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Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
Methicillin-resistant Staphylococcus aureus (MRSA) is a common human pathogen that causes several difficult-to-treat infections, including biofilm-associated infections. The biofilm-forming ability of S. aureus plays a pivotal role in its resistance to most currently available antibiotics, including...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023310/ https://www.ncbi.nlm.nih.gov/pubmed/31936809 http://dx.doi.org/10.3390/microorganisms8010099 |
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author | Yuan, Zhongwei Dai, Yuyun Ouyang, Ping Rehman, Tayyab Hussain, Sajjad Zhang, Tianyi Yin, Zhongqiong Fu, Hualin Lin, Juchun He, Changliang Lv, Cheng Liang, Xiaoxia Shu, Gang Song, Xu Li, Lixia Zou, Yuanfeng Yin, Lizi |
author_facet | Yuan, Zhongwei Dai, Yuyun Ouyang, Ping Rehman, Tayyab Hussain, Sajjad Zhang, Tianyi Yin, Zhongqiong Fu, Hualin Lin, Juchun He, Changliang Lv, Cheng Liang, Xiaoxia Shu, Gang Song, Xu Li, Lixia Zou, Yuanfeng Yin, Lizi |
author_sort | Yuan, Zhongwei |
collection | PubMed |
description | Methicillin-resistant Staphylococcus aureus (MRSA) is a common human pathogen that causes several difficult-to-treat infections, including biofilm-associated infections. The biofilm-forming ability of S. aureus plays a pivotal role in its resistance to most currently available antibiotics, including vancomycin, which is the first-choice drug for treating MRSA infections. In this study, the ability of thymol (a monoterpenoid phenol isolated from plants) to inhibit biofilm formation and to eliminate mature biofilms, was assessed. We found that thymol could inhibit biofilm formation and remove mature biofilms by inhibiting the production of polysaccharide intracellular adhesin (PIA) and the release of extracellular DNA (eDNA). However, cotreatment with thymol and vancomycin was more effective at eliminating MRSA biofilms, in a mouse infection model, than monotherapy with vancomycin. Comparative histopathological analyses revealed that thymol reduced the pathological changes and inflammatory responses in the wounds. Assessments of white blood cell counts and serum TNF-α and IL-6 levels showed reduced inflammation and an increased immune response following treatment with thymol and vancomycin. These results indicate that combinatorial treatment with thymol and vancomycin has the potential to serve as a more effective therapy for MRSA biofilm-associated infections than vancomycin monotherapy. |
format | Online Article Text |
id | pubmed-7023310 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70233102020-03-12 Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model Yuan, Zhongwei Dai, Yuyun Ouyang, Ping Rehman, Tayyab Hussain, Sajjad Zhang, Tianyi Yin, Zhongqiong Fu, Hualin Lin, Juchun He, Changliang Lv, Cheng Liang, Xiaoxia Shu, Gang Song, Xu Li, Lixia Zou, Yuanfeng Yin, Lizi Microorganisms Article Methicillin-resistant Staphylococcus aureus (MRSA) is a common human pathogen that causes several difficult-to-treat infections, including biofilm-associated infections. The biofilm-forming ability of S. aureus plays a pivotal role in its resistance to most currently available antibiotics, including vancomycin, which is the first-choice drug for treating MRSA infections. In this study, the ability of thymol (a monoterpenoid phenol isolated from plants) to inhibit biofilm formation and to eliminate mature biofilms, was assessed. We found that thymol could inhibit biofilm formation and remove mature biofilms by inhibiting the production of polysaccharide intracellular adhesin (PIA) and the release of extracellular DNA (eDNA). However, cotreatment with thymol and vancomycin was more effective at eliminating MRSA biofilms, in a mouse infection model, than monotherapy with vancomycin. Comparative histopathological analyses revealed that thymol reduced the pathological changes and inflammatory responses in the wounds. Assessments of white blood cell counts and serum TNF-α and IL-6 levels showed reduced inflammation and an increased immune response following treatment with thymol and vancomycin. These results indicate that combinatorial treatment with thymol and vancomycin has the potential to serve as a more effective therapy for MRSA biofilm-associated infections than vancomycin monotherapy. MDPI 2020-01-10 /pmc/articles/PMC7023310/ /pubmed/31936809 http://dx.doi.org/10.3390/microorganisms8010099 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yuan, Zhongwei Dai, Yuyun Ouyang, Ping Rehman, Tayyab Hussain, Sajjad Zhang, Tianyi Yin, Zhongqiong Fu, Hualin Lin, Juchun He, Changliang Lv, Cheng Liang, Xiaoxia Shu, Gang Song, Xu Li, Lixia Zou, Yuanfeng Yin, Lizi Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model |
title | Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model |
title_full | Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model |
title_fullStr | Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model |
title_full_unstemmed | Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model |
title_short | Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model |
title_sort | thymol inhibits biofilm formation, eliminates pre-existing biofilms, and enhances clearance of methicillin-resistant staphylococcus aureus (mrsa) in a mouse peritoneal implant infection model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023310/ https://www.ncbi.nlm.nih.gov/pubmed/31936809 http://dx.doi.org/10.3390/microorganisms8010099 |
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