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Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

Plant secondary metabolites including phenolics and flavonoidsare synthesized through phenylpropanoid and phenylpropanoid–acetate pathways and significantly contribute against adverse effect of abiotic and biotic stresses. Herein, we present the development and execution of a novel and expeditious u...

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Autores principales: Mandhania, Shiwani, Pal, Ajay, Saharan, Vinod
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023218/
https://www.ncbi.nlm.nih.gov/pubmed/31861330
http://dx.doi.org/10.3390/biom10010006
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author Mandhania, Shiwani
Pal, Ajay
Saharan, Vinod
author_facet Mandhania, Shiwani
Pal, Ajay
Saharan, Vinod
author_sort Mandhania, Shiwani
collection PubMed
description Plant secondary metabolites including phenolics and flavonoidsare synthesized through phenylpropanoid and phenylpropanoid–acetate pathways and significantly contribute against adverse effect of abiotic and biotic stresses. Herein, we present the development and execution of a novel and expeditious ultra-performance liquid chromatographic-photodiode array (UPLC–PDA) method for qualitative and quantitative analysis of 28 phenolic compounds comprising of flavonoids, phenolic acids, aldehydes and alcohols. The method is able to separate phenolic compounds in just 17 min with the separation of isobaric species such as 3,4 dihydroxybenzoic acid and 3,5 dihydroxy benzoic acid; quercetin and taxifolin. Linear curves concentrations ranged from 6–18 µg/mL (3,5 dihydroxy benzoic acid), 4–12 µg/mL (catechin and salicylic acid) and 2–6 µg/mL for rest of the compounds and correlation coefficients were >0.994. The limit of detection (LOD) varied from 0.04–0.45 µg/mL. Cotton root samples were used to assess the method in terms of recovery efficiency (85–120%), precision (0.12–4.09%) and intermediate precision (0.32–4.0%).Phenolics and flavonoidsin root samples of healthy and diseased plants as well as leaf samples of healthy plants were successfully quantified using this novel method without an expensive Mass Spectrometer.
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spelling pubmed-70232182020-03-12 Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector Mandhania, Shiwani Pal, Ajay Saharan, Vinod Biomolecules Article Plant secondary metabolites including phenolics and flavonoidsare synthesized through phenylpropanoid and phenylpropanoid–acetate pathways and significantly contribute against adverse effect of abiotic and biotic stresses. Herein, we present the development and execution of a novel and expeditious ultra-performance liquid chromatographic-photodiode array (UPLC–PDA) method for qualitative and quantitative analysis of 28 phenolic compounds comprising of flavonoids, phenolic acids, aldehydes and alcohols. The method is able to separate phenolic compounds in just 17 min with the separation of isobaric species such as 3,4 dihydroxybenzoic acid and 3,5 dihydroxy benzoic acid; quercetin and taxifolin. Linear curves concentrations ranged from 6–18 µg/mL (3,5 dihydroxy benzoic acid), 4–12 µg/mL (catechin and salicylic acid) and 2–6 µg/mL for rest of the compounds and correlation coefficients were >0.994. The limit of detection (LOD) varied from 0.04–0.45 µg/mL. Cotton root samples were used to assess the method in terms of recovery efficiency (85–120%), precision (0.12–4.09%) and intermediate precision (0.32–4.0%).Phenolics and flavonoidsin root samples of healthy and diseased plants as well as leaf samples of healthy plants were successfully quantified using this novel method without an expensive Mass Spectrometer. MDPI 2019-12-18 /pmc/articles/PMC7023218/ /pubmed/31861330 http://dx.doi.org/10.3390/biom10010006 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mandhania, Shiwani
Pal, Ajay
Saharan, Vinod
Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector
title Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector
title_full Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector
title_fullStr Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector
title_full_unstemmed Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector
title_short Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector
title_sort simultaneous estimation of twenty eight phenolic compounds by a novel and expeditious method developed on quaternary ultra-performance liquid chromatography system with a photodiode array detector
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023218/
https://www.ncbi.nlm.nih.gov/pubmed/31861330
http://dx.doi.org/10.3390/biom10010006
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