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Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile

Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recov...

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Autores principales: Castro-Enríquez, Daniela D., Montaño-Leyva, Beatriz, Del Toro-Sánchez, Carmen L., Juárez-Onofre, Josué E., Carvajal-Millán, Elizabeth, López-Ahumada, Guadalupe A., Barreras-Urbina, Carlos G., Tapia-Hernández, José A., Rodríguez-Félix, Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024205/
https://www.ncbi.nlm.nih.gov/pubmed/31936683
http://dx.doi.org/10.3390/molecules25020281
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author Castro-Enríquez, Daniela D.
Montaño-Leyva, Beatriz
Del Toro-Sánchez, Carmen L.
Juárez-Onofre, Josué E.
Carvajal-Millán, Elizabeth
López-Ahumada, Guadalupe A.
Barreras-Urbina, Carlos G.
Tapia-Hernández, José A.
Rodríguez-Félix, Francisco
author_facet Castro-Enríquez, Daniela D.
Montaño-Leyva, Beatriz
Del Toro-Sánchez, Carmen L.
Juárez-Onofre, Josué E.
Carvajal-Millán, Elizabeth
López-Ahumada, Guadalupe A.
Barreras-Urbina, Carlos G.
Tapia-Hernández, José A.
Rodríguez-Félix, Francisco
author_sort Castro-Enríquez, Daniela D.
collection PubMed
description Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recovery of phytochemicals. However, its effect has not been extensively studied in extracts. Therefore, the objective of this work is to study the effect of the ultrafiltration of pitaya extract (Stenocereus thurberi) on its phytochemical content, antioxidant capacity, and identification of phenolic compounds by UPLC-DAD-MS, providing greater knowledge about the pitaya. In this study, two extracts were analyzed, the unclarified extract (UE) and the clarified extract (CE). The antioxidant capacity was higher in the CE with 15.93 ± 0.42 mM TE/g, DPPH and 18.37 ± 0.016 mM TE/g, ABTS. The UPLC-MS analysis indicated the decrease in phenolic compounds in the CE and the presence of gallic acid and resorcinol, compounds that had not been identified in other species of Stenocereus spp. The correlation analysis indicated that all the phytochemicals present in the pitaya contribute significantly to the antioxidant capacity. The ultrafiltration process could be a viable option to improve the biological activity of the natural extracts.
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spelling pubmed-70242052020-03-19 Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile Castro-Enríquez, Daniela D. Montaño-Leyva, Beatriz Del Toro-Sánchez, Carmen L. Juárez-Onofre, Josué E. Carvajal-Millán, Elizabeth López-Ahumada, Guadalupe A. Barreras-Urbina, Carlos G. Tapia-Hernández, José A. Rodríguez-Félix, Francisco Molecules Article Stenocereus thurberi is an endemic species in northwestern Mexico. It produces colorful fruits called pitayas that have an edible pulp. They have phytochemical compounds associated with biological activities. Ultrafiltration is a widely used method for the clarification of fruit juices and the recovery of phytochemicals. However, its effect has not been extensively studied in extracts. Therefore, the objective of this work is to study the effect of the ultrafiltration of pitaya extract (Stenocereus thurberi) on its phytochemical content, antioxidant capacity, and identification of phenolic compounds by UPLC-DAD-MS, providing greater knowledge about the pitaya. In this study, two extracts were analyzed, the unclarified extract (UE) and the clarified extract (CE). The antioxidant capacity was higher in the CE with 15.93 ± 0.42 mM TE/g, DPPH and 18.37 ± 0.016 mM TE/g, ABTS. The UPLC-MS analysis indicated the decrease in phenolic compounds in the CE and the presence of gallic acid and resorcinol, compounds that had not been identified in other species of Stenocereus spp. The correlation analysis indicated that all the phytochemicals present in the pitaya contribute significantly to the antioxidant capacity. The ultrafiltration process could be a viable option to improve the biological activity of the natural extracts. MDPI 2020-01-09 /pmc/articles/PMC7024205/ /pubmed/31936683 http://dx.doi.org/10.3390/molecules25020281 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Castro-Enríquez, Daniela D.
Montaño-Leyva, Beatriz
Del Toro-Sánchez, Carmen L.
Juárez-Onofre, Josué E.
Carvajal-Millán, Elizabeth
López-Ahumada, Guadalupe A.
Barreras-Urbina, Carlos G.
Tapia-Hernández, José A.
Rodríguez-Félix, Francisco
Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile
title Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile
title_full Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile
title_fullStr Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile
title_full_unstemmed Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile
title_short Effect of Ultrafiltration of Pitaya Extract (Stenocereus thurberi) on Its Phytochemical Content, Antioxidant Capacity, and UPLC-DAD-MS Profile
title_sort effect of ultrafiltration of pitaya extract (stenocereus thurberi) on its phytochemical content, antioxidant capacity, and uplc-dad-ms profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024205/
https://www.ncbi.nlm.nih.gov/pubmed/31936683
http://dx.doi.org/10.3390/molecules25020281
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