Cargando…

Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS

Red yeast rice dietary supplements (RYR DS) are largely sold in Western countries for their cholesterol-lowering/regulating effect due to monacolins, mainly monacolin K (MK), which is, in fact, lovastatin, the first statin drug on the market. (1)H-NMR was used as an easy, rapid and accurate method t...

Descripción completa

Detalles Bibliográficos
Autores principales: Hachem, Rabab, Assemat, Gaëtan, Balayssac, Stéphane, Martins-Froment, Nathalie, Gilard, Véronique, Martino, Robert, Malet-Martino, Myriam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024183/
https://www.ncbi.nlm.nih.gov/pubmed/31941089
http://dx.doi.org/10.3390/molecules25020317
_version_ 1783498376020492288
author Hachem, Rabab
Assemat, Gaëtan
Balayssac, Stéphane
Martins-Froment, Nathalie
Gilard, Véronique
Martino, Robert
Malet-Martino, Myriam
author_facet Hachem, Rabab
Assemat, Gaëtan
Balayssac, Stéphane
Martins-Froment, Nathalie
Gilard, Véronique
Martino, Robert
Malet-Martino, Myriam
author_sort Hachem, Rabab
collection PubMed
description Red yeast rice dietary supplements (RYR DS) are largely sold in Western countries for their cholesterol-lowering/regulating effect due to monacolins, mainly monacolin K (MK), which is, in fact, lovastatin, the first statin drug on the market. (1)H-NMR was used as an easy, rapid and accurate method to establish the chemical profiles of 31 RYR DS and to quantify their monacolin contents. Among all the (1)H resonances of the monacolins found in RYR, only those of the ethylenic protons of the hexahydronaphthalenic ring at 5.84 and 5.56 ppm are suitable for quantification because they show no overlap with the matrix signals. The total content in monacolins per capsule or tablet determined in 28 DS (the content in 3 DS being below the limit of quantification of the method, ≈ 0.25 mg per unit dose) was close to that measured by UHPLC, as shown by the good linear correlation between the two sets of values (slope 1.00, y-intercept 0.113, r(2) 0.986). Thirteen of the 31 RYR DS analyzed (i.e., 42%) did not provide label information on the concentration of monacolins and only nine of the 18 formulations with an indication (i.e., 50%) actually contained the declared amount of monacolins.
format Online
Article
Text
id pubmed-7024183
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-70241832020-03-19 Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS Hachem, Rabab Assemat, Gaëtan Balayssac, Stéphane Martins-Froment, Nathalie Gilard, Véronique Martino, Robert Malet-Martino, Myriam Molecules Article Red yeast rice dietary supplements (RYR DS) are largely sold in Western countries for their cholesterol-lowering/regulating effect due to monacolins, mainly monacolin K (MK), which is, in fact, lovastatin, the first statin drug on the market. (1)H-NMR was used as an easy, rapid and accurate method to establish the chemical profiles of 31 RYR DS and to quantify their monacolin contents. Among all the (1)H resonances of the monacolins found in RYR, only those of the ethylenic protons of the hexahydronaphthalenic ring at 5.84 and 5.56 ppm are suitable for quantification because they show no overlap with the matrix signals. The total content in monacolins per capsule or tablet determined in 28 DS (the content in 3 DS being below the limit of quantification of the method, ≈ 0.25 mg per unit dose) was close to that measured by UHPLC, as shown by the good linear correlation between the two sets of values (slope 1.00, y-intercept 0.113, r(2) 0.986). Thirteen of the 31 RYR DS analyzed (i.e., 42%) did not provide label information on the concentration of monacolins and only nine of the 18 formulations with an indication (i.e., 50%) actually contained the declared amount of monacolins. MDPI 2020-01-13 /pmc/articles/PMC7024183/ /pubmed/31941089 http://dx.doi.org/10.3390/molecules25020317 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hachem, Rabab
Assemat, Gaëtan
Balayssac, Stéphane
Martins-Froment, Nathalie
Gilard, Véronique
Martino, Robert
Malet-Martino, Myriam
Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS
title Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS
title_full Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS
title_fullStr Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS
title_full_unstemmed Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS
title_short Comparative Chemical Profiling and Monacolins Quantification in Red Yeast Rice Dietary Supplements by (1)H-NMR and UHPLC-DAD-MS
title_sort comparative chemical profiling and monacolins quantification in red yeast rice dietary supplements by (1)h-nmr and uhplc-dad-ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024183/
https://www.ncbi.nlm.nih.gov/pubmed/31941089
http://dx.doi.org/10.3390/molecules25020317
work_keys_str_mv AT hachemrabab comparativechemicalprofilingandmonacolinsquantificationinredyeastricedietarysupplementsby1hnmranduhplcdadms
AT assematgaetan comparativechemicalprofilingandmonacolinsquantificationinredyeastricedietarysupplementsby1hnmranduhplcdadms
AT balayssacstephane comparativechemicalprofilingandmonacolinsquantificationinredyeastricedietarysupplementsby1hnmranduhplcdadms
AT martinsfromentnathalie comparativechemicalprofilingandmonacolinsquantificationinredyeastricedietarysupplementsby1hnmranduhplcdadms
AT gilardveronique comparativechemicalprofilingandmonacolinsquantificationinredyeastricedietarysupplementsby1hnmranduhplcdadms
AT martinorobert comparativechemicalprofilingandmonacolinsquantificationinredyeastricedietarysupplementsby1hnmranduhplcdadms
AT maletmartinomyriam comparativechemicalprofilingandmonacolinsquantificationinredyeastricedietarysupplementsby1hnmranduhplcdadms