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Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus

The Nme gene/protein family of nucleoside diphosphate kinases (NDPK) was originally named after its member Nm23-H1/Nme1, the first identified metastasis suppressor. Human Nme proteins are divided in two groups. They all possess nucleoside diphosphate kinase domain (NDK). Group I (Nme1-Nme4) display...

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Autores principales: Perina, Dragutin, Korolija, Marina, Mikoč, Andreja, Halasz, Mirna, Herak Bosnar, Maja, Ćetković, Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024210/
https://www.ncbi.nlm.nih.gov/pubmed/31877804
http://dx.doi.org/10.3390/md18010013
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author Perina, Dragutin
Korolija, Marina
Mikoč, Andreja
Halasz, Mirna
Herak Bosnar, Maja
Ćetković, Helena
author_facet Perina, Dragutin
Korolija, Marina
Mikoč, Andreja
Halasz, Mirna
Herak Bosnar, Maja
Ćetković, Helena
author_sort Perina, Dragutin
collection PubMed
description The Nme gene/protein family of nucleoside diphosphate kinases (NDPK) was originally named after its member Nm23-H1/Nme1, the first identified metastasis suppressor. Human Nme proteins are divided in two groups. They all possess nucleoside diphosphate kinase domain (NDK). Group I (Nme1-Nme4) display a single type NDK domain, whereas Group II (Nme5-Nme9) display a single or several different NDK domains, associated or not associated with extra-domains. Data strongly suggest that, unlike Group I, none of the members of Group II display measurable NDPK activity, although some of them autophosphorylate. The multimeric form is required for the NDPK activity. Group I proteins are known to multimerize, while there are no data on the multimerization of Group II proteins. The Group II ancestral type protein was shown to be conserved in several species from three eukaryotic supergroups. Here, we analysed the Nme protein from an early branching eukaryotic lineage, the red alga Chondrus crispus. We show that the ancestral type protein, unlike its human homologue, was fully functional multimeric NDPK with high affinity to various types of DNA and dispersed localization throughout the eukaryotic cell. Its overexpression inhibits both cell proliferation and the anchorage-independent growth of cells in soft agar but fails to deregulate cell apoptosis. We conclude that the ancestral gene has changed during eukaryotic evolution, possibly in correlation with the protein function.
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spelling pubmed-70242102020-03-19 Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus Perina, Dragutin Korolija, Marina Mikoč, Andreja Halasz, Mirna Herak Bosnar, Maja Ćetković, Helena Mar Drugs Article The Nme gene/protein family of nucleoside diphosphate kinases (NDPK) was originally named after its member Nm23-H1/Nme1, the first identified metastasis suppressor. Human Nme proteins are divided in two groups. They all possess nucleoside diphosphate kinase domain (NDK). Group I (Nme1-Nme4) display a single type NDK domain, whereas Group II (Nme5-Nme9) display a single or several different NDK domains, associated or not associated with extra-domains. Data strongly suggest that, unlike Group I, none of the members of Group II display measurable NDPK activity, although some of them autophosphorylate. The multimeric form is required for the NDPK activity. Group I proteins are known to multimerize, while there are no data on the multimerization of Group II proteins. The Group II ancestral type protein was shown to be conserved in several species from three eukaryotic supergroups. Here, we analysed the Nme protein from an early branching eukaryotic lineage, the red alga Chondrus crispus. We show that the ancestral type protein, unlike its human homologue, was fully functional multimeric NDPK with high affinity to various types of DNA and dispersed localization throughout the eukaryotic cell. Its overexpression inhibits both cell proliferation and the anchorage-independent growth of cells in soft agar but fails to deregulate cell apoptosis. We conclude that the ancestral gene has changed during eukaryotic evolution, possibly in correlation with the protein function. MDPI 2019-12-21 /pmc/articles/PMC7024210/ /pubmed/31877804 http://dx.doi.org/10.3390/md18010013 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perina, Dragutin
Korolija, Marina
Mikoč, Andreja
Halasz, Mirna
Herak Bosnar, Maja
Ćetković, Helena
Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus
title Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus
title_full Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus
title_fullStr Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus
title_full_unstemmed Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus
title_short Characterization of Nme5-Like Gene/Protein from the Red Alga Chondrus Crispus
title_sort characterization of nme5-like gene/protein from the red alga chondrus crispus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024210/
https://www.ncbi.nlm.nih.gov/pubmed/31877804
http://dx.doi.org/10.3390/md18010013
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