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Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti

Transinfections of the maternally transmitted endosymbiont Wolbachia pipientis can reduce RNA virus replication and prevent transmission by Aedes aegypti, and also have the capacity to invade wild‐type populations, potentially reaching and maintaining high infection frequencies. Levels of virus tran...

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Autores principales: Chouin‐Carneiro, T., Ant, T. H., Herd, C., Louis, F., Failloux, A. B., Sinkins, S. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027442/
https://www.ncbi.nlm.nih.gov/pubmed/31120156
http://dx.doi.org/10.1111/mve.12384
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author Chouin‐Carneiro, T.
Ant, T. H.
Herd, C.
Louis, F.
Failloux, A. B.
Sinkins, S. P.
author_facet Chouin‐Carneiro, T.
Ant, T. H.
Herd, C.
Louis, F.
Failloux, A. B.
Sinkins, S. P.
author_sort Chouin‐Carneiro, T.
collection PubMed
description Transinfections of the maternally transmitted endosymbiont Wolbachia pipientis can reduce RNA virus replication and prevent transmission by Aedes aegypti, and also have the capacity to invade wild‐type populations, potentially reaching and maintaining high infection frequencies. Levels of virus transmission blocking are positively correlated with Wolbachia intracellular density. Despite reaching high densities in Ae. aegypti, transinfections of wAlbA, a strain native to Aedes albopictus, showed no blocking of Semliki Forest Virus in previous intrathoracic injection challenges. To further characterize wAlbA blocking in Ae. aegypti, adult females were intrathoracically challenged with Zika (ZIKV) and dengue viruses, and then fed a ZIKV‐containing bloodmeal. No blocking was observed with either virus when challenged by intrathoracic injection. However, when ZIKV was delivered orally, wAlbA‐infected females showed a significant reduction in viral replication and dissemination compared with uninfected controls, as well as a complete absence of virus in saliva. Although other Wolbachia strains have been shown to cause more robust viral blocking in Ae. aegypti, these findings demonstrate that, in principle, wAlbA could be used to reduce virus transmission in this species. Moreover, the results highlight the potential for underestimation of the strength of virus‐blocking when based on intrathoracic injection compared with more natural oral challenges.
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spelling pubmed-70274422020-02-24 Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti Chouin‐Carneiro, T. Ant, T. H. Herd, C. Louis, F. Failloux, A. B. Sinkins, S. P. Med Vet Entomol Short Communications Transinfections of the maternally transmitted endosymbiont Wolbachia pipientis can reduce RNA virus replication and prevent transmission by Aedes aegypti, and also have the capacity to invade wild‐type populations, potentially reaching and maintaining high infection frequencies. Levels of virus transmission blocking are positively correlated with Wolbachia intracellular density. Despite reaching high densities in Ae. aegypti, transinfections of wAlbA, a strain native to Aedes albopictus, showed no blocking of Semliki Forest Virus in previous intrathoracic injection challenges. To further characterize wAlbA blocking in Ae. aegypti, adult females were intrathoracically challenged with Zika (ZIKV) and dengue viruses, and then fed a ZIKV‐containing bloodmeal. No blocking was observed with either virus when challenged by intrathoracic injection. However, when ZIKV was delivered orally, wAlbA‐infected females showed a significant reduction in viral replication and dissemination compared with uninfected controls, as well as a complete absence of virus in saliva. Although other Wolbachia strains have been shown to cause more robust viral blocking in Ae. aegypti, these findings demonstrate that, in principle, wAlbA could be used to reduce virus transmission in this species. Moreover, the results highlight the potential for underestimation of the strength of virus‐blocking when based on intrathoracic injection compared with more natural oral challenges. Blackwell Publishing Ltd 2019-05-23 2020-03 /pmc/articles/PMC7027442/ /pubmed/31120156 http://dx.doi.org/10.1111/mve.12384 Text en © 2019 The Authors. Medical and Veterinary Entomology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communications
Chouin‐Carneiro, T.
Ant, T. H.
Herd, C.
Louis, F.
Failloux, A. B.
Sinkins, S. P.
Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti
title Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti
title_full Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti
title_fullStr Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti
title_full_unstemmed Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti
title_short Wolbachia strain wAlbA blocks Zika virus transmission in Aedes aegypti
title_sort wolbachia strain walba blocks zika virus transmission in aedes aegypti
topic Short Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7027442/
https://www.ncbi.nlm.nih.gov/pubmed/31120156
http://dx.doi.org/10.1111/mve.12384
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