Cargando…
Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro
Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022880/ https://www.ncbi.nlm.nih.gov/pubmed/31936101 http://dx.doi.org/10.3390/microorganisms8010079 |
_version_ | 1783498119839744000 |
---|---|
author | Rocha-Ramírez, Luz María Hernández-Ochoa, Beatriz Gómez-Manzo, Saúl Marcial-Quino, Jaime Cárdenas-Rodríguez, Noemí Centeno-Leija, Sara García-Garibay, Mariano |
author_facet | Rocha-Ramírez, Luz María Hernández-Ochoa, Beatriz Gómez-Manzo, Saúl Marcial-Quino, Jaime Cárdenas-Rodríguez, Noemí Centeno-Leija, Sara García-Garibay, Mariano |
author_sort | Rocha-Ramírez, Luz María |
collection | PubMed |
description | Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-β) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain. |
format | Online Article Text |
id | pubmed-7022880 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70228802020-03-12 Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro Rocha-Ramírez, Luz María Hernández-Ochoa, Beatriz Gómez-Manzo, Saúl Marcial-Quino, Jaime Cárdenas-Rodríguez, Noemí Centeno-Leija, Sara García-Garibay, Mariano Microorganisms Article Most Lactobacillus species have beneficial immunological (“immunoprobiotic”) effects in the host. However, it is unclear how probiotic bacteria regulate immune responses. The present study investigated the effects of heat-killed Lactobacillus casei IMAU60214 on the activity of human monocyte-derived macrophages (MDMs). Human MDMs were treated with heat-killed L. casei at a ratio (bacteria/MDM) of 50:1, 100:1, 250:1, and 500:1, and then evaluated for the following: NO production, by Griess reaction; phagocytosis of FITC-labeled Staphylococcus aureus particles; cytokine secretion profile (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-12p70, IL-10, and transforming growth factor (TGF)-β) by ELISA; and costimulatory molecule (CD80 and CD86) surface expression, by flow cytometry. Heat-killed L. casei IMAU60214 enhanced phagocytosis, NO production, cytokine release, and surface expression of CD80 and CD86 in a dose-dependent manner. All products were previously suppressed by pretreatment with a Toll-like receptor 2 (TLR2)-neutralizing antibody. Overall, our findings suggest that this probiotic strain promotes an M1-like pro-inflammatory phenotype through the TLR2 signaling pathway. These effects on macrophage phenotype help explain the probiotic efficacy of Lactobacillus and provide important information for the selection of therapeutic targets and treatments compatible with the immunological characteristics of this probiotic strain. MDPI 2020-01-07 /pmc/articles/PMC7022880/ /pubmed/31936101 http://dx.doi.org/10.3390/microorganisms8010079 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rocha-Ramírez, Luz María Hernández-Ochoa, Beatriz Gómez-Manzo, Saúl Marcial-Quino, Jaime Cárdenas-Rodríguez, Noemí Centeno-Leija, Sara García-Garibay, Mariano Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro |
title | Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro |
title_full | Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro |
title_fullStr | Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro |
title_full_unstemmed | Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro |
title_short | Evaluation of Immunomodulatory Activities of the Heat-Killed Probiotic Strain Lactobacillus casei IMAU60214 on Macrophages In Vitro |
title_sort | evaluation of immunomodulatory activities of the heat-killed probiotic strain lactobacillus casei imau60214 on macrophages in vitro |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7022880/ https://www.ncbi.nlm.nih.gov/pubmed/31936101 http://dx.doi.org/10.3390/microorganisms8010079 |
work_keys_str_mv | AT rocharamirezluzmaria evaluationofimmunomodulatoryactivitiesoftheheatkilledprobioticstrainlactobacilluscaseiimau60214onmacrophagesinvitro AT hernandezochoabeatriz evaluationofimmunomodulatoryactivitiesoftheheatkilledprobioticstrainlactobacilluscaseiimau60214onmacrophagesinvitro AT gomezmanzosaul evaluationofimmunomodulatoryactivitiesoftheheatkilledprobioticstrainlactobacilluscaseiimau60214onmacrophagesinvitro AT marcialquinojaime evaluationofimmunomodulatoryactivitiesoftheheatkilledprobioticstrainlactobacilluscaseiimau60214onmacrophagesinvitro AT cardenasrodrigueznoemi evaluationofimmunomodulatoryactivitiesoftheheatkilledprobioticstrainlactobacilluscaseiimau60214onmacrophagesinvitro AT centenoleijasara evaluationofimmunomodulatoryactivitiesoftheheatkilledprobioticstrainlactobacilluscaseiimau60214onmacrophagesinvitro AT garciagaribaymariano evaluationofimmunomodulatoryactivitiesoftheheatkilledprobioticstrainlactobacilluscaseiimau60214onmacrophagesinvitro |