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Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases

Mycobacterial heat shock protein 65 gene (Hsp65) has been widely used for classification of Mycobacterial species, and detection of Mycobacterial genes by molecular methods and has proven useful in identification of Mycobacterial infection in various clinical conditions. Circulating antibody against...

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Autores principales: Zhang, Peilin, Minardi, Lawrence M., Kuenstner, John Todd, Zhang, Sylvia T., Zekan, Steve M., Kruzelock, Rusty
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022545/
https://www.ncbi.nlm.nih.gov/pubmed/31881708
http://dx.doi.org/10.3390/microorganisms8010047
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author Zhang, Peilin
Minardi, Lawrence M.
Kuenstner, John Todd
Zhang, Sylvia T.
Zekan, Steve M.
Kruzelock, Rusty
author_facet Zhang, Peilin
Minardi, Lawrence M.
Kuenstner, John Todd
Zhang, Sylvia T.
Zekan, Steve M.
Kruzelock, Rusty
author_sort Zhang, Peilin
collection PubMed
description Mycobacterial heat shock protein 65 gene (Hsp65) has been widely used for classification of Mycobacterial species, and detection of Mycobacterial genes by molecular methods and has proven useful in identification of Mycobacterial infection in various clinical conditions. Circulating antibody against Mycobacterial hsp65 has been found in many clinical diseases including autoimmune diseases (Crohn’s disease, lupus erythematosus, multiple sclerosis, diabetes, etc.), atherosclerosis and cancers. The prevalence of anti-Hsp65 antibody in the normal healthy population is unknown. We determined the blood levels of antibody against Mycobacterial hsp65 in the normal population represented by 288 blood donors of the American Red Cross and tested the blood of 109 patients with Crohn’s disease and 28 patients with Sjogren’s syndrome for comparison. The seroprevalence of anti-Hsp65 IgG in the normal population of Red Cross donors was 2.8% (8 of 288 positive). The Hsp65 antibody levels were significantly elevated in patients with Crohn’s disease and Sjogren’s syndrome. The prevalence of Hsp65 antibody in Crohn’s disease patients was 67.9% (74 of 109 patients), and 85.7% for Sjogren’s patients (24 of 28 patients). Our data indicate that anti-Hsp65 antibody is rare in the normal population, but frequent in chronic diseases. The presence of circulating Hsp65 antibody reflects an abnormal immune (adaptive) response to Mycobacterial exposure in patients with chronic diseases, thus differentiating the patients with chronic diseases from those clinical mimics.
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spelling pubmed-70225452020-03-09 Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases Zhang, Peilin Minardi, Lawrence M. Kuenstner, John Todd Zhang, Sylvia T. Zekan, Steve M. Kruzelock, Rusty Microorganisms Article Mycobacterial heat shock protein 65 gene (Hsp65) has been widely used for classification of Mycobacterial species, and detection of Mycobacterial genes by molecular methods and has proven useful in identification of Mycobacterial infection in various clinical conditions. Circulating antibody against Mycobacterial hsp65 has been found in many clinical diseases including autoimmune diseases (Crohn’s disease, lupus erythematosus, multiple sclerosis, diabetes, etc.), atherosclerosis and cancers. The prevalence of anti-Hsp65 antibody in the normal healthy population is unknown. We determined the blood levels of antibody against Mycobacterial hsp65 in the normal population represented by 288 blood donors of the American Red Cross and tested the blood of 109 patients with Crohn’s disease and 28 patients with Sjogren’s syndrome for comparison. The seroprevalence of anti-Hsp65 IgG in the normal population of Red Cross donors was 2.8% (8 of 288 positive). The Hsp65 antibody levels were significantly elevated in patients with Crohn’s disease and Sjogren’s syndrome. The prevalence of Hsp65 antibody in Crohn’s disease patients was 67.9% (74 of 109 patients), and 85.7% for Sjogren’s patients (24 of 28 patients). Our data indicate that anti-Hsp65 antibody is rare in the normal population, but frequent in chronic diseases. The presence of circulating Hsp65 antibody reflects an abnormal immune (adaptive) response to Mycobacterial exposure in patients with chronic diseases, thus differentiating the patients with chronic diseases from those clinical mimics. MDPI 2019-12-25 /pmc/articles/PMC7022545/ /pubmed/31881708 http://dx.doi.org/10.3390/microorganisms8010047 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Peilin
Minardi, Lawrence M.
Kuenstner, John Todd
Zhang, Sylvia T.
Zekan, Steve M.
Kruzelock, Rusty
Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases
title Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases
title_full Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases
title_fullStr Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases
title_full_unstemmed Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases
title_short Serological Testing for Mycobacterial Heat Shock Protein Hsp65 Antibody in Health and Diseases
title_sort serological testing for mycobacterial heat shock protein hsp65 antibody in health and diseases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022545/
https://www.ncbi.nlm.nih.gov/pubmed/31881708
http://dx.doi.org/10.3390/microorganisms8010047
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