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Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7

Bacillus licheniformis strain TAB7 is a bacterium used as a commercial deodorizing agent for compost in Japan. In this work, its ability to biotransform the following monocyclic phenolic compounds was assessed: ferulate, vanillate, p-coumarate, caffeate, protocatechuate, syringate, vanillin, and cin...

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Autores principales: Mpofu, Enock, Chakraborty, Joydeep, Suzuki-Minakuchi, Chiho, Okada, Kazunori, Kimura, Toshiaki, Nojiri, Hideaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022639/
https://www.ncbi.nlm.nih.gov/pubmed/31877822
http://dx.doi.org/10.3390/microorganisms8010026
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author Mpofu, Enock
Chakraborty, Joydeep
Suzuki-Minakuchi, Chiho
Okada, Kazunori
Kimura, Toshiaki
Nojiri, Hideaki
author_facet Mpofu, Enock
Chakraborty, Joydeep
Suzuki-Minakuchi, Chiho
Okada, Kazunori
Kimura, Toshiaki
Nojiri, Hideaki
author_sort Mpofu, Enock
collection PubMed
description Bacillus licheniformis strain TAB7 is a bacterium used as a commercial deodorizing agent for compost in Japan. In this work, its ability to biotransform the following monocyclic phenolic compounds was assessed: ferulate, vanillate, p-coumarate, caffeate, protocatechuate, syringate, vanillin, and cinnamate (a precursor for some phenolic compounds). These compounds are abundant in composting material and are reported to have allelopathic properties. They come from sources such as plant material decomposition or agro-industrial waste. Biotransformation assays were carried out in LB supplemented with 0.2 mg/mL of an individual phenolic compound and incubated for up to 15 days followed by extraction and HPLC analysis. The results showed that TAB7 could biotransform ferulate, caffeate, p-coumarate, vanillate, protocatechuate, and vanillin. It, however, had a poor ability to transform cinnamate and syringate. LC-MS/MS analysis showed that ferulate was transformed into 4-vinylguaiacol as the final product, while caffeate was transformed into 4-ethylcatechol. TAB7 genome analysis suggested that, while TAB7 may not mineralize phenolic compounds, it harbored genes possibly encoding phenolic acid decarboxylase, vanillate decarboxylase, and some protocatechuate degradation pathway enzymes, which are involved in the catabolism of phenolic compounds known to have negative allelopathy on some plants. The results thus suggested that TAB7 can reduce such phenolic compounds in compost.
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spelling pubmed-70226392020-03-09 Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7 Mpofu, Enock Chakraborty, Joydeep Suzuki-Minakuchi, Chiho Okada, Kazunori Kimura, Toshiaki Nojiri, Hideaki Microorganisms Article Bacillus licheniformis strain TAB7 is a bacterium used as a commercial deodorizing agent for compost in Japan. In this work, its ability to biotransform the following monocyclic phenolic compounds was assessed: ferulate, vanillate, p-coumarate, caffeate, protocatechuate, syringate, vanillin, and cinnamate (a precursor for some phenolic compounds). These compounds are abundant in composting material and are reported to have allelopathic properties. They come from sources such as plant material decomposition or agro-industrial waste. Biotransformation assays were carried out in LB supplemented with 0.2 mg/mL of an individual phenolic compound and incubated for up to 15 days followed by extraction and HPLC analysis. The results showed that TAB7 could biotransform ferulate, caffeate, p-coumarate, vanillate, protocatechuate, and vanillin. It, however, had a poor ability to transform cinnamate and syringate. LC-MS/MS analysis showed that ferulate was transformed into 4-vinylguaiacol as the final product, while caffeate was transformed into 4-ethylcatechol. TAB7 genome analysis suggested that, while TAB7 may not mineralize phenolic compounds, it harbored genes possibly encoding phenolic acid decarboxylase, vanillate decarboxylase, and some protocatechuate degradation pathway enzymes, which are involved in the catabolism of phenolic compounds known to have negative allelopathy on some plants. The results thus suggested that TAB7 can reduce such phenolic compounds in compost. MDPI 2019-12-21 /pmc/articles/PMC7022639/ /pubmed/31877822 http://dx.doi.org/10.3390/microorganisms8010026 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mpofu, Enock
Chakraborty, Joydeep
Suzuki-Minakuchi, Chiho
Okada, Kazunori
Kimura, Toshiaki
Nojiri, Hideaki
Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7
title Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7
title_full Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7
title_fullStr Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7
title_full_unstemmed Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7
title_short Biotransformation of Monocyclic Phenolic Compounds by Bacillus licheniformis TAB7
title_sort biotransformation of monocyclic phenolic compounds by bacillus licheniformis tab7
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022639/
https://www.ncbi.nlm.nih.gov/pubmed/31877822
http://dx.doi.org/10.3390/microorganisms8010026
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