Cargando…

A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies

Background and Objectives: Autologous and allogeneic stem cell transplantation is used in the treatment of high-risk hematological malignancies, and monocytes are probably involved in hematological reconstitution as well as posttransplant immunoregulation. The aim of our study was to investigate the...

Descripción completa

Detalles Bibliográficos
Autores principales: Rundgren, Ida Marie, Ersvær, Elisabeth, Ahmed, Aymen Bushra, Ryningen, Anita, Bruserud, Øystein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023283/
https://www.ncbi.nlm.nih.gov/pubmed/31963675
http://dx.doi.org/10.3390/medicina56010036
_version_ 1783498214221021184
author Rundgren, Ida Marie
Ersvær, Elisabeth
Ahmed, Aymen Bushra
Ryningen, Anita
Bruserud, Øystein
author_facet Rundgren, Ida Marie
Ersvær, Elisabeth
Ahmed, Aymen Bushra
Ryningen, Anita
Bruserud, Øystein
author_sort Rundgren, Ida Marie
collection PubMed
description Background and Objectives: Autologous and allogeneic stem cell transplantation is used in the treatment of high-risk hematological malignancies, and monocytes are probably involved in hematological reconstitution as well as posttransplant immunoregulation. The aim of our study was to investigate the levels of circulating monocyte subsets in allotransplant recipients. Materials and Methods: The levels of the classical, intermediate, and nonclassical monocyte subsets were determined by flow cytometry. Sixteen patients and 18 healthy controls were included, and the levels were analyzed during pretransplant remission (n = 13), early posttransplant during cytopenia (n = 9), and early reconstitution (n = 9). Results: Most patients in remission showed a majority of classical monocytes. The patients showed severe early posttransplant monocytopenia, but the total peripheral blood monocyte counts normalized very early on, and before neutrophil and platelet counts. During the first 7–10 days posttransplant (i.e., during cytopenia) a majority of the circulating monocytes showed a nonclassical phenotype, but later (i.e., 12–28 days posttransplant) the majority showed a classical phenotype. However, the variation range of classical monocytes was wider for patients in remission and during regeneration than for healthy controls. Conclusions: The total peripheral blood monocyte levels normalize at the very early stages and before neutrophil reconstitution after stem cell transplantation, and a dominance of classical monocytes is reached within 2–4 weeks posttransplant.
format Online
Article
Text
id pubmed-7023283
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70232832020-03-12 A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies Rundgren, Ida Marie Ersvær, Elisabeth Ahmed, Aymen Bushra Ryningen, Anita Bruserud, Øystein Medicina (Kaunas) Article Background and Objectives: Autologous and allogeneic stem cell transplantation is used in the treatment of high-risk hematological malignancies, and monocytes are probably involved in hematological reconstitution as well as posttransplant immunoregulation. The aim of our study was to investigate the levels of circulating monocyte subsets in allotransplant recipients. Materials and Methods: The levels of the classical, intermediate, and nonclassical monocyte subsets were determined by flow cytometry. Sixteen patients and 18 healthy controls were included, and the levels were analyzed during pretransplant remission (n = 13), early posttransplant during cytopenia (n = 9), and early reconstitution (n = 9). Results: Most patients in remission showed a majority of classical monocytes. The patients showed severe early posttransplant monocytopenia, but the total peripheral blood monocyte counts normalized very early on, and before neutrophil and platelet counts. During the first 7–10 days posttransplant (i.e., during cytopenia) a majority of the circulating monocytes showed a nonclassical phenotype, but later (i.e., 12–28 days posttransplant) the majority showed a classical phenotype. However, the variation range of classical monocytes was wider for patients in remission and during regeneration than for healthy controls. Conclusions: The total peripheral blood monocyte levels normalize at the very early stages and before neutrophil reconstitution after stem cell transplantation, and a dominance of classical monocytes is reached within 2–4 weeks posttransplant. MDPI 2020-01-18 /pmc/articles/PMC7023283/ /pubmed/31963675 http://dx.doi.org/10.3390/medicina56010036 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rundgren, Ida Marie
Ersvær, Elisabeth
Ahmed, Aymen Bushra
Ryningen, Anita
Bruserud, Øystein
A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies
title A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies
title_full A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies
title_fullStr A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies
title_full_unstemmed A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies
title_short A Pilot Study of Circulating Monocyte Subsets in Patients Treated with Stem Cell Transplantation for High-Risk Hematological Malignancies
title_sort pilot study of circulating monocyte subsets in patients treated with stem cell transplantation for high-risk hematological malignancies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023283/
https://www.ncbi.nlm.nih.gov/pubmed/31963675
http://dx.doi.org/10.3390/medicina56010036
work_keys_str_mv AT rundgrenidamarie apilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT ersværelisabeth apilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT ahmedaymenbushra apilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT ryningenanita apilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT bruserudøystein apilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT rundgrenidamarie pilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT ersværelisabeth pilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT ahmedaymenbushra pilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT ryningenanita pilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies
AT bruserudøystein pilotstudyofcirculatingmonocytesubsetsinpatientstreatedwithstemcelltransplantationforhighriskhematologicalmalignancies