Cargando…

Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model

Methicillin-resistant Staphylococcus aureus (MRSA) is a common human pathogen that causes several difficult-to-treat infections, including biofilm-associated infections. The biofilm-forming ability of S. aureus plays a pivotal role in its resistance to most currently available antibiotics, including...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Zhongwei, Dai, Yuyun, Ouyang, Ping, Rehman, Tayyab, Hussain, Sajjad, Zhang, Tianyi, Yin, Zhongqiong, Fu, Hualin, Lin, Juchun, He, Changliang, Lv, Cheng, Liang, Xiaoxia, Shu, Gang, Song, Xu, Li, Lixia, Zou, Yuanfeng, Yin, Lizi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023310/
https://www.ncbi.nlm.nih.gov/pubmed/31936809
http://dx.doi.org/10.3390/microorganisms8010099
_version_ 1783498219792105472
author Yuan, Zhongwei
Dai, Yuyun
Ouyang, Ping
Rehman, Tayyab
Hussain, Sajjad
Zhang, Tianyi
Yin, Zhongqiong
Fu, Hualin
Lin, Juchun
He, Changliang
Lv, Cheng
Liang, Xiaoxia
Shu, Gang
Song, Xu
Li, Lixia
Zou, Yuanfeng
Yin, Lizi
author_facet Yuan, Zhongwei
Dai, Yuyun
Ouyang, Ping
Rehman, Tayyab
Hussain, Sajjad
Zhang, Tianyi
Yin, Zhongqiong
Fu, Hualin
Lin, Juchun
He, Changliang
Lv, Cheng
Liang, Xiaoxia
Shu, Gang
Song, Xu
Li, Lixia
Zou, Yuanfeng
Yin, Lizi
author_sort Yuan, Zhongwei
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) is a common human pathogen that causes several difficult-to-treat infections, including biofilm-associated infections. The biofilm-forming ability of S. aureus plays a pivotal role in its resistance to most currently available antibiotics, including vancomycin, which is the first-choice drug for treating MRSA infections. In this study, the ability of thymol (a monoterpenoid phenol isolated from plants) to inhibit biofilm formation and to eliminate mature biofilms, was assessed. We found that thymol could inhibit biofilm formation and remove mature biofilms by inhibiting the production of polysaccharide intracellular adhesin (PIA) and the release of extracellular DNA (eDNA). However, cotreatment with thymol and vancomycin was more effective at eliminating MRSA biofilms, in a mouse infection model, than monotherapy with vancomycin. Comparative histopathological analyses revealed that thymol reduced the pathological changes and inflammatory responses in the wounds. Assessments of white blood cell counts and serum TNF-α and IL-6 levels showed reduced inflammation and an increased immune response following treatment with thymol and vancomycin. These results indicate that combinatorial treatment with thymol and vancomycin has the potential to serve as a more effective therapy for MRSA biofilm-associated infections than vancomycin monotherapy.
format Online
Article
Text
id pubmed-7023310
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70233102020-03-12 Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model Yuan, Zhongwei Dai, Yuyun Ouyang, Ping Rehman, Tayyab Hussain, Sajjad Zhang, Tianyi Yin, Zhongqiong Fu, Hualin Lin, Juchun He, Changliang Lv, Cheng Liang, Xiaoxia Shu, Gang Song, Xu Li, Lixia Zou, Yuanfeng Yin, Lizi Microorganisms Article Methicillin-resistant Staphylococcus aureus (MRSA) is a common human pathogen that causes several difficult-to-treat infections, including biofilm-associated infections. The biofilm-forming ability of S. aureus plays a pivotal role in its resistance to most currently available antibiotics, including vancomycin, which is the first-choice drug for treating MRSA infections. In this study, the ability of thymol (a monoterpenoid phenol isolated from plants) to inhibit biofilm formation and to eliminate mature biofilms, was assessed. We found that thymol could inhibit biofilm formation and remove mature biofilms by inhibiting the production of polysaccharide intracellular adhesin (PIA) and the release of extracellular DNA (eDNA). However, cotreatment with thymol and vancomycin was more effective at eliminating MRSA biofilms, in a mouse infection model, than monotherapy with vancomycin. Comparative histopathological analyses revealed that thymol reduced the pathological changes and inflammatory responses in the wounds. Assessments of white blood cell counts and serum TNF-α and IL-6 levels showed reduced inflammation and an increased immune response following treatment with thymol and vancomycin. These results indicate that combinatorial treatment with thymol and vancomycin has the potential to serve as a more effective therapy for MRSA biofilm-associated infections than vancomycin monotherapy. MDPI 2020-01-10 /pmc/articles/PMC7023310/ /pubmed/31936809 http://dx.doi.org/10.3390/microorganisms8010099 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuan, Zhongwei
Dai, Yuyun
Ouyang, Ping
Rehman, Tayyab
Hussain, Sajjad
Zhang, Tianyi
Yin, Zhongqiong
Fu, Hualin
Lin, Juchun
He, Changliang
Lv, Cheng
Liang, Xiaoxia
Shu, Gang
Song, Xu
Li, Lixia
Zou, Yuanfeng
Yin, Lizi
Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
title Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
title_full Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
title_fullStr Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
title_full_unstemmed Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
title_short Thymol Inhibits Biofilm Formation, Eliminates Pre-Existing Biofilms, and Enhances Clearance of Methicillin-Resistant Staphylococcus aureus (MRSA) in a Mouse Peritoneal Implant Infection Model
title_sort thymol inhibits biofilm formation, eliminates pre-existing biofilms, and enhances clearance of methicillin-resistant staphylococcus aureus (mrsa) in a mouse peritoneal implant infection model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023310/
https://www.ncbi.nlm.nih.gov/pubmed/31936809
http://dx.doi.org/10.3390/microorganisms8010099
work_keys_str_mv AT yuanzhongwei thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT daiyuyun thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT ouyangping thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT rehmantayyab thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT hussainsajjad thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT zhangtianyi thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT yinzhongqiong thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT fuhualin thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT linjuchun thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT hechangliang thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT lvcheng thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT liangxiaoxia thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT shugang thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT songxu thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT lilixia thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT zouyuanfeng thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel
AT yinlizi thymolinhibitsbiofilmformationeliminatespreexistingbiofilmsandenhancesclearanceofmethicillinresistantstaphylococcusaureusmrsainamouseperitonealimplantinfectionmodel