Cargando…

Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering

In bone tissue engineering, an ideal scaffold is required to have favorable physical, chemical (or physicochemical), and biological (or biochemical) properties to promote osteogenesis. Although silk fibroin (SF) and/or soy protein isolate (SPI) scaffolds have been widely used as an alternative to au...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Fan, Liu, Chen, Zheng, Bowen, He, Jia, Liu, Jun, Chen, Cen, Lee, In-seop, Wang, Xiaohong, Liu, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023539/
https://www.ncbi.nlm.nih.gov/pubmed/31906498
http://dx.doi.org/10.3390/polym12010069
_version_ 1783498273106952192
author Liu, Fan
Liu, Chen
Zheng, Bowen
He, Jia
Liu, Jun
Chen, Cen
Lee, In-seop
Wang, Xiaohong
Liu, Yi
author_facet Liu, Fan
Liu, Chen
Zheng, Bowen
He, Jia
Liu, Jun
Chen, Cen
Lee, In-seop
Wang, Xiaohong
Liu, Yi
author_sort Liu, Fan
collection PubMed
description In bone tissue engineering, an ideal scaffold is required to have favorable physical, chemical (or physicochemical), and biological (or biochemical) properties to promote osteogenesis. Although silk fibroin (SF) and/or soy protein isolate (SPI) scaffolds have been widely used as an alternative to autologous and heterologous bone grafts, the poor mechanical property and insufficient osteoinductive capability has become an obstacle for their in vivo applications. Herein, β-tricalcium phosphate (β-TCP) and graphene oxide (GO) nanoparticles are incorporated into SF/SPI scaffolds simultaneously or individually. Physical and chemical properties of these composite scaffolds are evaluated using field emission scanning electron microscope (FESEM), X-ray diffraction (XRD) and attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR). Biocompatibility and osteogenesis of the composite scaffolds are evaluated using bone marrow mesenchymal stem cells (BMSCs). All the composite scaffolds have a complex porous structure with proper pore sizes and porosities. Physicochemical properties of the scaffolds can be significantly increased through the incorporation of β-TCP and GO nanoparticles. Alkaline phosphatase activity (ALP) and osteogenesis-related gene expression of the BMSCs are significantly enhanced in the presence of β-TCP and GO nanoparticles. Especially, β-TCP and GO nanoparticles have a synergistic effect on promoting osteogenesis. These results suggest that the β-TCP and GO enhanced SF/SPI scaffolds are promising candidates for bone tissue regeneration.
format Online
Article
Text
id pubmed-7023539
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70235392020-03-12 Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering Liu, Fan Liu, Chen Zheng, Bowen He, Jia Liu, Jun Chen, Cen Lee, In-seop Wang, Xiaohong Liu, Yi Polymers (Basel) Article In bone tissue engineering, an ideal scaffold is required to have favorable physical, chemical (or physicochemical), and biological (or biochemical) properties to promote osteogenesis. Although silk fibroin (SF) and/or soy protein isolate (SPI) scaffolds have been widely used as an alternative to autologous and heterologous bone grafts, the poor mechanical property and insufficient osteoinductive capability has become an obstacle for their in vivo applications. Herein, β-tricalcium phosphate (β-TCP) and graphene oxide (GO) nanoparticles are incorporated into SF/SPI scaffolds simultaneously or individually. Physical and chemical properties of these composite scaffolds are evaluated using field emission scanning electron microscope (FESEM), X-ray diffraction (XRD) and attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR). Biocompatibility and osteogenesis of the composite scaffolds are evaluated using bone marrow mesenchymal stem cells (BMSCs). All the composite scaffolds have a complex porous structure with proper pore sizes and porosities. Physicochemical properties of the scaffolds can be significantly increased through the incorporation of β-TCP and GO nanoparticles. Alkaline phosphatase activity (ALP) and osteogenesis-related gene expression of the BMSCs are significantly enhanced in the presence of β-TCP and GO nanoparticles. Especially, β-TCP and GO nanoparticles have a synergistic effect on promoting osteogenesis. These results suggest that the β-TCP and GO enhanced SF/SPI scaffolds are promising candidates for bone tissue regeneration. MDPI 2020-01-02 /pmc/articles/PMC7023539/ /pubmed/31906498 http://dx.doi.org/10.3390/polym12010069 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Fan
Liu, Chen
Zheng, Bowen
He, Jia
Liu, Jun
Chen, Cen
Lee, In-seop
Wang, Xiaohong
Liu, Yi
Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
title Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
title_full Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
title_fullStr Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
title_full_unstemmed Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
title_short Synergistic Effects on Incorporation of β-Tricalcium Phosphate and Graphene Oxide Nanoparticles to Silk Fibroin/Soy Protein Isolate Scaffolds for Bone Tissue Engineering
title_sort synergistic effects on incorporation of β-tricalcium phosphate and graphene oxide nanoparticles to silk fibroin/soy protein isolate scaffolds for bone tissue engineering
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023539/
https://www.ncbi.nlm.nih.gov/pubmed/31906498
http://dx.doi.org/10.3390/polym12010069
work_keys_str_mv AT liufan synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT liuchen synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT zhengbowen synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT hejia synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT liujun synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT chencen synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT leeinseop synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT wangxiaohong synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering
AT liuyi synergisticeffectsonincorporationofbtricalciumphosphateandgrapheneoxidenanoparticlestosilkfibroinsoyproteinisolatescaffoldsforbonetissueengineering