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Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells

Proliferative vitreoretinopathy (PVR) is characterised by the contraction and growth of fibrotic membranes on the retina and within the vitreous body. Retinal pigment epithelial (RPE) cells, a major cellular component of the fibrotic membrane, is one of the cell types that have been previously repor...

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Autores principales: Qin, Dong, Jin, Xuemin, Jiang, Yanrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027231/
https://www.ncbi.nlm.nih.gov/pubmed/32104303
http://dx.doi.org/10.3892/etm.2020.8463
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author Qin, Dong
Jin, Xuemin
Jiang, Yanrong
author_facet Qin, Dong
Jin, Xuemin
Jiang, Yanrong
author_sort Qin, Dong
collection PubMed
description Proliferative vitreoretinopathy (PVR) is characterised by the contraction and growth of fibrotic membranes on the retina and within the vitreous body. Retinal pigment epithelial (RPE) cells, a major cellular component of the fibrotic membrane, is one of the cell types that have been previously reported to associate with PVR pathogenesis. During PVR, RPE cells undergo increased cell proliferation, migration and the secretion of extracellular matrix molecules, such as fibronectin and type I collagen. A variety of cytokines and growth factors are involved in the formation of the fibrotic membrane. Although gremlin has been reported to serve an important role in the regulation of epithelial-to-mesenchymal transition in PVR, the relationship between gremlin and the expression of profibrogenic factors in human RPE cells remains unclear. In the present study, gremlin promoted RPE cell proliferation and the expression of type I collagen and fibronectin. In addition, knocking down gremlin expression by siRNA significantly suppressed the transforming growth factor (TGF)-β1- and TGF-β2-induced expression of type I collagen and fibronectin in RPE cells. These findings suggest that gremlin may serve an important role in the development of PVR.
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spelling pubmed-70272312020-02-26 Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells Qin, Dong Jin, Xuemin Jiang, Yanrong Exp Ther Med Articles Proliferative vitreoretinopathy (PVR) is characterised by the contraction and growth of fibrotic membranes on the retina and within the vitreous body. Retinal pigment epithelial (RPE) cells, a major cellular component of the fibrotic membrane, is one of the cell types that have been previously reported to associate with PVR pathogenesis. During PVR, RPE cells undergo increased cell proliferation, migration and the secretion of extracellular matrix molecules, such as fibronectin and type I collagen. A variety of cytokines and growth factors are involved in the formation of the fibrotic membrane. Although gremlin has been reported to serve an important role in the regulation of epithelial-to-mesenchymal transition in PVR, the relationship between gremlin and the expression of profibrogenic factors in human RPE cells remains unclear. In the present study, gremlin promoted RPE cell proliferation and the expression of type I collagen and fibronectin. In addition, knocking down gremlin expression by siRNA significantly suppressed the transforming growth factor (TGF)-β1- and TGF-β2-induced expression of type I collagen and fibronectin in RPE cells. These findings suggest that gremlin may serve an important role in the development of PVR. D.A. Spandidos 2020-03 2020-01-21 /pmc/articles/PMC7027231/ /pubmed/32104303 http://dx.doi.org/10.3892/etm.2020.8463 Text en Copyright: © Qin et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Qin, Dong
Jin, Xuemin
Jiang, Yanrong
Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells
title Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells
title_full Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells
title_fullStr Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells
title_full_unstemmed Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells
title_short Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells
title_sort gremlin mediates the tgf-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027231/
https://www.ncbi.nlm.nih.gov/pubmed/32104303
http://dx.doi.org/10.3892/etm.2020.8463
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