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Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo

A hydrotrope-based pretreatment, benzenesulfonic acid (BA) pretreatment, was used to fractionate bamboo in this work. With optimized content (80 wt %) of BA in pretreatment liquor, about 90% of lignin and hemicellulose could be removed from bamboo under mild conditions (95 °C, 30 min or 80 °C, 60 mi...

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Autores principales: Luo, Xiaolin, Gong, Zhenggang, Shi, Jinghao, Chen, Lihui, Zhu, Wenyuan, Zhou, Yonghui, Huang, Liulian, Liu, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022729/
https://www.ncbi.nlm.nih.gov/pubmed/31936846
http://dx.doi.org/10.3390/polym12010191
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author Luo, Xiaolin
Gong, Zhenggang
Shi, Jinghao
Chen, Lihui
Zhu, Wenyuan
Zhou, Yonghui
Huang, Liulian
Liu, Jing
author_facet Luo, Xiaolin
Gong, Zhenggang
Shi, Jinghao
Chen, Lihui
Zhu, Wenyuan
Zhou, Yonghui
Huang, Liulian
Liu, Jing
author_sort Luo, Xiaolin
collection PubMed
description A hydrotrope-based pretreatment, benzenesulfonic acid (BA) pretreatment, was used to fractionate bamboo in this work. With optimized content (80 wt %) of BA in pretreatment liquor, about 90% of lignin and hemicellulose could be removed from bamboo under mild conditions (95 °C, 30 min or 80 °C, 60 min). The potential accessibility of BA pretreated substrate to cellulase was thus significantly improved and was also found to be much higher than those of acidic ethanol and dilute acid pretreatments. But the deposition of lignin on the surface of solid substrates, especially the BA pretreated substrate, was also observed, which showed a negative effect on the enzymatic hydrolysis efficiency. The addition of inexpensive soy protein, a bio-based lignin-shielding agent, could readily overcome this negative effect, leading the increase of enzymatic conversion of cellulose in BA pretreated substrate from 37% to 92% at a low cellulase loading of 4 FPU/g glucan. As compared to acidic ethanol and dilute acid pretreatments, the combination of BA pretreatment and soy protein could not only stably improve the efficiency of non-cellulose components removal, but also could significantly reduce the loading of cellulase.
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spelling pubmed-70227292020-03-11 Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo Luo, Xiaolin Gong, Zhenggang Shi, Jinghao Chen, Lihui Zhu, Wenyuan Zhou, Yonghui Huang, Liulian Liu, Jing Polymers (Basel) Article A hydrotrope-based pretreatment, benzenesulfonic acid (BA) pretreatment, was used to fractionate bamboo in this work. With optimized content (80 wt %) of BA in pretreatment liquor, about 90% of lignin and hemicellulose could be removed from bamboo under mild conditions (95 °C, 30 min or 80 °C, 60 min). The potential accessibility of BA pretreated substrate to cellulase was thus significantly improved and was also found to be much higher than those of acidic ethanol and dilute acid pretreatments. But the deposition of lignin on the surface of solid substrates, especially the BA pretreated substrate, was also observed, which showed a negative effect on the enzymatic hydrolysis efficiency. The addition of inexpensive soy protein, a bio-based lignin-shielding agent, could readily overcome this negative effect, leading the increase of enzymatic conversion of cellulose in BA pretreated substrate from 37% to 92% at a low cellulase loading of 4 FPU/g glucan. As compared to acidic ethanol and dilute acid pretreatments, the combination of BA pretreatment and soy protein could not only stably improve the efficiency of non-cellulose components removal, but also could significantly reduce the loading of cellulase. MDPI 2020-01-10 /pmc/articles/PMC7022729/ /pubmed/31936846 http://dx.doi.org/10.3390/polym12010191 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luo, Xiaolin
Gong, Zhenggang
Shi, Jinghao
Chen, Lihui
Zhu, Wenyuan
Zhou, Yonghui
Huang, Liulian
Liu, Jing
Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo
title Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo
title_full Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo
title_fullStr Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo
title_full_unstemmed Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo
title_short Integrating Benzenesulfonic Acid Pretreatment and Bio-Based Lignin-Shielding Agent for Robust Enzymatic Conversion of Cellulose in Bamboo
title_sort integrating benzenesulfonic acid pretreatment and bio-based lignin-shielding agent for robust enzymatic conversion of cellulose in bamboo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022729/
https://www.ncbi.nlm.nih.gov/pubmed/31936846
http://dx.doi.org/10.3390/polym12010191
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