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Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves

Bamboo leaves of Phyllostachys nigra (PN), Lophatherum gracile (LG), and Pleioblastus amarus (PA) are three common herbs in China. In this work, a new high performance liquid chromatography (HPLC) method for the simultaneous determination of seven compounds in bamboo leaves has been developed; and P...

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Autores principales: Ma, Ning-Hui, Guo, Jing, Xu Chen, Si-Han, Yuan, Xiu-Rong, Zhang, Tong, Ding, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024249/
https://www.ncbi.nlm.nih.gov/pubmed/31963759
http://dx.doi.org/10.3390/molecules25020409
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author Ma, Ning-Hui
Guo, Jing
Xu Chen, Si-Han
Yuan, Xiu-Rong
Zhang, Tong
Ding, Yue
author_facet Ma, Ning-Hui
Guo, Jing
Xu Chen, Si-Han
Yuan, Xiu-Rong
Zhang, Tong
Ding, Yue
author_sort Ma, Ning-Hui
collection PubMed
description Bamboo leaves of Phyllostachys nigra (PN), Lophatherum gracile (LG), and Pleioblastus amarus (PA) are three common herbs in China. In this work, a new high performance liquid chromatography (HPLC) method for the simultaneous determination of seven compounds in bamboo leaves has been developed; and PN, LG, and PA leaves were analyzed. PN showed four times as much chlorogenic acid (CA) than the other two, and contained the most isoorientin (iso-ORI) and isovitexin (iso-VIT) as well. The PA presented the most orientin (ORI) and LG covered a majority of cynaroside (CYN). We measured the antioxidant activity by scavenging the stable 2,2-diphenyl-1-pyridinohydrazinyl (DPPH) free radicals, and found that Luteolin (inhibitory concentration (IC)(50) = 0.42 µM, LUT) and CYN (IC(50) = 0.43 µM) showed 2–3 times higher antioxidant activity than iso-ORI (IC(50) = 0.81 µM), ORI (IC(50) = 0.84 µM), and other related antioxidant standards such as trolox (IC(50) = 0.97 µM) and ascorbic acid (IC(50) = 0.93 µM, VC). Among extracts, PN and PA showed considerable antioxidant activity, which was related well with the contents of CA, iso-ORI, and iso-VIT (p < 0.05). This study firstly provides evidence for functional antioxidant compounds of bamboo leaves based on statistical analysis of the HPLC analysis and DPPH assay, and it lays a foundation for its further development or utilization.
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spelling pubmed-70242492020-03-19 Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves Ma, Ning-Hui Guo, Jing Xu Chen, Si-Han Yuan, Xiu-Rong Zhang, Tong Ding, Yue Molecules Article Bamboo leaves of Phyllostachys nigra (PN), Lophatherum gracile (LG), and Pleioblastus amarus (PA) are three common herbs in China. In this work, a new high performance liquid chromatography (HPLC) method for the simultaneous determination of seven compounds in bamboo leaves has been developed; and PN, LG, and PA leaves were analyzed. PN showed four times as much chlorogenic acid (CA) than the other two, and contained the most isoorientin (iso-ORI) and isovitexin (iso-VIT) as well. The PA presented the most orientin (ORI) and LG covered a majority of cynaroside (CYN). We measured the antioxidant activity by scavenging the stable 2,2-diphenyl-1-pyridinohydrazinyl (DPPH) free radicals, and found that Luteolin (inhibitory concentration (IC)(50) = 0.42 µM, LUT) and CYN (IC(50) = 0.43 µM) showed 2–3 times higher antioxidant activity than iso-ORI (IC(50) = 0.81 µM), ORI (IC(50) = 0.84 µM), and other related antioxidant standards such as trolox (IC(50) = 0.97 µM) and ascorbic acid (IC(50) = 0.93 µM, VC). Among extracts, PN and PA showed considerable antioxidant activity, which was related well with the contents of CA, iso-ORI, and iso-VIT (p < 0.05). This study firstly provides evidence for functional antioxidant compounds of bamboo leaves based on statistical analysis of the HPLC analysis and DPPH assay, and it lays a foundation for its further development or utilization. MDPI 2020-01-18 /pmc/articles/PMC7024249/ /pubmed/31963759 http://dx.doi.org/10.3390/molecules25020409 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ma, Ning-Hui
Guo, Jing
Xu Chen, Si-Han
Yuan, Xiu-Rong
Zhang, Tong
Ding, Yue
Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves
title Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves
title_full Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves
title_fullStr Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves
title_full_unstemmed Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves
title_short Antioxidant and Compositional HPLC Analysis of Three Common Bamboo Leaves
title_sort antioxidant and compositional hplc analysis of three common bamboo leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7024249/
https://www.ncbi.nlm.nih.gov/pubmed/31963759
http://dx.doi.org/10.3390/molecules25020409
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