Cargando…

Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells

In recent decades, several spices have been studied for their potential in the prevention and treatment of cancer. It is documented that spices have antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects. The main mechanisms of spices action included apoptosis induction, proliferat...

Descripción completa

Detalles Bibliográficos
Autores principales: Kello, Martin, Takac, Peter, Kubatka, Peter, Kuruc, Tomas, Petrova, Klaudia, Mojzis, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022383/
https://www.ncbi.nlm.nih.gov/pubmed/31947708
http://dx.doi.org/10.3390/biom10010139
_version_ 1783498002938200064
author Kello, Martin
Takac, Peter
Kubatka, Peter
Kuruc, Tomas
Petrova, Klaudia
Mojzis, Jan
author_facet Kello, Martin
Takac, Peter
Kubatka, Peter
Kuruc, Tomas
Petrova, Klaudia
Mojzis, Jan
author_sort Kello, Martin
collection PubMed
description In recent decades, several spices have been studied for their potential in the prevention and treatment of cancer. It is documented that spices have antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects. The main mechanisms of spices action included apoptosis induction, proliferation, migration and invasion of tumour inhibition, and sensitization of tumours to radiotherapy and chemotherapy. In this study, the ability of clove buds extract (CBE) to induce oxidative stress, DNA damage, and stress/survival/apoptotic pathways modulation were analysed in MCF-7 cells. We demonstrated that CBE treatment induced intrinsic caspase-dependent cell death associated with increased oxidative stress mediated by oxygen and nitrogen radicals. We showed also the CBE-mediated release of mitochondrial pro-apoptotic factors, signalling of oxidative stress-mediated DNA damage with modulation of cell antioxidant SOD (superoxide dismutase) system, and modulation activity of the Akt, p38 MAPK, JNK and Erk 1/2 pathways.
format Online
Article
Text
id pubmed-7022383
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70223832020-03-09 Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells Kello, Martin Takac, Peter Kubatka, Peter Kuruc, Tomas Petrova, Klaudia Mojzis, Jan Biomolecules Article In recent decades, several spices have been studied for their potential in the prevention and treatment of cancer. It is documented that spices have antioxidant, anti-inflammatory, immunomodulatory, and anticancer effects. The main mechanisms of spices action included apoptosis induction, proliferation, migration and invasion of tumour inhibition, and sensitization of tumours to radiotherapy and chemotherapy. In this study, the ability of clove buds extract (CBE) to induce oxidative stress, DNA damage, and stress/survival/apoptotic pathways modulation were analysed in MCF-7 cells. We demonstrated that CBE treatment induced intrinsic caspase-dependent cell death associated with increased oxidative stress mediated by oxygen and nitrogen radicals. We showed also the CBE-mediated release of mitochondrial pro-apoptotic factors, signalling of oxidative stress-mediated DNA damage with modulation of cell antioxidant SOD (superoxide dismutase) system, and modulation activity of the Akt, p38 MAPK, JNK and Erk 1/2 pathways. MDPI 2020-01-14 /pmc/articles/PMC7022383/ /pubmed/31947708 http://dx.doi.org/10.3390/biom10010139 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kello, Martin
Takac, Peter
Kubatka, Peter
Kuruc, Tomas
Petrova, Klaudia
Mojzis, Jan
Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
title Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
title_full Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
title_fullStr Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
title_full_unstemmed Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
title_short Oxidative Stress-Induced DNA Damage and Apoptosis in Clove Buds-Treated MCF-7 Cells
title_sort oxidative stress-induced dna damage and apoptosis in clove buds-treated mcf-7 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022383/
https://www.ncbi.nlm.nih.gov/pubmed/31947708
http://dx.doi.org/10.3390/biom10010139
work_keys_str_mv AT kellomartin oxidativestressinduceddnadamageandapoptosisinclovebudstreatedmcf7cells
AT takacpeter oxidativestressinduceddnadamageandapoptosisinclovebudstreatedmcf7cells
AT kubatkapeter oxidativestressinduceddnadamageandapoptosisinclovebudstreatedmcf7cells
AT kuructomas oxidativestressinduceddnadamageandapoptosisinclovebudstreatedmcf7cells
AT petrovaklaudia oxidativestressinduceddnadamageandapoptosisinclovebudstreatedmcf7cells
AT mojzisjan oxidativestressinduceddnadamageandapoptosisinclovebudstreatedmcf7cells