_sort Yang, Yongjuan
collection PubMed
description Opisthopappus taihangensis (Ling) Shih, as a relative of chrysanthemum, mainly survives on the cracks of steep slopes and cliffs. Due to the harsh environment in which O. taihangensis lives, it has evolved strong adaptive traits to drought stress. The root system first perceives soil water deficiency, triggering a multi-pronged response mechanism to maintain water potential; however, the drought tolerance mechanism of O. taihangensis roots remains unclear. Therefore, roots were selected as materials to explore the physiological and molecular responsive mechanisms. We found that the roots had a stronger water retention capacity than the leaves. This result was attributed to ABA accumulation, which promoted an increased accumulation of proline and trehalose to maintain cell osmotic pressure, activated SOD and POD to scavenge ROS to protect root cell membrane structure and induced suberin depositions to minimize water backflow to dry soil. Transcriptome sequencing analyses further confirmed that O. taihangensis strongly activated genes involved in the ABA signalling pathway, osmolyte metabolism, antioxidant enzyme activity and biosynthesis of suberin monomer. Overall, these results not only will provide new insights into the drought response mechanisms of O. taihangensis but also will be helpful for future drought breeding programmes of chrysanthemum.
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spelling pubmed-70217042020-02-24 Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis Yang, Yongjuan Guo, Yanhong Zhong, Jian Zhang, Tengxun Li, Dawei Ba, Tingting Xu, Ting Chang, Lina Zhang, Qixiang Sun, Ming Sci Rep Article Opisthopappus taihangensis (Ling) Shih, as a relative of chrysanthemum, mainly survives on the cracks of steep slopes and cliffs. Due to the harsh environment in which O. taihangensis lives, it has evolved strong adaptive traits to drought stress. The root system first perceives soil water deficiency, triggering a multi-pronged response mechanism to maintain water potential; however, the drought tolerance mechanism of O. taihangensis roots remains unclear. Therefore, roots were selected as materials to explore the physiological and molecular responsive mechanisms. We found that the roots had a stronger water retention capacity than the leaves. This result was attributed to ABA accumulation, which promoted an increased accumulation of proline and trehalose to maintain cell osmotic pressure, activated SOD and POD to scavenge ROS to protect root cell membrane structure and induced suberin depositions to minimize water backflow to dry soil. Transcriptome sequencing analyses further confirmed that O. taihangensis strongly activated genes involved in the ABA signalling pathway, osmolyte metabolism, antioxidant enzyme activity and biosynthesis of suberin monomer. Overall, these results not only will provide new insights into the drought response mechanisms of O. taihangensis but also will be helpful for future drought breeding programmes of chrysanthemum. Nature Publishing Group UK 2020-02-14 /pmc/articles/PMC7021704/ /pubmed/32060321 http://dx.doi.org/10.1038/s41598-020-59399-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Yongjuan
Guo, Yanhong
Zhong, Jian
Zhang, Tengxun
Li, Dawei
Ba, Tingting
Xu, Ting
Chang, Lina
Zhang, Qixiang
Sun, Ming
Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis
title Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis
title_full Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis
title_fullStr Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis
title_full_unstemmed Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis
title_short Root Physiological Traits and Transcriptome Analyses Reveal that Root Zone Water Retention Confers Drought Tolerance to Opisthopappus taihangensis
title_sort root physiological traits and transcriptome analyses reveal that root zone water retention confers drought tolerance to opisthopappus taihangensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7021704/
https://www.ncbi.nlm.nih.gov/pubmed/32060321
http://dx.doi.org/10.1038/s41598-020-59399-0
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