Cargando…
Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature
Cell Separation is important in various biomedical fields. We have prepared gold nanoparticle (AuNP)-embedded collagen gels as a visible-light-responsive cell scaffold in which photoinduced single cell detachment occurs through local thermal denaturation of the collagen gel via the photothermal effe...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023522/ https://www.ncbi.nlm.nih.gov/pubmed/31952267 http://dx.doi.org/10.3390/polym12010213 |
_version_ | 1783498269182132224 |
---|---|
author | Kojima, Chie Nishio, Misaki Nakajima, Yusuke Kawano, Takeshi Takatsuka, Kenji Matsumoto, Akikazu |
author_facet | Kojima, Chie Nishio, Misaki Nakajima, Yusuke Kawano, Takeshi Takatsuka, Kenji Matsumoto, Akikazu |
author_sort | Kojima, Chie |
collection | PubMed |
description | Cell Separation is important in various biomedical fields. We have prepared gold nanoparticle (AuNP)-embedded collagen gels as a visible-light-responsive cell scaffold in which photoinduced single cell detachment occurs through local thermal denaturation of the collagen gel via the photothermal effect of AuNP. Physicochemical properties of collagen materials depend on the origin of the collagen and the presence of telopeptides. In this study, we prepared various AuNP-embedded collagen gels by using different collagen materials with and without the telopeptides to compare their thermal denaturation properties and photoinduced single cell detachment behaviors. Cellmatrix type I-C without telopeptides exhibited a lower denaturation temperature than Cellmatrix type I-A and Atelocell IAC, as examined by Fourier transform infrared (FTIR) spectroscopy, rheological analysis, and sol–gel transition observation. Three-dimensional (3D) laser microscopic imaging revealed that collagen fibers shrank in Cellmatrix type I-A upon heating, but collagen fibers disappeared in Cellmatrix type I-C upon heating. Cells cultured on the Cellmatrix type I-C-based AuNP-embedded collagen gel detached with shorter photoirradiation than on the Cellmatrix type I-A-based AuNP-embedded collagen gel, suggesting that collagen gels without telopeptides are suitable for a photoinduced single cell detachment system. |
format | Online Article Text |
id | pubmed-7023522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70235222020-03-12 Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature Kojima, Chie Nishio, Misaki Nakajima, Yusuke Kawano, Takeshi Takatsuka, Kenji Matsumoto, Akikazu Polymers (Basel) Article Cell Separation is important in various biomedical fields. We have prepared gold nanoparticle (AuNP)-embedded collagen gels as a visible-light-responsive cell scaffold in which photoinduced single cell detachment occurs through local thermal denaturation of the collagen gel via the photothermal effect of AuNP. Physicochemical properties of collagen materials depend on the origin of the collagen and the presence of telopeptides. In this study, we prepared various AuNP-embedded collagen gels by using different collagen materials with and without the telopeptides to compare their thermal denaturation properties and photoinduced single cell detachment behaviors. Cellmatrix type I-C without telopeptides exhibited a lower denaturation temperature than Cellmatrix type I-A and Atelocell IAC, as examined by Fourier transform infrared (FTIR) spectroscopy, rheological analysis, and sol–gel transition observation. Three-dimensional (3D) laser microscopic imaging revealed that collagen fibers shrank in Cellmatrix type I-A upon heating, but collagen fibers disappeared in Cellmatrix type I-C upon heating. Cells cultured on the Cellmatrix type I-C-based AuNP-embedded collagen gel detached with shorter photoirradiation than on the Cellmatrix type I-A-based AuNP-embedded collagen gel, suggesting that collagen gels without telopeptides are suitable for a photoinduced single cell detachment system. MDPI 2020-01-15 /pmc/articles/PMC7023522/ /pubmed/31952267 http://dx.doi.org/10.3390/polym12010213 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kojima, Chie Nishio, Misaki Nakajima, Yusuke Kawano, Takeshi Takatsuka, Kenji Matsumoto, Akikazu Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature |
title | Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature |
title_full | Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature |
title_fullStr | Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature |
title_full_unstemmed | Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature |
title_short | Rapid Photoinduced Single Cell Detachment from Gold Nanoparticle-Embedded Collagen Gels with Low Denaturation Temperature |
title_sort | rapid photoinduced single cell detachment from gold nanoparticle-embedded collagen gels with low denaturation temperature |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023522/ https://www.ncbi.nlm.nih.gov/pubmed/31952267 http://dx.doi.org/10.3390/polym12010213 |
work_keys_str_mv | AT kojimachie rapidphotoinducedsinglecelldetachmentfromgoldnanoparticleembeddedcollagengelswithlowdenaturationtemperature AT nishiomisaki rapidphotoinducedsinglecelldetachmentfromgoldnanoparticleembeddedcollagengelswithlowdenaturationtemperature AT nakajimayusuke rapidphotoinducedsinglecelldetachmentfromgoldnanoparticleembeddedcollagengelswithlowdenaturationtemperature AT kawanotakeshi rapidphotoinducedsinglecelldetachmentfromgoldnanoparticleembeddedcollagengelswithlowdenaturationtemperature AT takatsukakenji rapidphotoinducedsinglecelldetachmentfromgoldnanoparticleembeddedcollagengelswithlowdenaturationtemperature AT matsumotoakikazu rapidphotoinducedsinglecelldetachmentfromgoldnanoparticleembeddedcollagengelswithlowdenaturationtemperature |