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A safety type of genetically engineered bacterium that degrades chemical pesticides

Chemical pesticides are used widely and their residues are found in the environment. Pesticide pollution has become a global problem. To find an economical, effective and safety way to degrade residues of pesticides in environment, we constructed a genetically engineered bacterium (GEB) having the a...

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Autores principales: Li, Qin, Li, Jing, Kang, Ke-Lai, Wu, Yi-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028883/
https://www.ncbi.nlm.nih.gov/pubmed/32072335
http://dx.doi.org/10.1186/s13568-020-00967-y
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author Li, Qin
Li, Jing
Kang, Ke-Lai
Wu, Yi-Jun
author_facet Li, Qin
Li, Jing
Kang, Ke-Lai
Wu, Yi-Jun
author_sort Li, Qin
collection PubMed
description Chemical pesticides are used widely and their residues are found in the environment. Pesticide pollution has become a global problem. To find an economical, effective and safety way to degrade residues of pesticides in environment, we constructed a genetically engineered bacterium (GEB) having the ability to degrade pesticides, emit green fluorescence and has a containment system by using a dual plasmid expression system. One plasmid contains the genes of enhanced green fluorescent protein (EGFP) and carboxylesterase B1 (CarE B1), which were cloned downstream of lambda P(L) promoter and expressed constitutively. The gene of CarE B1 encodes an insect-detoxifying enzyme possessing the degradability to organochloride pesticides, organophosphorus pesticides, carbamates, and pyrethoid insecticides. The other is the conditional suicide plasmid for containment system, in which the lethal gene used was the nuclease gene of Serratia marcescens without the leader-coding sequence and was placed downstream of T7 promoter. The GEB has wide prospects of application on cleanup of pesticide residues with its degradability to several pesticides and containment system.
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spelling pubmed-70288832020-03-05 A safety type of genetically engineered bacterium that degrades chemical pesticides Li, Qin Li, Jing Kang, Ke-Lai Wu, Yi-Jun AMB Express Original Article Chemical pesticides are used widely and their residues are found in the environment. Pesticide pollution has become a global problem. To find an economical, effective and safety way to degrade residues of pesticides in environment, we constructed a genetically engineered bacterium (GEB) having the ability to degrade pesticides, emit green fluorescence and has a containment system by using a dual plasmid expression system. One plasmid contains the genes of enhanced green fluorescent protein (EGFP) and carboxylesterase B1 (CarE B1), which were cloned downstream of lambda P(L) promoter and expressed constitutively. The gene of CarE B1 encodes an insect-detoxifying enzyme possessing the degradability to organochloride pesticides, organophosphorus pesticides, carbamates, and pyrethoid insecticides. The other is the conditional suicide plasmid for containment system, in which the lethal gene used was the nuclease gene of Serratia marcescens without the leader-coding sequence and was placed downstream of T7 promoter. The GEB has wide prospects of application on cleanup of pesticide residues with its degradability to several pesticides and containment system. Springer Berlin Heidelberg 2020-02-18 /pmc/articles/PMC7028883/ /pubmed/32072335 http://dx.doi.org/10.1186/s13568-020-00967-y Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Original Article
Li, Qin
Li, Jing
Kang, Ke-Lai
Wu, Yi-Jun
A safety type of genetically engineered bacterium that degrades chemical pesticides
title A safety type of genetically engineered bacterium that degrades chemical pesticides
title_full A safety type of genetically engineered bacterium that degrades chemical pesticides
title_fullStr A safety type of genetically engineered bacterium that degrades chemical pesticides
title_full_unstemmed A safety type of genetically engineered bacterium that degrades chemical pesticides
title_short A safety type of genetically engineered bacterium that degrades chemical pesticides
title_sort safety type of genetically engineered bacterium that degrades chemical pesticides
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7028883/
https://www.ncbi.nlm.nih.gov/pubmed/32072335
http://dx.doi.org/10.1186/s13568-020-00967-y
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