Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)

The lipidome of royal jelly (RJ) consists of medium-chained (8–12 carbon atoms) free fatty acids. We present herein a liquid chromatography-high resolution mass spectrometry (HRMS) method that permits the determination of RJ fatty acids and at the same time the detection of suspect fatty acids. The...

Descripción completa

Detalles Bibliográficos
Autores principales: Kokotou, Maroula G., Mantzourani, Christiana, Babaiti, Rodalia, Kokotos, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022826/
https://www.ncbi.nlm.nih.gov/pubmed/31963373
http://dx.doi.org/10.3390/metabo10010040
_version_ 1783498106678018048
author Kokotou, Maroula G.
Mantzourani, Christiana
Babaiti, Rodalia
Kokotos, George
author_facet Kokotou, Maroula G.
Mantzourani, Christiana
Babaiti, Rodalia
Kokotos, George
author_sort Kokotou, Maroula G.
collection PubMed
description The lipidome of royal jelly (RJ) consists of medium-chained (8–12 carbon atoms) free fatty acids. We present herein a liquid chromatography-high resolution mass spectrometry (HRMS) method that permits the determination of RJ fatty acids and at the same time the detection of suspect fatty acids. The method allows for the direct quantification of seven free fatty acids of RJ, avoiding any derivatization step. It was validated and applied in seven RJ samples, where the major RJ fatty acid trans-10-hydroxy-2-decenoic acid (10-HDA) was found to vary from 0.771 ± 0.08 to 0.928 ± 0.04 g/100 g fresh RJ. Four additional suspect fatty acids were simultaneously detected taking advantage of the HRMS detection.
format Online
Article
Text
id pubmed-7022826
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70228262020-03-11 Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) Kokotou, Maroula G. Mantzourani, Christiana Babaiti, Rodalia Kokotos, George Metabolites Article The lipidome of royal jelly (RJ) consists of medium-chained (8–12 carbon atoms) free fatty acids. We present herein a liquid chromatography-high resolution mass spectrometry (HRMS) method that permits the determination of RJ fatty acids and at the same time the detection of suspect fatty acids. The method allows for the direct quantification of seven free fatty acids of RJ, avoiding any derivatization step. It was validated and applied in seven RJ samples, where the major RJ fatty acid trans-10-hydroxy-2-decenoic acid (10-HDA) was found to vary from 0.771 ± 0.08 to 0.928 ± 0.04 g/100 g fresh RJ. Four additional suspect fatty acids were simultaneously detected taking advantage of the HRMS detection. MDPI 2020-01-16 /pmc/articles/PMC7022826/ /pubmed/31963373 http://dx.doi.org/10.3390/metabo10010040 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kokotou, Maroula G.
Mantzourani, Christiana
Babaiti, Rodalia
Kokotos, George
Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
title Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
title_full Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
title_fullStr Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
title_full_unstemmed Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
title_short Study of the Royal Jelly Free Fatty Acids by Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS)
title_sort study of the royal jelly free fatty acids by liquid chromatography-high resolution mass spectrometry (lc-hrms)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022826/
https://www.ncbi.nlm.nih.gov/pubmed/31963373
http://dx.doi.org/10.3390/metabo10010040
work_keys_str_mv AT kokotoumaroulag studyoftheroyaljellyfreefattyacidsbyliquidchromatographyhighresolutionmassspectrometrylchrms
AT mantzouranichristiana studyoftheroyaljellyfreefattyacidsbyliquidchromatographyhighresolutionmassspectrometrylchrms
AT babaitirodalia studyoftheroyaljellyfreefattyacidsbyliquidchromatographyhighresolutionmassspectrometrylchrms
AT kokotosgeorge studyoftheroyaljellyfreefattyacidsbyliquidchromatographyhighresolutionmassspectrometrylchrms

Ejemplares similares