Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model

The present study was designed to investigate the effect of adipose-derived stem cells (ADSCs) on acute graft vs. host disease (aGVHD) and hematopoietic recovery after allogeneic hematopoietic stem cell transplantation. ADSCs, bone marrow-derived stem cells (BMSCs) and fibroblasts were cultured. ADS...

Descripción completa

Detalles Bibliográficos
Autores principales: Jiang, Ming, Bi, Xiaojuan, Duan, Xianlin, Pang, Nannan, Wang, Hongbo, Yuan, Hailong, Zhang, Rongyao, Cui, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027076/
https://www.ncbi.nlm.nih.gov/pubmed/32104226
http://dx.doi.org/10.3892/etm.2020.8430
_version_ 1783498794276487168
author Jiang, Ming
Bi, Xiaojuan
Duan, Xianlin
Pang, Nannan
Wang, Hongbo
Yuan, Hailong
Zhang, Rongyao
Cui, Lei
author_facet Jiang, Ming
Bi, Xiaojuan
Duan, Xianlin
Pang, Nannan
Wang, Hongbo
Yuan, Hailong
Zhang, Rongyao
Cui, Lei
author_sort Jiang, Ming
collection PubMed
description The present study was designed to investigate the effect of adipose-derived stem cells (ADSCs) on acute graft vs. host disease (aGVHD) and hematopoietic recovery after allogeneic hematopoietic stem cell transplantation. ADSCs, bone marrow-derived stem cells (BMSCs) and fibroblasts were cultured. ADSCs were cocultured with hematopoietic stem/progenitor cells. Then, ADSCs were infused into the aGVHD rat model. The survival of the rats was recorded. Livers and small intestines were obtained from sacrificed rats for pathological examinations. Expression of the Sry gene in recipient rats that survived longer than 21 days was examined by real-time PCR to detect the presence of donor Y chromosome. Expression of serum interferon (INF)-γ and interleukin (IL)-4 was detected by ELISA at 0, 7, 14, 21 and 50 days after transplantation. Transplantation of ADSCs improved the survival of aGVHD rats. Survived ADSCs participated in hematopoietic reconstitution in aGVHD rats. ADSCs decreased aGVHD severity by immunomodulation. ADSCs support the proliferation of hematopoietic stem/progenitor cells in vitro. The present study demonstrated that ADSCs may reduce aGVHD by influencing the balance of IL-4 and INF-γ and can promote long-term hematopoiesis.
format Online
Article
Text
id pubmed-7027076
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-70270762020-02-26 Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model Jiang, Ming Bi, Xiaojuan Duan, Xianlin Pang, Nannan Wang, Hongbo Yuan, Hailong Zhang, Rongyao Cui, Lei Exp Ther Med Articles The present study was designed to investigate the effect of adipose-derived stem cells (ADSCs) on acute graft vs. host disease (aGVHD) and hematopoietic recovery after allogeneic hematopoietic stem cell transplantation. ADSCs, bone marrow-derived stem cells (BMSCs) and fibroblasts were cultured. ADSCs were cocultured with hematopoietic stem/progenitor cells. Then, ADSCs were infused into the aGVHD rat model. The survival of the rats was recorded. Livers and small intestines were obtained from sacrificed rats for pathological examinations. Expression of the Sry gene in recipient rats that survived longer than 21 days was examined by real-time PCR to detect the presence of donor Y chromosome. Expression of serum interferon (INF)-γ and interleukin (IL)-4 was detected by ELISA at 0, 7, 14, 21 and 50 days after transplantation. Transplantation of ADSCs improved the survival of aGVHD rats. Survived ADSCs participated in hematopoietic reconstitution in aGVHD rats. ADSCs decreased aGVHD severity by immunomodulation. ADSCs support the proliferation of hematopoietic stem/progenitor cells in vitro. The present study demonstrated that ADSCs may reduce aGVHD by influencing the balance of IL-4 and INF-γ and can promote long-term hematopoiesis. D.A. Spandidos 2020-03 2020-01-07 /pmc/articles/PMC7027076/ /pubmed/32104226 http://dx.doi.org/10.3892/etm.2020.8430 Text en Copyright: © Jiang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Jiang, Ming
Bi, Xiaojuan
Duan, Xianlin
Pang, Nannan
Wang, Hongbo
Yuan, Hailong
Zhang, Rongyao
Cui, Lei
Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model
title Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model
title_full Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model
title_fullStr Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model
title_full_unstemmed Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model
title_short Adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the aGVHD model
title_sort adipose tissue-derived stem cells modulate immune function in vivo and promote long-term hematopoiesis in vitro using the agvhd model
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027076/
https://www.ncbi.nlm.nih.gov/pubmed/32104226
http://dx.doi.org/10.3892/etm.2020.8430
work_keys_str_mv AT jiangming adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel
AT bixiaojuan adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel
AT duanxianlin adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel
AT pangnannan adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel
AT wanghongbo adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel
AT yuanhailong adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel
AT zhangrongyao adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel
AT cuilei adiposetissuederivedstemcellsmodulateimmunefunctioninvivoandpromotelongtermhematopoiesisinvitrousingtheagvhdmodel

Ejemplares similares