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Strategies for in vitro engineering of the translation machinery
Engineering the process of molecular translation, or protein biosynthesis, has emerged as a major opportunity in synthetic and chemical biology to generate novel biological insights and enable new applications (e.g. designer protein therapeutics). Here, we review methods for engineering the process...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026604/ https://www.ncbi.nlm.nih.gov/pubmed/31777928 http://dx.doi.org/10.1093/nar/gkz1011 |
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author | Hammerling, Michael J Krüger, Antje Jewett, Michael C |
author_facet | Hammerling, Michael J Krüger, Antje Jewett, Michael C |
author_sort | Hammerling, Michael J |
collection | PubMed |
description | Engineering the process of molecular translation, or protein biosynthesis, has emerged as a major opportunity in synthetic and chemical biology to generate novel biological insights and enable new applications (e.g. designer protein therapeutics). Here, we review methods for engineering the process of translation in vitro. We discuss the advantages and drawbacks of the two major strategies—purified and extract-based systems—and how they may be used to manipulate and study translation. Techniques to engineer each component of the translation machinery are covered in turn, including transfer RNAs, translation factors, and the ribosome. Finally, future directions and enabling technological advances for the field are discussed. |
format | Online Article Text |
id | pubmed-7026604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-70266042020-02-25 Strategies for in vitro engineering of the translation machinery Hammerling, Michael J Krüger, Antje Jewett, Michael C Nucleic Acids Res Survey and Summary Engineering the process of molecular translation, or protein biosynthesis, has emerged as a major opportunity in synthetic and chemical biology to generate novel biological insights and enable new applications (e.g. designer protein therapeutics). Here, we review methods for engineering the process of translation in vitro. We discuss the advantages and drawbacks of the two major strategies—purified and extract-based systems—and how they may be used to manipulate and study translation. Techniques to engineer each component of the translation machinery are covered in turn, including transfer RNAs, translation factors, and the ribosome. Finally, future directions and enabling technological advances for the field are discussed. Oxford University Press 2020-02-20 2019-11-28 /pmc/articles/PMC7026604/ /pubmed/31777928 http://dx.doi.org/10.1093/nar/gkz1011 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Survey and Summary Hammerling, Michael J Krüger, Antje Jewett, Michael C Strategies for in vitro engineering of the translation machinery |
title | Strategies for in vitro engineering of the translation machinery |
title_full | Strategies for in vitro engineering of the translation machinery |
title_fullStr | Strategies for in vitro engineering of the translation machinery |
title_full_unstemmed | Strategies for in vitro engineering of the translation machinery |
title_short | Strategies for in vitro engineering of the translation machinery |
title_sort | strategies for in vitro engineering of the translation machinery |
topic | Survey and Summary |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026604/ https://www.ncbi.nlm.nih.gov/pubmed/31777928 http://dx.doi.org/10.1093/nar/gkz1011 |
work_keys_str_mv | AT hammerlingmichaelj strategiesforinvitroengineeringofthetranslationmachinery AT krugerantje strategiesforinvitroengineeringofthetranslationmachinery AT jewettmichaelc strategiesforinvitroengineeringofthetranslationmachinery |