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Comparison of the performance of an amplicon sequencing assay based on Oxford Nanopore technology to real-time PCR assays for detecting bacterial biodefense pathogens
BACKGROUND: The state-of-the-art in nucleic acid based biodetection continues to be polymerase chain reaction (PCR), and many real-time PCR assays targeting biodefense pathogens for biosurveillance are in widespread use. These assays are predominantly singleplex; i.e. one assay tests for the presenc...
Autores principales: | Player, Robert, Verratti, Kathleen, Staab, Andrea, Bradburne, Christopher, Grady, Sarah, Goodwin, Bruce, Sozhamannan, Shanmuga |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7026984/ https://www.ncbi.nlm.nih.gov/pubmed/32066372 http://dx.doi.org/10.1186/s12864-020-6557-5 |
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