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Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats

OBJECTIVE: To investigate the effect of Xuan Bi Tong Yu Fang (XBTYF) on angiogenesis via the vascular endothelial growth factor- (VEGF-) Notch1/delta-like 4 (Dll4) pathway. Materials and Methods. Sixty Sprague-Dawley rats were randomly divided into six groups: control, sham-operated, myocardial isch...

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Autores principales: Li, Shuangdi, Dong, Jingrong, Ta, Guang, Liu, Yinghui, Cui, Junfeng, Li, Xiaohui, Song, Jing, Liu, Aidong, Cheng, Guangyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025065/
https://www.ncbi.nlm.nih.gov/pubmed/32089723
http://dx.doi.org/10.1155/2020/5041629
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author Li, Shuangdi
Dong, Jingrong
Ta, Guang
Liu, Yinghui
Cui, Junfeng
Li, Xiaohui
Song, Jing
Liu, Aidong
Cheng, Guangyu
author_facet Li, Shuangdi
Dong, Jingrong
Ta, Guang
Liu, Yinghui
Cui, Junfeng
Li, Xiaohui
Song, Jing
Liu, Aidong
Cheng, Guangyu
author_sort Li, Shuangdi
collection PubMed
description OBJECTIVE: To investigate the effect of Xuan Bi Tong Yu Fang (XBTYF) on angiogenesis via the vascular endothelial growth factor- (VEGF-) Notch1/delta-like 4 (Dll4) pathway. Materials and Methods. Sixty Sprague-Dawley rats were randomly divided into six groups: control, sham-operated, myocardial ischemia model, and XBTYF treatment at 3.2, 1.6, and 0.8 g/kg. Electrocardiography was performed to evaluate the successful establishment of the model. Hematoxylin-eosin staining and transmission electron microscopy were carried out to observe the morphology and mitochondrial structure in myocardial cells, respectively. TUNEL staining was performed to assess the degree of cell apoptosis. The expression of VEGF-A, Notch1, Dll4, Bcl2, Bax, caspase 3, caspase 9, and cytochrome-c (Cyt-c) was observed by western blot. RESULTS: XBTYF inhibited changes to the morphology and mitochondrial structure in cardiomyocyte and reduced cell apoptosis. Compared with the model group, XBTYF at all doses (3.2, 1.6, and 0.8 g/kg) reduced the expression of Notch1, Dll4, Bax, caspase 3, caspase 9, and Cyt-c, whereas expression of VEGF-A and Bcl2 was increased. CONCLUSION: XBTYF attenuated mitochondrial damage and cell apoptosis while promoting the angiogenesis of cardiomyocyte. The associated mechanism may be related to the VEGF-Notch1/Dll4 pathway.
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spelling pubmed-70250652020-02-21 Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats Li, Shuangdi Dong, Jingrong Ta, Guang Liu, Yinghui Cui, Junfeng Li, Xiaohui Song, Jing Liu, Aidong Cheng, Guangyu Evid Based Complement Alternat Med Research Article OBJECTIVE: To investigate the effect of Xuan Bi Tong Yu Fang (XBTYF) on angiogenesis via the vascular endothelial growth factor- (VEGF-) Notch1/delta-like 4 (Dll4) pathway. Materials and Methods. Sixty Sprague-Dawley rats were randomly divided into six groups: control, sham-operated, myocardial ischemia model, and XBTYF treatment at 3.2, 1.6, and 0.8 g/kg. Electrocardiography was performed to evaluate the successful establishment of the model. Hematoxylin-eosin staining and transmission electron microscopy were carried out to observe the morphology and mitochondrial structure in myocardial cells, respectively. TUNEL staining was performed to assess the degree of cell apoptosis. The expression of VEGF-A, Notch1, Dll4, Bcl2, Bax, caspase 3, caspase 9, and cytochrome-c (Cyt-c) was observed by western blot. RESULTS: XBTYF inhibited changes to the morphology and mitochondrial structure in cardiomyocyte and reduced cell apoptosis. Compared with the model group, XBTYF at all doses (3.2, 1.6, and 0.8 g/kg) reduced the expression of Notch1, Dll4, Bax, caspase 3, caspase 9, and Cyt-c, whereas expression of VEGF-A and Bcl2 was increased. CONCLUSION: XBTYF attenuated mitochondrial damage and cell apoptosis while promoting the angiogenesis of cardiomyocyte. The associated mechanism may be related to the VEGF-Notch1/Dll4 pathway. Hindawi 2020-02-05 /pmc/articles/PMC7025065/ /pubmed/32089723 http://dx.doi.org/10.1155/2020/5041629 Text en Copyright © 2020 Shuangdi Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Shuangdi
Dong, Jingrong
Ta, Guang
Liu, Yinghui
Cui, Junfeng
Li, Xiaohui
Song, Jing
Liu, Aidong
Cheng, Guangyu
Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats
title Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats
title_full Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats
title_fullStr Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats
title_full_unstemmed Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats
title_short Xuan Bi Tong Yu Fang Promotes Angiogenesis via VEGF-Notch1/Dll4 Pathway in Myocardial Ischemic Rats
title_sort xuan bi tong yu fang promotes angiogenesis via vegf-notch1/dll4 pathway in myocardial ischemic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025065/
https://www.ncbi.nlm.nih.gov/pubmed/32089723
http://dx.doi.org/10.1155/2020/5041629
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