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Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions

Nowadays, there is a large amount of research aimed at improving the multifunctional behavior of the biomaterials for bone contact, including the concomitant ability to induce apatite formation (bioactivity), fast and effective osteoblasts colonization, and antibacterial activity. The aim of this st...

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Autores principales: Cochis, Andrea, Barberi, Jacopo, Ferraris, Sara, Miola, Marta, Rimondini, Lia, Vernè, Enrica, Yamaguchi, Seiji, Spriano, Silvia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022475/
https://www.ncbi.nlm.nih.gov/pubmed/31936394
http://dx.doi.org/10.3390/nano10010120
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author Cochis, Andrea
Barberi, Jacopo
Ferraris, Sara
Miola, Marta
Rimondini, Lia
Vernè, Enrica
Yamaguchi, Seiji
Spriano, Silvia
author_facet Cochis, Andrea
Barberi, Jacopo
Ferraris, Sara
Miola, Marta
Rimondini, Lia
Vernè, Enrica
Yamaguchi, Seiji
Spriano, Silvia
author_sort Cochis, Andrea
collection PubMed
description Nowadays, there is a large amount of research aimed at improving the multifunctional behavior of the biomaterials for bone contact, including the concomitant ability to induce apatite formation (bioactivity), fast and effective osteoblasts colonization, and antibacterial activity. The aim of this study is to develop antibacterial and bioactive surfaces (Ti6Al4V alloy and a silica-based bioactive glass) by chemical doping with strontium and/or silver ions. The surfaces were characterized by Scanning Electron Microscopy equipped with Energy Dispersive X ray Spectroscopy (SEM-EDS), X-ray photoelectron spectroscopy (XPS), and Transmission Electron Microscopy (TEM). To better focus on the cells–bacteria competition for the implant surface, in addition to the standard assays for the evaluation of the bacteria adhesion (ISO22196) and for single-cell cultures or biofilm formation, an innovative set of co-cultures of cells and bacteria is here proposed to simulate a competitive surface colonization. The results suggest that all the bioactive tested materials were cytocompatible toward the bone progenitor cells representative for the self-healing process, and that the doped ones were effective in reducing the surface colonization from a pathogenic drug-resistant strain of Staphylococcus aureus. The co-cultures experiments demonstrated that the doped surfaces were able to protect the adhered osteoblasts from the bacteria colonization as well as prevent the infection prior to the surface colonization by the osteoblasts.
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spelling pubmed-70224752020-03-09 Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions Cochis, Andrea Barberi, Jacopo Ferraris, Sara Miola, Marta Rimondini, Lia Vernè, Enrica Yamaguchi, Seiji Spriano, Silvia Nanomaterials (Basel) Article Nowadays, there is a large amount of research aimed at improving the multifunctional behavior of the biomaterials for bone contact, including the concomitant ability to induce apatite formation (bioactivity), fast and effective osteoblasts colonization, and antibacterial activity. The aim of this study is to develop antibacterial and bioactive surfaces (Ti6Al4V alloy and a silica-based bioactive glass) by chemical doping with strontium and/or silver ions. The surfaces were characterized by Scanning Electron Microscopy equipped with Energy Dispersive X ray Spectroscopy (SEM-EDS), X-ray photoelectron spectroscopy (XPS), and Transmission Electron Microscopy (TEM). To better focus on the cells–bacteria competition for the implant surface, in addition to the standard assays for the evaluation of the bacteria adhesion (ISO22196) and for single-cell cultures or biofilm formation, an innovative set of co-cultures of cells and bacteria is here proposed to simulate a competitive surface colonization. The results suggest that all the bioactive tested materials were cytocompatible toward the bone progenitor cells representative for the self-healing process, and that the doped ones were effective in reducing the surface colonization from a pathogenic drug-resistant strain of Staphylococcus aureus. The co-cultures experiments demonstrated that the doped surfaces were able to protect the adhered osteoblasts from the bacteria colonization as well as prevent the infection prior to the surface colonization by the osteoblasts. MDPI 2020-01-08 /pmc/articles/PMC7022475/ /pubmed/31936394 http://dx.doi.org/10.3390/nano10010120 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cochis, Andrea
Barberi, Jacopo
Ferraris, Sara
Miola, Marta
Rimondini, Lia
Vernè, Enrica
Yamaguchi, Seiji
Spriano, Silvia
Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions
title Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions
title_full Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions
title_fullStr Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions
title_full_unstemmed Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions
title_short Competitive Surface Colonization of Antibacterial and Bioactive Materials Doped with Strontium and/or Silver Ions
title_sort competitive surface colonization of antibacterial and bioactive materials doped with strontium and/or silver ions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022475/
https://www.ncbi.nlm.nih.gov/pubmed/31936394
http://dx.doi.org/10.3390/nano10010120
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