Cargando…
Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line
(1) Background: Inflammation molecular cues and insulin resistance development are some of the main contributors for the development and advance of the pathogenesis of inflammatory-related diseases; (2) Methods: We isolated and purified γ-conglutin protein from narrow-leafed lupin (NLL or blue lupin...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023629/ https://www.ncbi.nlm.nih.gov/pubmed/31877933 http://dx.doi.org/10.3390/antiox9010012 |
_version_ | 1783498292136509440 |
---|---|
author | Lima-Cabello, Elena Alché, Juan D. Morales-Santana, Sonia Clemente, Alfonso Jimenez-Lopez, Jose C. |
author_facet | Lima-Cabello, Elena Alché, Juan D. Morales-Santana, Sonia Clemente, Alfonso Jimenez-Lopez, Jose C. |
author_sort | Lima-Cabello, Elena |
collection | PubMed |
description | (1) Background: Inflammation molecular cues and insulin resistance development are some of the main contributors for the development and advance of the pathogenesis of inflammatory-related diseases; (2) Methods: We isolated and purified γ-conglutin protein from narrow-leafed lupin (NLL or blue lupin) mature seeds using affinity-chromatography to evaluate its anti-inflammatory activities at molecular level using both, a bacterial lipopolysaccharide (LPS)-induced inflammation and an insulin resistance pancreatic cell models; (3) Results: NLL γ-conglutin achieved a plethora of functional effects as the strong reduction of cell oxidative stress induced by inflammation through decreasing proteins carbonylation, nitric oxide synthesis and inducible nitric oxide synthase (iNOS) transcriptional levels, and raising glutathione (GSH) levels and modulation of superoxide dismutase (SOD) and catalase enzymes activities. γ-conglutin induced up-regulated transcriptomic and protein levels of insulin signalling pathway IRS-1, Glut-4, and PI3K, improving glucose uptake, while decreasing pro-inflammatory mediators as iNOs, TNFα, IL-1β, INFγ, IL-6, IL-12, IL-17, and IL-27; (4) Conclusion: These results suggest a promising use of NLL γ-conglutin protein in functional foods, which could also be implemented in alternative diagnosis and therapeutic molecular tools helping to prevent and treat inflammatory-related diseases. |
format | Online Article Text |
id | pubmed-7023629 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-70236292020-03-11 Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line Lima-Cabello, Elena Alché, Juan D. Morales-Santana, Sonia Clemente, Alfonso Jimenez-Lopez, Jose C. Antioxidants (Basel) Article (1) Background: Inflammation molecular cues and insulin resistance development are some of the main contributors for the development and advance of the pathogenesis of inflammatory-related diseases; (2) Methods: We isolated and purified γ-conglutin protein from narrow-leafed lupin (NLL or blue lupin) mature seeds using affinity-chromatography to evaluate its anti-inflammatory activities at molecular level using both, a bacterial lipopolysaccharide (LPS)-induced inflammation and an insulin resistance pancreatic cell models; (3) Results: NLL γ-conglutin achieved a plethora of functional effects as the strong reduction of cell oxidative stress induced by inflammation through decreasing proteins carbonylation, nitric oxide synthesis and inducible nitric oxide synthase (iNOS) transcriptional levels, and raising glutathione (GSH) levels and modulation of superoxide dismutase (SOD) and catalase enzymes activities. γ-conglutin induced up-regulated transcriptomic and protein levels of insulin signalling pathway IRS-1, Glut-4, and PI3K, improving glucose uptake, while decreasing pro-inflammatory mediators as iNOs, TNFα, IL-1β, INFγ, IL-6, IL-12, IL-17, and IL-27; (4) Conclusion: These results suggest a promising use of NLL γ-conglutin protein in functional foods, which could also be implemented in alternative diagnosis and therapeutic molecular tools helping to prevent and treat inflammatory-related diseases. MDPI 2019-12-23 /pmc/articles/PMC7023629/ /pubmed/31877933 http://dx.doi.org/10.3390/antiox9010012 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lima-Cabello, Elena Alché, Juan D. Morales-Santana, Sonia Clemente, Alfonso Jimenez-Lopez, Jose C. Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line |
title | Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line |
title_full | Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line |
title_fullStr | Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line |
title_full_unstemmed | Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line |
title_short | Narrow-Leafed Lupin (Lupinus angustifolius L.) Seeds Gamma-Conglutin is an Anti-Inflammatory Protein Promoting Insulin Resistance Improvement and Oxidative Stress Amelioration in PANC-1 Pancreatic Cell-Line |
title_sort | narrow-leafed lupin (lupinus angustifolius l.) seeds gamma-conglutin is an anti-inflammatory protein promoting insulin resistance improvement and oxidative stress amelioration in panc-1 pancreatic cell-line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023629/ https://www.ncbi.nlm.nih.gov/pubmed/31877933 http://dx.doi.org/10.3390/antiox9010012 |
work_keys_str_mv | AT limacabelloelena narrowleafedlupinlupinusangustifoliuslseedsgammaconglutinisanantiinflammatoryproteinpromotinginsulinresistanceimprovementandoxidativestressameliorationinpanc1pancreaticcellline AT alchejuand narrowleafedlupinlupinusangustifoliuslseedsgammaconglutinisanantiinflammatoryproteinpromotinginsulinresistanceimprovementandoxidativestressameliorationinpanc1pancreaticcellline AT moralessantanasonia narrowleafedlupinlupinusangustifoliuslseedsgammaconglutinisanantiinflammatoryproteinpromotinginsulinresistanceimprovementandoxidativestressameliorationinpanc1pancreaticcellline AT clementealfonso narrowleafedlupinlupinusangustifoliuslseedsgammaconglutinisanantiinflammatoryproteinpromotinginsulinresistanceimprovementandoxidativestressameliorationinpanc1pancreaticcellline AT jimenezlopezjosec narrowleafedlupinlupinusangustifoliuslseedsgammaconglutinisanantiinflammatoryproteinpromotinginsulinresistanceimprovementandoxidativestressameliorationinpanc1pancreaticcellline |