Modified Protocol of Harvesting, Extraction, and Normalization Approaches for Gas Chromatography Mass Spectrometry-Based Metabolomics Analysis of Adherent Cells Grown Under High Fetal Calf Serum Conditions

A gas chromatography mass spectrometry (GC-MS) metabolomics protocol was modified for quenching, harvesting, and extraction of metabolites from adherent cells grown under high (20%) fetal calf serum conditions. The reproducibility of using either 50% or 80% methanol for quenching of cells was compar...

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Detalles Bibliográficos
Autores principales: Fritsche-Guenther, Raphaela, Bauer, Anna, Gloaguen, Yoann, Lorenz, Mario, Kirwan, Jennifer A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023238/
https://www.ncbi.nlm.nih.gov/pubmed/31861324
http://dx.doi.org/10.3390/metabo10010002

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023238/
https://www.ncbi.nlm.nih.gov/pubmed/31861324
http://dx.doi.org/10.3390/metabo10010002

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