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Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand

Flesh flies (Sarcophagidae) are necrophagous insects initially colonizing on a corpse. The species-specific developmental data of the flies collected from a death scene can be used to estimate the minimum postmortem interval (PMI(min)). Thus, the first crucial step is to correctly identify the fly s...

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Autores principales: Samerjai, Chutharat, Sukontason, Kabkaew L., Sontigun, Narin, Sukontason, Kom, Klong-klaew, Tunwadee, Chareonviriyaphap, Theeraphap, Kurahashi, Hiromu, Klimpel, Sven, Kochmann, Judith, Saeung, Atiporn, Somboon, Pradya, Wannasan, Anchalee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022518/
https://www.ncbi.nlm.nih.gov/pubmed/31861293
http://dx.doi.org/10.3390/insects11010002
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author Samerjai, Chutharat
Sukontason, Kabkaew L.
Sontigun, Narin
Sukontason, Kom
Klong-klaew, Tunwadee
Chareonviriyaphap, Theeraphap
Kurahashi, Hiromu
Klimpel, Sven
Kochmann, Judith
Saeung, Atiporn
Somboon, Pradya
Wannasan, Anchalee
author_facet Samerjai, Chutharat
Sukontason, Kabkaew L.
Sontigun, Narin
Sukontason, Kom
Klong-klaew, Tunwadee
Chareonviriyaphap, Theeraphap
Kurahashi, Hiromu
Klimpel, Sven
Kochmann, Judith
Saeung, Atiporn
Somboon, Pradya
Wannasan, Anchalee
author_sort Samerjai, Chutharat
collection PubMed
description Flesh flies (Sarcophagidae) are necrophagous insects initially colonizing on a corpse. The species-specific developmental data of the flies collected from a death scene can be used to estimate the minimum postmortem interval (PMI(min)). Thus, the first crucial step is to correctly identify the fly species. Because of the high similarity among species of flesh flies, DNA-based identification is considered more favorable than morphology-based identification. In this study, we demonstrated the effectiveness of combined sequences (2216 to 2218 bp) of cytochrome c oxidase subunit I and II genes (COI and COII) for identification of the following 14 forensically important flesh fly species in Thailand: Boettcherisca nathani Lopes, Fengia ostindicae (Senior-White), Harpagophalla kempi (Senior-White), Liopygia ruficornis (Fabricius), Lioproctia pattoni (Senior-White), Lioproctia saprianovae (Pape & Bänziger), Parasarcophaga albiceps (Meigen), Parasarcophaga brevicornis (Ho), Parasarcophaga dux (Thomson), Parasarcophaga misera (Walker), Sarcorohdendorfia antilope (Böttcher), Sarcorohdendorfia inextricata (Walker), Sarcorohdendorfia seniorwhitei (Ho) and Seniorwhitea princeps (Wiedemann). Nucleotide variations of Thai flesh flies were evenly distributed throughout the COI-COII genes. Mean intra- and interspecific variations ranged from 0.00 to 0.96% and 5.22% to 12.31%, respectively. Using Best Match (BM) and Best Close Match (BCM) criteria, identification success for the combined genes was 100%, while the All Species Barcodes (ASB) criterion showed 76.74% success. Maximum Likelihood (ML) and Bayesian Inference (BI) phylogenetic analyses yielded similar tree topologies of monophyletic clades between species with very strong support values. The achieved sequences covering 14 forensically important flesh fly species including newly submitted sequences for B. nathani, F. ostindicae and S. seniorwhitei, can serve as a reliable reference database for further forensic entomological research in Thailand and in other areas where those species occur.
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spelling pubmed-70225182020-03-09 Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand Samerjai, Chutharat Sukontason, Kabkaew L. Sontigun, Narin Sukontason, Kom Klong-klaew, Tunwadee Chareonviriyaphap, Theeraphap Kurahashi, Hiromu Klimpel, Sven Kochmann, Judith Saeung, Atiporn Somboon, Pradya Wannasan, Anchalee Insects Article Flesh flies (Sarcophagidae) are necrophagous insects initially colonizing on a corpse. The species-specific developmental data of the flies collected from a death scene can be used to estimate the minimum postmortem interval (PMI(min)). Thus, the first crucial step is to correctly identify the fly species. Because of the high similarity among species of flesh flies, DNA-based identification is considered more favorable than morphology-based identification. In this study, we demonstrated the effectiveness of combined sequences (2216 to 2218 bp) of cytochrome c oxidase subunit I and II genes (COI and COII) for identification of the following 14 forensically important flesh fly species in Thailand: Boettcherisca nathani Lopes, Fengia ostindicae (Senior-White), Harpagophalla kempi (Senior-White), Liopygia ruficornis (Fabricius), Lioproctia pattoni (Senior-White), Lioproctia saprianovae (Pape & Bänziger), Parasarcophaga albiceps (Meigen), Parasarcophaga brevicornis (Ho), Parasarcophaga dux (Thomson), Parasarcophaga misera (Walker), Sarcorohdendorfia antilope (Böttcher), Sarcorohdendorfia inextricata (Walker), Sarcorohdendorfia seniorwhitei (Ho) and Seniorwhitea princeps (Wiedemann). Nucleotide variations of Thai flesh flies were evenly distributed throughout the COI-COII genes. Mean intra- and interspecific variations ranged from 0.00 to 0.96% and 5.22% to 12.31%, respectively. Using Best Match (BM) and Best Close Match (BCM) criteria, identification success for the combined genes was 100%, while the All Species Barcodes (ASB) criterion showed 76.74% success. Maximum Likelihood (ML) and Bayesian Inference (BI) phylogenetic analyses yielded similar tree topologies of monophyletic clades between species with very strong support values. The achieved sequences covering 14 forensically important flesh fly species including newly submitted sequences for B. nathani, F. ostindicae and S. seniorwhitei, can serve as a reliable reference database for further forensic entomological research in Thailand and in other areas where those species occur. MDPI 2019-12-18 /pmc/articles/PMC7022518/ /pubmed/31861293 http://dx.doi.org/10.3390/insects11010002 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Samerjai, Chutharat
Sukontason, Kabkaew L.
Sontigun, Narin
Sukontason, Kom
Klong-klaew, Tunwadee
Chareonviriyaphap, Theeraphap
Kurahashi, Hiromu
Klimpel, Sven
Kochmann, Judith
Saeung, Atiporn
Somboon, Pradya
Wannasan, Anchalee
Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand
title Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand
title_full Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand
title_fullStr Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand
title_full_unstemmed Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand
title_short Mitochondrial DNA-Based Identification of Forensically Important Flesh Flies (Diptera: Sarcophagidae) in Thailand
title_sort mitochondrial dna-based identification of forensically important flesh flies (diptera: sarcophagidae) in thailand
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022518/
https://www.ncbi.nlm.nih.gov/pubmed/31861293
http://dx.doi.org/10.3390/insects11010002
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