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Ribosome pausing, a dangerous necessity for co-translational events

In recent years translation elongation has emerged as an important contributor to the regulation of gene expression. There are multiple quality control checkpoints along the way of producing mature proteins and targeting them to the right cellular compartment, or associating them correctly with thei...

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Autores principales: Collart, Martine A, Weiss, Benjamin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026645/
https://www.ncbi.nlm.nih.gov/pubmed/31598688
http://dx.doi.org/10.1093/nar/gkz763
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author Collart, Martine A
Weiss, Benjamin
author_facet Collart, Martine A
Weiss, Benjamin
author_sort Collart, Martine A
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description In recent years translation elongation has emerged as an important contributor to the regulation of gene expression. There are multiple quality control checkpoints along the way of producing mature proteins and targeting them to the right cellular compartment, or associating them correctly with their partners. Ribosomes pause to allow co-translational protein folding, protein targeting or protein interactions, and the pausing is dictated by a combination of the mRNA sequence and structure, the tRNA availability and the nascent peptide. However, ribosome pausing can also lead to ribosome collisions and co-translational degradation of both mRNA and nascent chain. Understanding how the translating ribosome tunes the different maturation steps that nascent proteins must undergo, what the timing of these maturation events is, and how degradation can be avoided when pausing is needed, is now possible by the emergence of methods to follow ribosome dynamics in vivo. This review summarizes some of the recent studies that have advanced our knowledge about co-translational events using the power of ribosome profiling, and some of the questions that have emerged from these studies.
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spelling pubmed-70266452020-02-25 Ribosome pausing, a dangerous necessity for co-translational events Collart, Martine A Weiss, Benjamin Nucleic Acids Res Survey and Summary In recent years translation elongation has emerged as an important contributor to the regulation of gene expression. There are multiple quality control checkpoints along the way of producing mature proteins and targeting them to the right cellular compartment, or associating them correctly with their partners. Ribosomes pause to allow co-translational protein folding, protein targeting or protein interactions, and the pausing is dictated by a combination of the mRNA sequence and structure, the tRNA availability and the nascent peptide. However, ribosome pausing can also lead to ribosome collisions and co-translational degradation of both mRNA and nascent chain. Understanding how the translating ribosome tunes the different maturation steps that nascent proteins must undergo, what the timing of these maturation events is, and how degradation can be avoided when pausing is needed, is now possible by the emergence of methods to follow ribosome dynamics in vivo. This review summarizes some of the recent studies that have advanced our knowledge about co-translational events using the power of ribosome profiling, and some of the questions that have emerged from these studies. Oxford University Press 2020-02-20 2019-09-06 /pmc/articles/PMC7026645/ /pubmed/31598688 http://dx.doi.org/10.1093/nar/gkz763 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Survey and Summary
Collart, Martine A
Weiss, Benjamin
Ribosome pausing, a dangerous necessity for co-translational events
title Ribosome pausing, a dangerous necessity for co-translational events
title_full Ribosome pausing, a dangerous necessity for co-translational events
title_fullStr Ribosome pausing, a dangerous necessity for co-translational events
title_full_unstemmed Ribosome pausing, a dangerous necessity for co-translational events
title_short Ribosome pausing, a dangerous necessity for co-translational events
title_sort ribosome pausing, a dangerous necessity for co-translational events
topic Survey and Summary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026645/
https://www.ncbi.nlm.nih.gov/pubmed/31598688
http://dx.doi.org/10.1093/nar/gkz763
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