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Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells

An inappropriate diet, particularly excessive consumption of dietary fats and oils, may have a major negative impact on beta-cell function and cause type 2 diabetes mellitus. To investigate this issue, we examined the toxicity of free fatty acid (FFA) compositions mirroring the FFA profiles of vario...

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Autores principales: von Hanstein, Anna-Sophie, Lenzen, Sigurd, Plötz, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026177/
https://www.ncbi.nlm.nih.gov/pubmed/32066652
http://dx.doi.org/10.1038/s41387-020-0108-7
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author von Hanstein, Anna-Sophie
Lenzen, Sigurd
Plötz, Thomas
author_facet von Hanstein, Anna-Sophie
Lenzen, Sigurd
Plötz, Thomas
author_sort von Hanstein, Anna-Sophie
collection PubMed
description An inappropriate diet, particularly excessive consumption of dietary fats and oils, may have a major negative impact on beta-cell function and cause type 2 diabetes mellitus. To investigate this issue, we examined the toxicity of free fatty acid (FFA) compositions mirroring the FFA profiles of various popular edible oils in human EndoC-βH1 beta-cells and in rat islets. For this purpose, we made compositions consisting exclusively of various FFAs in different volumetric percentages mimicking these oils and additionally mixtures of these compositions. Human EndoC-βH1 beta-cells were incubated with different oil compositions and the toxicity, lipid droplet formation, ER-stress, and H(2)O(2) production were analyzed. Compositions with prominent content of saturated as well as unsaturated long-chain FFAs showed moderate but significant toxicity both in human EndoC-βH1 beta-cells and rat islets, however, without further measurable metabolic impairments. On the other hand compositions with high content of medium-chain FFAs revealed no toxicity. A composition with 50% of the very long-chain unsaturated FFA erucic acid caused high toxicity with concomitant peroxisomal H(2)O(2) production. The toxicity of FFAs to human EndoC-βH1 beta-cells was dampened in mixtures of FFA compositions with a significant content of medium-chain FFAs, but not with a significant proportion of unsaturated FFAs.
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spelling pubmed-70261772020-03-03 Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells von Hanstein, Anna-Sophie Lenzen, Sigurd Plötz, Thomas Nutr Diabetes Brief Communication An inappropriate diet, particularly excessive consumption of dietary fats and oils, may have a major negative impact on beta-cell function and cause type 2 diabetes mellitus. To investigate this issue, we examined the toxicity of free fatty acid (FFA) compositions mirroring the FFA profiles of various popular edible oils in human EndoC-βH1 beta-cells and in rat islets. For this purpose, we made compositions consisting exclusively of various FFAs in different volumetric percentages mimicking these oils and additionally mixtures of these compositions. Human EndoC-βH1 beta-cells were incubated with different oil compositions and the toxicity, lipid droplet formation, ER-stress, and H(2)O(2) production were analyzed. Compositions with prominent content of saturated as well as unsaturated long-chain FFAs showed moderate but significant toxicity both in human EndoC-βH1 beta-cells and rat islets, however, without further measurable metabolic impairments. On the other hand compositions with high content of medium-chain FFAs revealed no toxicity. A composition with 50% of the very long-chain unsaturated FFA erucic acid caused high toxicity with concomitant peroxisomal H(2)O(2) production. The toxicity of FFAs to human EndoC-βH1 beta-cells was dampened in mixtures of FFA compositions with a significant content of medium-chain FFAs, but not with a significant proportion of unsaturated FFAs. Nature Publishing Group UK 2020-01-27 /pmc/articles/PMC7026177/ /pubmed/32066652 http://dx.doi.org/10.1038/s41387-020-0108-7 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Brief Communication
von Hanstein, Anna-Sophie
Lenzen, Sigurd
Plötz, Thomas
Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells
title Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells
title_full Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells
title_fullStr Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells
title_full_unstemmed Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells
title_short Toxicity of fatty acid profiles of popular edible oils in human EndoC-βH1 beta-cells
title_sort toxicity of fatty acid profiles of popular edible oils in human endoc-βh1 beta-cells
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026177/
https://www.ncbi.nlm.nih.gov/pubmed/32066652
http://dx.doi.org/10.1038/s41387-020-0108-7
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