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Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C

Myticin C is the most studied antimicrobial peptide in the marine mussel Mytilus galloprovincialis. Although it is constitutively expressed in mussel hemocytes and displays antibacterial, antiviral, and chemotactic functions, recent work has suggested that this molecule is mainly activated after tis...

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Autores principales: Rey-Campos, Magalí, Moreira, Rebeca, Romero, Alejandro, Medina-Gali, Regla M., Novoa, Beatriz, Gasset, María, Figueras, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023338/
https://www.ncbi.nlm.nih.gov/pubmed/31947557
http://dx.doi.org/10.3390/biom10010133
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author Rey-Campos, Magalí
Moreira, Rebeca
Romero, Alejandro
Medina-Gali, Regla M.
Novoa, Beatriz
Gasset, María
Figueras, Antonio
author_facet Rey-Campos, Magalí
Moreira, Rebeca
Romero, Alejandro
Medina-Gali, Regla M.
Novoa, Beatriz
Gasset, María
Figueras, Antonio
author_sort Rey-Campos, Magalí
collection PubMed
description Myticin C is the most studied antimicrobial peptide in the marine mussel Mytilus galloprovincialis. Although it is constitutively expressed in mussel hemocytes and displays antibacterial, antiviral, and chemotactic functions, recent work has suggested that this molecule is mainly activated after tissue injury. Therefore, the main objective of this work was to characterize the hemocytes’ transcriptomic response after a myticin C treatment, in order to understand the molecular changes induced by this cytokine-like molecule. The transcriptome analysis revealed the modulation of genes related to cellular movement, such as myosin, transgelin, and calponin-like proteins, in agreement with results of functional assays, where an implication of myticin C in the in vitro activation of hemocytes and migration was evidenced. This was also observed in vivo after a tissue injury, when hemocytes, with high concentrations of myticin C, migrated to the damaged area to heal the wound. All these properties allowed us to think about the biotechnological application of these molecules as wound healers. Human keratinocytes and larvae zebrafish models were used to confirm this hypothesis. Accelerated regeneration after a wound or tail fin amputation was observed after treatment with the myticin C peptide, supporting the chemotactic and healing activity of myticin C.
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spelling pubmed-70233382020-03-12 Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C Rey-Campos, Magalí Moreira, Rebeca Romero, Alejandro Medina-Gali, Regla M. Novoa, Beatriz Gasset, María Figueras, Antonio Biomolecules Article Myticin C is the most studied antimicrobial peptide in the marine mussel Mytilus galloprovincialis. Although it is constitutively expressed in mussel hemocytes and displays antibacterial, antiviral, and chemotactic functions, recent work has suggested that this molecule is mainly activated after tissue injury. Therefore, the main objective of this work was to characterize the hemocytes’ transcriptomic response after a myticin C treatment, in order to understand the molecular changes induced by this cytokine-like molecule. The transcriptome analysis revealed the modulation of genes related to cellular movement, such as myosin, transgelin, and calponin-like proteins, in agreement with results of functional assays, where an implication of myticin C in the in vitro activation of hemocytes and migration was evidenced. This was also observed in vivo after a tissue injury, when hemocytes, with high concentrations of myticin C, migrated to the damaged area to heal the wound. All these properties allowed us to think about the biotechnological application of these molecules as wound healers. Human keratinocytes and larvae zebrafish models were used to confirm this hypothesis. Accelerated regeneration after a wound or tail fin amputation was observed after treatment with the myticin C peptide, supporting the chemotactic and healing activity of myticin C. MDPI 2020-01-14 /pmc/articles/PMC7023338/ /pubmed/31947557 http://dx.doi.org/10.3390/biom10010133 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rey-Campos, Magalí
Moreira, Rebeca
Romero, Alejandro
Medina-Gali, Regla M.
Novoa, Beatriz
Gasset, María
Figueras, Antonio
Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C
title Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C
title_full Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C
title_fullStr Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C
title_full_unstemmed Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C
title_short Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C
title_sort transcriptomic analysis reveals the wound healing activity of mussel myticin c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023338/
https://www.ncbi.nlm.nih.gov/pubmed/31947557
http://dx.doi.org/10.3390/biom10010133
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