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Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors

BACKGROUND MBD1, the largest member of methyl binding domain family, has the most downregulated mRNA expression and upregulated methylation levels in advanced colorectal cancer (CRC). In this study, we evaluated the immune-histochemical expression of MBD1 in CRC and assessed its correlation with cli...

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Autores principales: Ghaedamini, Sholeh, Nikbakht, Mehdi, Soleimani, Mitra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Association of Gastroerterology and Hepatology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023652/
https://www.ncbi.nlm.nih.gov/pubmed/32082520
http://dx.doi.org/10.15171/mejdd.2020.162
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author Ghaedamini, Sholeh
Nikbakht, Mehdi
Soleimani, Mitra
author_facet Ghaedamini, Sholeh
Nikbakht, Mehdi
Soleimani, Mitra
author_sort Ghaedamini, Sholeh
collection PubMed
description BACKGROUND MBD1, the largest member of methyl binding domain family, has the most downregulated mRNA expression and upregulated methylation levels in advanced colorectal cancer (CRC). In this study, we evaluated the immune-histochemical expression of MBD1 in CRC and assessed its correlation with clinicopathological features to study its prognostic value in CRC. METHODS A total of 60 samples of CRC, from patients who underwent surgical gastroenterology operations, were randomly selected. The samples included one tumor-rich section per case and one adjacent tumor-free section as a normal control for that case. Then, immunohistochemistry (ICH) was performed for MBD1 protein on all samples and the expression of MBD1 was analyzed in cancerous and normal samples. In the next step, the correlation between MBD1 and clinicopathological features including age, sex, location of the tumor, grade, and stage were evaluated. RESULTS The expression of MBD1 protein had a significant downregulation in cancerous samples compared with normal control samples. This downregulation increased corresponding to both grade and stage of cancer. However, no correlation was seen between the expression of MBD1 and sex, age and location of the tumor. CONCLUSION MBD1 protein may be considered as a protein marker in the detection of CRC and its progression.
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spelling pubmed-70236522020-02-20 Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors Ghaedamini, Sholeh Nikbakht, Mehdi Soleimani, Mitra Middle East J Dig Dis Original Article BACKGROUND MBD1, the largest member of methyl binding domain family, has the most downregulated mRNA expression and upregulated methylation levels in advanced colorectal cancer (CRC). In this study, we evaluated the immune-histochemical expression of MBD1 in CRC and assessed its correlation with clinicopathological features to study its prognostic value in CRC. METHODS A total of 60 samples of CRC, from patients who underwent surgical gastroenterology operations, were randomly selected. The samples included one tumor-rich section per case and one adjacent tumor-free section as a normal control for that case. Then, immunohistochemistry (ICH) was performed for MBD1 protein on all samples and the expression of MBD1 was analyzed in cancerous and normal samples. In the next step, the correlation between MBD1 and clinicopathological features including age, sex, location of the tumor, grade, and stage were evaluated. RESULTS The expression of MBD1 protein had a significant downregulation in cancerous samples compared with normal control samples. This downregulation increased corresponding to both grade and stage of cancer. However, no correlation was seen between the expression of MBD1 and sex, age and location of the tumor. CONCLUSION MBD1 protein may be considered as a protein marker in the detection of CRC and its progression. Iranian Association of Gastroerterology and Hepatology 2020-01 /pmc/articles/PMC7023652/ /pubmed/32082520 http://dx.doi.org/10.15171/mejdd.2020.162 Text en © 2020 The Author(s) This work is published by Middle East Journal of Digestive Diseaes as an open access article distributed under the terms of the Creative Commons Attribution License https://creativecommons.org/licenses/by-nc/4.0/ Non-commercial uses of the work are permitted, provided the original work is properly cited.
spellingShingle Original Article
Ghaedamini, Sholeh
Nikbakht, Mehdi
Soleimani, Mitra
Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors
title Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors
title_full Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors
title_fullStr Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors
title_full_unstemmed Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors
title_short Assessment of Immuno-Histochemical Expression of MBD1 in Colorectal Adenocarcinoma and Its Correlations with Prognostic Factors
title_sort assessment of immuno-histochemical expression of mbd1 in colorectal adenocarcinoma and its correlations with prognostic factors
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023652/
https://www.ncbi.nlm.nih.gov/pubmed/32082520
http://dx.doi.org/10.15171/mejdd.2020.162
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