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Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands
Staphylococcus epidermidis is a leading cause of nosocomial infections in patients with a compromised immune system and/or an implanted medical device. Seventy to 90% of S. epidermidis clinical isolates are methicillin resistant and carry the mecA gene, present in a mobile genetic element (MGE) call...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029136/ https://www.ncbi.nlm.nih.gov/pubmed/32071265 http://dx.doi.org/10.1128/mBio.02911-19 |
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author | Arora, Srishtee Li, Xiqi Hillhouse, Andrew Konganti, Kranti Little, Sara V. Lawhon, Sara D. Threadgill, David Shelburne, Samuel Hook, Magnus |
author_facet | Arora, Srishtee Li, Xiqi Hillhouse, Andrew Konganti, Kranti Little, Sara V. Lawhon, Sara D. Threadgill, David Shelburne, Samuel Hook, Magnus |
author_sort | Arora, Srishtee |
collection | PubMed |
description | Staphylococcus epidermidis is a leading cause of nosocomial infections in patients with a compromised immune system and/or an implanted medical device. Seventy to 90% of S. epidermidis clinical isolates are methicillin resistant and carry the mecA gene, present in a mobile genetic element (MGE) called the staphylococcal cassette chromosome mec (SCCmec) element. Along with the presence of antibiotic and heavy metal resistance genes, MGEs can also contain genes encoding secreted or cell wall-anchored virulence factors. In our earlier studies of S. epidermidis clinical isolates, we discovered S. epidermidis surface protein J (SesJ), a prototype of a recently discovered subfamily of the microbial surface component recognizing adhesive matrix molecule (MSCRAMM) group. MSCRAMMs are major virulence factors of pathogenic Gram-positive bacteria. Here, we report that the sesJ gene is always accompanied by two glycosyltransferase genes, gtfA and gtfB, and is present in two MGEs, called the arginine catabolic mobile element (ACME) and the staphylococcal cassette chromosome (SCC) element. The presence of the sesJ gene was associated with the left-hand direct repeat DR_B or DR_E. When inserted via DR_E, the sesJ gene was encoded in the SCC element. When inserted via DR_B, the sesJ gene was accompanied by the genes for the type 1 restriction modification system and was encoded in the ACME. Additionally, the SCC element and ACME carry different isoforms of the SesJ protein. To date, the genes encoding MSCRAMMs have been seen to be located in the bacterial core genome. Here, we report the presence of an MSCRAMM in an MGE in S. epidermidis clinical isolates. |
format | Online Article Text |
id | pubmed-7029136 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-70291362020-02-26 Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands Arora, Srishtee Li, Xiqi Hillhouse, Andrew Konganti, Kranti Little, Sara V. Lawhon, Sara D. Threadgill, David Shelburne, Samuel Hook, Magnus mBio Research Article Staphylococcus epidermidis is a leading cause of nosocomial infections in patients with a compromised immune system and/or an implanted medical device. Seventy to 90% of S. epidermidis clinical isolates are methicillin resistant and carry the mecA gene, present in a mobile genetic element (MGE) called the staphylococcal cassette chromosome mec (SCCmec) element. Along with the presence of antibiotic and heavy metal resistance genes, MGEs can also contain genes encoding secreted or cell wall-anchored virulence factors. In our earlier studies of S. epidermidis clinical isolates, we discovered S. epidermidis surface protein J (SesJ), a prototype of a recently discovered subfamily of the microbial surface component recognizing adhesive matrix molecule (MSCRAMM) group. MSCRAMMs are major virulence factors of pathogenic Gram-positive bacteria. Here, we report that the sesJ gene is always accompanied by two glycosyltransferase genes, gtfA and gtfB, and is present in two MGEs, called the arginine catabolic mobile element (ACME) and the staphylococcal cassette chromosome (SCC) element. The presence of the sesJ gene was associated with the left-hand direct repeat DR_B or DR_E. When inserted via DR_E, the sesJ gene was encoded in the SCC element. When inserted via DR_B, the sesJ gene was accompanied by the genes for the type 1 restriction modification system and was encoded in the ACME. Additionally, the SCC element and ACME carry different isoforms of the SesJ protein. To date, the genes encoding MSCRAMMs have been seen to be located in the bacterial core genome. Here, we report the presence of an MSCRAMM in an MGE in S. epidermidis clinical isolates. American Society for Microbiology 2020-02-18 /pmc/articles/PMC7029136/ /pubmed/32071265 http://dx.doi.org/10.1128/mBio.02911-19 Text en Copyright © 2020 Arora et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Arora, Srishtee Li, Xiqi Hillhouse, Andrew Konganti, Kranti Little, Sara V. Lawhon, Sara D. Threadgill, David Shelburne, Samuel Hook, Magnus Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands |
title | Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands |
title_full | Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands |
title_fullStr | Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands |
title_full_unstemmed | Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands |
title_short | Staphylococcus epidermidis MSCRAMM SesJ Is Encoded in Composite Islands |
title_sort | staphylococcus epidermidis mscramm sesj is encoded in composite islands |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029136/ https://www.ncbi.nlm.nih.gov/pubmed/32071265 http://dx.doi.org/10.1128/mBio.02911-19 |
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