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A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons

The histone demethylase LSD1 is a key chromatin regulator that is often deregulated in cancer. Its ortholog, dLsd1 plays a crucial role in Drosophila oogenesis; however, our knowledge of dLsd1 function is insufficient to explain its role in the ovary. Here, we have performed genome-wide analysis of...

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Autores principales: Lepesant, Julie M J, Iampietro, Carole, Galeota, Eugenia, Augé, Benoit, Aguirrenbengoa, Marion, Mercé, Clemèntine, Chaubet, Camille, Rocher, Vincent, Haenlin, Marc, Waltzer, Lucas, Pelizzola, Mattia, Di Stefano, Luisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026653/
https://www.ncbi.nlm.nih.gov/pubmed/31799607
http://dx.doi.org/10.1093/nar/gkz1142
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author Lepesant, Julie M J
Iampietro, Carole
Galeota, Eugenia
Augé, Benoit
Aguirrenbengoa, Marion
Mercé, Clemèntine
Chaubet, Camille
Rocher, Vincent
Haenlin, Marc
Waltzer, Lucas
Pelizzola, Mattia
Di Stefano, Luisa
author_facet Lepesant, Julie M J
Iampietro, Carole
Galeota, Eugenia
Augé, Benoit
Aguirrenbengoa, Marion
Mercé, Clemèntine
Chaubet, Camille
Rocher, Vincent
Haenlin, Marc
Waltzer, Lucas
Pelizzola, Mattia
Di Stefano, Luisa
author_sort Lepesant, Julie M J
collection PubMed
description The histone demethylase LSD1 is a key chromatin regulator that is often deregulated in cancer. Its ortholog, dLsd1 plays a crucial role in Drosophila oogenesis; however, our knowledge of dLsd1 function is insufficient to explain its role in the ovary. Here, we have performed genome-wide analysis of dLsd1 binding in the ovary, and we document that dLsd1 is preferentially associated to the transcription start site of developmental genes. We uncovered an unanticipated interplay between dLsd1 and the GATA transcription factor Serpent and we report an unexpected role for Serpent in oogenesis. Besides, our transcriptomic data show that reducing dLsd1 levels results in ectopic transposable elements (TE) expression correlated with changes in H3K4me2 and H3K9me2 at TE loci. In addition, our results suggest that dLsd1 is required for Piwi dependent TE silencing. Hence, we propose that dLsd1 plays crucial roles in establishing specific gene expression programs and in repressing transposons during oogenesis.
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spelling pubmed-70266532020-02-25 A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons Lepesant, Julie M J Iampietro, Carole Galeota, Eugenia Augé, Benoit Aguirrenbengoa, Marion Mercé, Clemèntine Chaubet, Camille Rocher, Vincent Haenlin, Marc Waltzer, Lucas Pelizzola, Mattia Di Stefano, Luisa Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The histone demethylase LSD1 is a key chromatin regulator that is often deregulated in cancer. Its ortholog, dLsd1 plays a crucial role in Drosophila oogenesis; however, our knowledge of dLsd1 function is insufficient to explain its role in the ovary. Here, we have performed genome-wide analysis of dLsd1 binding in the ovary, and we document that dLsd1 is preferentially associated to the transcription start site of developmental genes. We uncovered an unanticipated interplay between dLsd1 and the GATA transcription factor Serpent and we report an unexpected role for Serpent in oogenesis. Besides, our transcriptomic data show that reducing dLsd1 levels results in ectopic transposable elements (TE) expression correlated with changes in H3K4me2 and H3K9me2 at TE loci. In addition, our results suggest that dLsd1 is required for Piwi dependent TE silencing. Hence, we propose that dLsd1 plays crucial roles in establishing specific gene expression programs and in repressing transposons during oogenesis. Oxford University Press 2020-02-20 2019-12-04 /pmc/articles/PMC7026653/ /pubmed/31799607 http://dx.doi.org/10.1093/nar/gkz1142 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene regulation, Chromatin and Epigenetics
Lepesant, Julie M J
Iampietro, Carole
Galeota, Eugenia
Augé, Benoit
Aguirrenbengoa, Marion
Mercé, Clemèntine
Chaubet, Camille
Rocher, Vincent
Haenlin, Marc
Waltzer, Lucas
Pelizzola, Mattia
Di Stefano, Luisa
A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons
title A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons
title_full A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons
title_fullStr A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons
title_full_unstemmed A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons
title_short A dual role of dLsd1 in oogenesis: regulating developmental genes and repressing transposons
title_sort dual role of dlsd1 in oogenesis: regulating developmental genes and repressing transposons
topic Gene regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026653/
https://www.ncbi.nlm.nih.gov/pubmed/31799607
http://dx.doi.org/10.1093/nar/gkz1142
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