Effects of hypoxic-ischemic pre-treatment on microvesicles derived from endothelial progenitor cells

Endothelial progenitor cells (EPCs) have protective roles in ischemic injury due to their ability to improve endothelial function and modulate angiogenesis. Microvesicles (MVs) are small membrane particles released by various cell types, including EPCs, which affect various target cells by transferring carried genetic information, including microRNAs (miRNAs/miRs). Depending on the stimuli and cell types, MVs exert different functions. In the present study, oxygen-glucose deprivation (OGD) was used to mimic ischemic-hypoxic (HI) insult, where the effects of HI insult on EPC-derived MVs (EPC-MVs) were subsequently investigated. OGD induced Ca(2+) influx in EPCs and increased the release of EPC-MVs compared with normoxic conditions. In addition, MVs prepared from EPCs cultured under normoxic conditions or OGD conditions (OGD-EMVs) had the ability to stimulate the proliferation of EPCs. Furthermore, OGD-EMVs induced stronger effects on proliferation, which may be associated with the upregulation of miR-210 in EPC-MVs. In conclusion, the present results indicated that HI insult promoted the release of MVs from EPCs and upregulated miR-210 in MVs, leading to positive modulation of the proliferation of EPCs cultured under normoxic conditions.