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MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2

INTRODUCTION: Recently, the incidence of melanoma has been rising and there is a lack of effective targeted therapies. The regulatory mechanisms of microRNA-1246 (miR-1246) have been found in many cancers, except melanoma. This study focused on the regulatory mechanism of miR-1246 in melanoma develo...

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Autores principales: Yu, Yanhua, Yu, Fang, Sun, Pijiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023870/
https://www.ncbi.nlm.nih.gov/pubmed/32103992
http://dx.doi.org/10.2147/OTT.S234276
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author Yu, Yanhua
Yu, Fang
Sun, Pijiang
author_facet Yu, Yanhua
Yu, Fang
Sun, Pijiang
author_sort Yu, Yanhua
collection PubMed
description INTRODUCTION: Recently, the incidence of melanoma has been rising and there is a lack of effective targeted therapies. The regulatory mechanisms of microRNA-1246 (miR-1246) have been found in many cancers, except melanoma. This study focused on the regulatory mechanism of miR-1246 in melanoma development. METHODS: The expression of miR-1246 was assessed using quantitative real-time polymerase chain reaction (RT-qPCR). Cell viability and metastasis were detected by Transwell and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays. The protein expression of epithelial mesenchymal transition (EMT) makers was assessed by Western blot analysis. The target gene of miR-1246 was detected using luciferase reporter assay. RESULTS: MiR-1246 expression was increased in melanoma tissues and cells. In addition, upregulation of miR-1246 promoted cell viability and metastasis in melanoma. Forkhead box protein A2 (FOXA2) was confirmed to be a direct target of miR-1246. And FOXA2 expression was decreased in melanoma and was suppressed by miR-1246. Importantly, upregulation of FOXA2 restored the carcinogenesis of miR-1246 in melanoma. CONCLUSION: MiR-1246 promoted cell viability and metastasis in melanoma by inhibiting FOXA2 expression.
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spelling pubmed-70238702020-02-26 MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2 Yu, Yanhua Yu, Fang Sun, Pijiang Onco Targets Ther Original Research INTRODUCTION: Recently, the incidence of melanoma has been rising and there is a lack of effective targeted therapies. The regulatory mechanisms of microRNA-1246 (miR-1246) have been found in many cancers, except melanoma. This study focused on the regulatory mechanism of miR-1246 in melanoma development. METHODS: The expression of miR-1246 was assessed using quantitative real-time polymerase chain reaction (RT-qPCR). Cell viability and metastasis were detected by Transwell and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays. The protein expression of epithelial mesenchymal transition (EMT) makers was assessed by Western blot analysis. The target gene of miR-1246 was detected using luciferase reporter assay. RESULTS: MiR-1246 expression was increased in melanoma tissues and cells. In addition, upregulation of miR-1246 promoted cell viability and metastasis in melanoma. Forkhead box protein A2 (FOXA2) was confirmed to be a direct target of miR-1246. And FOXA2 expression was decreased in melanoma and was suppressed by miR-1246. Importantly, upregulation of FOXA2 restored the carcinogenesis of miR-1246 in melanoma. CONCLUSION: MiR-1246 promoted cell viability and metastasis in melanoma by inhibiting FOXA2 expression. Dove 2020-02-11 /pmc/articles/PMC7023870/ /pubmed/32103992 http://dx.doi.org/10.2147/OTT.S234276 Text en © 2020 Yu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yu, Yanhua
Yu, Fang
Sun, Pijiang
MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2
title MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2
title_full MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2
title_fullStr MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2
title_full_unstemmed MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2
title_short MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2
title_sort microrna-1246 promotes melanoma progression through targeting foxa2
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7023870/
https://www.ncbi.nlm.nih.gov/pubmed/32103992
http://dx.doi.org/10.2147/OTT.S234276
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