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Cytostatic effects of plant essential oils on human skin and lung cells

Essential oils are volatile compounds extracted from various plants by distillation, hydrodiffusion or compression. In recent years, the use of essential oils has gained popularity. Many pharmaceutical, cosmetic, sanitary, food industry and agriculture studies have revealed that essential oils exert...

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Autores principales: Ahn, Changhwan, Lee, Jae-Hwan, Park, Mi-Jin, Kim, Jae-Woo, Yang, Jiyoon, Yoo, Yeong-Min, Jeung, Eui-Bae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027107/
https://www.ncbi.nlm.nih.gov/pubmed/32104260
http://dx.doi.org/10.3892/etm.2020.8460
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author Ahn, Changhwan
Lee, Jae-Hwan
Park, Mi-Jin
Kim, Jae-Woo
Yang, Jiyoon
Yoo, Yeong-Min
Jeung, Eui-Bae
author_facet Ahn, Changhwan
Lee, Jae-Hwan
Park, Mi-Jin
Kim, Jae-Woo
Yang, Jiyoon
Yoo, Yeong-Min
Jeung, Eui-Bae
author_sort Ahn, Changhwan
collection PubMed
description Essential oils are volatile compounds extracted from various plants by distillation, hydrodiffusion or compression. In recent years, the use of essential oils has gained popularity. Many pharmaceutical, cosmetic, sanitary, food industry and agriculture studies have revealed that essential oils exert antibacterial, antiviral, antifungal, antiparasitic, insecticidal, anticancer, neuroprotective, psychophysiological and anti-aging effects. Despite their reported uses, recent studies of eukaryotic cells have demonstrated that essential oils exert prooxidant and cytotoxic effects. Therefore, for the effective clinical use of essential oils, an evaluation of their cytotoxicity and the identification of the mechanisms affecting cell viability are required. To evaluate cytotoxicity, the present study determined the IC(50) values of 15 essential oils provided by the Korea Forest Research Institute (Pinus densiflora for. multicaulis Uyeki, Trifolium repens, Ligularia fischeri, Abies nephrolepis, Illicium anisatum, Zanthoxylum coreanum, Abies koreana, Lindera obtusiloba, Chamaecyparis obtuse, Pinus densiflora, Magnolia kobus, Picea koraiensis, Picea abies, Abies holophylla and Platycladus orientalis). Their effect was then assessed in human lung cells (A549) and human skin cells (Detroit 551) by performing cell counting kit-8 assays. To identify the mechanism associated with each oil's cytotoxicity, expressions of cytotoxicity-associated marker genes (cyclin A, cyclin B, cyclin D and cyclin E) involved in the cell cycle and caspase-3 (involved in cell death) were examined by performing reverse transcription-quantitative PCR and western blotting. In conclusion, plant essential oils can be used as a good source of medicine. However, without examining the safety of essential oils, they cannot be used in clinics. The results included estimates of the degree of cytotoxicity and the mechanism of cell death for each oil. It is expected that the data obtained from the current study will form guidelines for the clinically appropriate and safe use of these tested essential oils.
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spelling pubmed-70271072020-02-26 Cytostatic effects of plant essential oils on human skin and lung cells Ahn, Changhwan Lee, Jae-Hwan Park, Mi-Jin Kim, Jae-Woo Yang, Jiyoon Yoo, Yeong-Min Jeung, Eui-Bae Exp Ther Med Articles Essential oils are volatile compounds extracted from various plants by distillation, hydrodiffusion or compression. In recent years, the use of essential oils has gained popularity. Many pharmaceutical, cosmetic, sanitary, food industry and agriculture studies have revealed that essential oils exert antibacterial, antiviral, antifungal, antiparasitic, insecticidal, anticancer, neuroprotective, psychophysiological and anti-aging effects. Despite their reported uses, recent studies of eukaryotic cells have demonstrated that essential oils exert prooxidant and cytotoxic effects. Therefore, for the effective clinical use of essential oils, an evaluation of their cytotoxicity and the identification of the mechanisms affecting cell viability are required. To evaluate cytotoxicity, the present study determined the IC(50) values of 15 essential oils provided by the Korea Forest Research Institute (Pinus densiflora for. multicaulis Uyeki, Trifolium repens, Ligularia fischeri, Abies nephrolepis, Illicium anisatum, Zanthoxylum coreanum, Abies koreana, Lindera obtusiloba, Chamaecyparis obtuse, Pinus densiflora, Magnolia kobus, Picea koraiensis, Picea abies, Abies holophylla and Platycladus orientalis). Their effect was then assessed in human lung cells (A549) and human skin cells (Detroit 551) by performing cell counting kit-8 assays. To identify the mechanism associated with each oil's cytotoxicity, expressions of cytotoxicity-associated marker genes (cyclin A, cyclin B, cyclin D and cyclin E) involved in the cell cycle and caspase-3 (involved in cell death) were examined by performing reverse transcription-quantitative PCR and western blotting. In conclusion, plant essential oils can be used as a good source of medicine. However, without examining the safety of essential oils, they cannot be used in clinics. The results included estimates of the degree of cytotoxicity and the mechanism of cell death for each oil. It is expected that the data obtained from the current study will form guidelines for the clinically appropriate and safe use of these tested essential oils. D.A. Spandidos 2020-03 2020-01-17 /pmc/articles/PMC7027107/ /pubmed/32104260 http://dx.doi.org/10.3892/etm.2020.8460 Text en Copyright: © Ahn et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ahn, Changhwan
Lee, Jae-Hwan
Park, Mi-Jin
Kim, Jae-Woo
Yang, Jiyoon
Yoo, Yeong-Min
Jeung, Eui-Bae
Cytostatic effects of plant essential oils on human skin and lung cells
title Cytostatic effects of plant essential oils on human skin and lung cells
title_full Cytostatic effects of plant essential oils on human skin and lung cells
title_fullStr Cytostatic effects of plant essential oils on human skin and lung cells
title_full_unstemmed Cytostatic effects of plant essential oils on human skin and lung cells
title_short Cytostatic effects of plant essential oils on human skin and lung cells
title_sort cytostatic effects of plant essential oils on human skin and lung cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027107/
https://www.ncbi.nlm.nih.gov/pubmed/32104260
http://dx.doi.org/10.3892/etm.2020.8460
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