Insights into the roles of sperm in animal cloning

Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somat...

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Detalles Bibliográficos
Autores principales: Qu, Pengxiang, Wang, Yongsheng, Zhang, Chengsheng, Liu, Enqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027237/
https://www.ncbi.nlm.nih.gov/pubmed/32070430
http://dx.doi.org/10.1186/s13287-020-01599-6
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author Qu, Pengxiang
Wang, Yongsheng
Zhang, Chengsheng
Liu, Enqi
author_facet Qu, Pengxiang
Wang, Yongsheng
Zhang, Chengsheng
Liu, Enqi
author_sort Qu, Pengxiang
collection PubMed
description Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somatic donor cells lack some important components of sperm, such as sperm small noncoding RNA (sncRNA) and proteins. Loss of these factors is considered an important reason for the abnormal development of SCNT embryo. This study focused on recent advances of SCNT and the roles of sperm in development. Sperm-derived factors play an important role in nucleus reprogramming and cytoskeleton remodeling during SCNT embryo development. Hence, considering the role of sperm may provide a new strategy for improving cloning efficiency.
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spelling pubmed-70272372020-02-24 Insights into the roles of sperm in animal cloning Qu, Pengxiang Wang, Yongsheng Zhang, Chengsheng Liu, Enqi Stem Cell Res Ther Review Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somatic donor cells lack some important components of sperm, such as sperm small noncoding RNA (sncRNA) and proteins. Loss of these factors is considered an important reason for the abnormal development of SCNT embryo. This study focused on recent advances of SCNT and the roles of sperm in development. Sperm-derived factors play an important role in nucleus reprogramming and cytoskeleton remodeling during SCNT embryo development. Hence, considering the role of sperm may provide a new strategy for improving cloning efficiency. BioMed Central 2020-02-18 /pmc/articles/PMC7027237/ /pubmed/32070430 http://dx.doi.org/10.1186/s13287-020-01599-6 Text en © The Author(s) 2020 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Review
Qu, Pengxiang
Wang, Yongsheng
Zhang, Chengsheng
Liu, Enqi
Insights into the roles of sperm in animal cloning
title Insights into the roles of sperm in animal cloning
title_full Insights into the roles of sperm in animal cloning
title_fullStr Insights into the roles of sperm in animal cloning
title_full_unstemmed Insights into the roles of sperm in animal cloning
title_short Insights into the roles of sperm in animal cloning
title_sort insights into the roles of sperm in animal cloning
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027237/
https://www.ncbi.nlm.nih.gov/pubmed/32070430
http://dx.doi.org/10.1186/s13287-020-01599-6
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AT wangyongsheng insightsintotherolesofsperminanimalcloning
AT zhangchengsheng insightsintotherolesofsperminanimalcloning
AT liuenqi insightsintotherolesofsperminanimalcloning

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