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Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro

Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived...

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Autores principales: Rocha-Ramírez, Luz María, Hernández-Ochoa, Beatriz, Gómez-Manzo, Saúl, Marcial-Quino, Jaime, Cárdenas-Rodríguez, Noemí, Centeno-Leija, Sara, García-Garibay, Mariano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022880/
https://www.ncbi.nlm.nih.gov/pubmed/31936101
http://dx.doi.org/10.3390/microorganisms8010079
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author Rocha-Ramírez, Luz María
Hernández-Ochoa, Beatriz
Gómez-Manzo, Saúl
Marcial-Quino, Jaime
Cárdenas-Rodríguez, Noemí
Centeno-Leija, Sara
García-Garibay, Mariano
author_facet Rocha-Ramírez, Luz María
Hernández-Ochoa, Beatriz
Gómez-Manzo, Saúl
Marcial-Quino, Jaime
Cárdenas-Rodríguez, Noemí
Centeno-Leija, Sara
García-Garibay, Mariano
author_sort Rocha-Ramírez, Luz María
collection PubMed
description Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-β) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain.
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spelling pubmed-70228802020-03-12 Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro Rocha-Ramírez, Luz María Hernández-Ochoa, Beatriz Gómez-Manzo, Saúl Marcial-Quino, Jaime Cárdenas-Rodríguez, Noemí Centeno-Leija, Sara García-Garibay, Mariano Microorganisms Article Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-β) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain. MDPI 2020-01-07 /pmc/articles/PMC7022880/ /pubmed/31936101 http://dx.doi.org/10.3390/microorganisms8010079 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rocha-Ramírez, Luz María
Hernández-Ochoa, Beatriz
Gómez-Manzo, Saúl
Marcial-Quino, Jaime
Cárdenas-Rodríguez, Noemí
Centeno-Leija, Sara
García-Garibay, Mariano
Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
title Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
title_full Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
title_fullStr Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
title_full_unstemmed Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
title_short Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
title_sort evaluation of immunomodulatory activities of the heat-killed probiotic strain lactobacillus casei imau60214 on macrophages in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022880/
https://www.ncbi.nlm.nih.gov/pubmed/31936101
http://dx.doi.org/10.3390/microorganisms8010079
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