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Persistent and polarised global actin flow is essential for directionality during cell migration

Cell migration is hypothesised to involve a cycle of behaviours beginning with leading edge extension. However, recent evidence suggests that the leading edge may be dispensable for migration, raising the question of what actually controls cell directionality. Here we exploit the embryonic migration...

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Autores principales: Yolland, Lawrence, Burki, Mubarik, Marcotti, Stefania, Luchici, Andrei, Kenny, Fiona N., Davis, John Robert, Serna-Morales, Eduardo, Müller, Jan, Sixt, Michael, Davidson, Andrew, Wood, Will, Schumacher, Linus J., Endres, Robert G., Miodownik, Mark, Stramer, Brian M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025891/
https://www.ncbi.nlm.nih.gov/pubmed/31685997
http://dx.doi.org/10.1038/s41556-019-0411-5
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author Yolland, Lawrence
Burki, Mubarik
Marcotti, Stefania
Luchici, Andrei
Kenny, Fiona N.
Davis, John Robert
Serna-Morales, Eduardo
Müller, Jan
Sixt, Michael
Davidson, Andrew
Wood, Will
Schumacher, Linus J.
Endres, Robert G.
Miodownik, Mark
Stramer, Brian M.
author_facet Yolland, Lawrence
Burki, Mubarik
Marcotti, Stefania
Luchici, Andrei
Kenny, Fiona N.
Davis, John Robert
Serna-Morales, Eduardo
Müller, Jan
Sixt, Michael
Davidson, Andrew
Wood, Will
Schumacher, Linus J.
Endres, Robert G.
Miodownik, Mark
Stramer, Brian M.
author_sort Yolland, Lawrence
collection PubMed
description Cell migration is hypothesised to involve a cycle of behaviours beginning with leading edge extension. However, recent evidence suggests that the leading edge may be dispensable for migration, raising the question of what actually controls cell directionality. Here we exploit the embryonic migration of Drosophila macrophages to bridge the different temporal scales of the behaviours controlling motility. This reveals that edge fluctuations during random motility are impersistent and weakly correlated with motion. In contrast, flow of the actin network behind the leading edge is highly persistent. Quantification of actin flow structure during migration reveals a stable organisation and asymmetry in the cell-wide flowfield that strongly correlates with cell directionality. This organisation is regulated by a gradient of actin network compression and destruction, which is controlled by myosin contraction and cofilin-mediated disassembly. It is this stable actin-flow polarity, which integrates rapid fluctuations of the leading edge that controls inherent cellular persistence.
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spelling pubmed-70258912020-05-04 Persistent and polarised global actin flow is essential for directionality during cell migration Yolland, Lawrence Burki, Mubarik Marcotti, Stefania Luchici, Andrei Kenny, Fiona N. Davis, John Robert Serna-Morales, Eduardo Müller, Jan Sixt, Michael Davidson, Andrew Wood, Will Schumacher, Linus J. Endres, Robert G. Miodownik, Mark Stramer, Brian M. Nat Cell Biol Article Cell migration is hypothesised to involve a cycle of behaviours beginning with leading edge extension. However, recent evidence suggests that the leading edge may be dispensable for migration, raising the question of what actually controls cell directionality. Here we exploit the embryonic migration of Drosophila macrophages to bridge the different temporal scales of the behaviours controlling motility. This reveals that edge fluctuations during random motility are impersistent and weakly correlated with motion. In contrast, flow of the actin network behind the leading edge is highly persistent. Quantification of actin flow structure during migration reveals a stable organisation and asymmetry in the cell-wide flowfield that strongly correlates with cell directionality. This organisation is regulated by a gradient of actin network compression and destruction, which is controlled by myosin contraction and cofilin-mediated disassembly. It is this stable actin-flow polarity, which integrates rapid fluctuations of the leading edge that controls inherent cellular persistence. 2019-11-04 2019-11 /pmc/articles/PMC7025891/ /pubmed/31685997 http://dx.doi.org/10.1038/s41556-019-0411-5 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Yolland, Lawrence
Burki, Mubarik
Marcotti, Stefania
Luchici, Andrei
Kenny, Fiona N.
Davis, John Robert
Serna-Morales, Eduardo
Müller, Jan
Sixt, Michael
Davidson, Andrew
Wood, Will
Schumacher, Linus J.
Endres, Robert G.
Miodownik, Mark
Stramer, Brian M.
Persistent and polarised global actin flow is essential for directionality during cell migration
title Persistent and polarised global actin flow is essential for directionality during cell migration
title_full Persistent and polarised global actin flow is essential for directionality during cell migration
title_fullStr Persistent and polarised global actin flow is essential for directionality during cell migration
title_full_unstemmed Persistent and polarised global actin flow is essential for directionality during cell migration
title_short Persistent and polarised global actin flow is essential for directionality during cell migration
title_sort persistent and polarised global actin flow is essential for directionality during cell migration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7025891/
https://www.ncbi.nlm.nih.gov/pubmed/31685997
http://dx.doi.org/10.1038/s41556-019-0411-5
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