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Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer

BACKGROUND: Accumulating evidence has demonstrated that microRNA-200s (miR-200a, miR-200b and miR-200c) could serve as promising molecular biomarkers for cancer prognosis. Nevertheless, the associations between miR-200s expression and colorectal cancer (CRC) prognosis remain controversial. METHODS:...

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Autores principales: Peng, Qiliang, Cheng, Ming, Li, Ting, Chen, Xiangying, Shen, Yi, Zhu, Yaqun, Xu, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029504/
https://www.ncbi.nlm.nih.gov/pubmed/32099529
http://dx.doi.org/10.1186/s12935-020-1142-1
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author Peng, Qiliang
Cheng, Ming
Li, Ting
Chen, Xiangying
Shen, Yi
Zhu, Yaqun
Xu, Bo
author_facet Peng, Qiliang
Cheng, Ming
Li, Ting
Chen, Xiangying
Shen, Yi
Zhu, Yaqun
Xu, Bo
author_sort Peng, Qiliang
collection PubMed
description BACKGROUND: Accumulating evidence has demonstrated that microRNA-200s (miR-200a, miR-200b and miR-200c) could serve as promising molecular biomarkers for cancer prognosis. Nevertheless, the associations between miR-200s expression and colorectal cancer (CRC) prognosis remain controversial. METHODS: We applied two mainstream approaches combining meta-analysis and bioinformatics analysis to answer whether miR-200s were associated with the prognosis of CRC patients and why miR-200s could be used as prognostic biomarkers for CRC. RESULTS: Consequently, low expression of miR-200s was associated with unfavorable overall survival (OS) in CRC patients (HR: 1.09; 95% CI 1.01–1.17; P = 0.025). According to the subgroup analysis, the prognostic role of miR-200s was more significant for tissue samples, large samples, American patients and miR-200a subgroups. Then the target genes of miR-200s were predicted and applied for functional enrichment analyses. The results showed that the target genes of miR-200s were mainly enriched into some vital ontology subjects such as regulation ability, key cell structures and binding function. Moreover, a series of important signaling pathways were identified, which were significantly linked with the initiation and progression of CRC. Additionally, a protein‑protein interaction (PPI) network of miR-200s targets was constructed to screen hub genes and modules. The identified hub genes and modules were validated to be highly involved in the occurrence and development of CRC. CONCLUSIONS: Current evidences revealed that miR-200s could be promising biomarkers for CRC prognosis. However, the findings still need to be validated with more larger-scale prospective studies and biological experiments before miR-200s could be applied into clinical application.
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spelling pubmed-70295042020-02-25 Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer Peng, Qiliang Cheng, Ming Li, Ting Chen, Xiangying Shen, Yi Zhu, Yaqun Xu, Bo Cancer Cell Int Primary Research BACKGROUND: Accumulating evidence has demonstrated that microRNA-200s (miR-200a, miR-200b and miR-200c) could serve as promising molecular biomarkers for cancer prognosis. Nevertheless, the associations between miR-200s expression and colorectal cancer (CRC) prognosis remain controversial. METHODS: We applied two mainstream approaches combining meta-analysis and bioinformatics analysis to answer whether miR-200s were associated with the prognosis of CRC patients and why miR-200s could be used as prognostic biomarkers for CRC. RESULTS: Consequently, low expression of miR-200s was associated with unfavorable overall survival (OS) in CRC patients (HR: 1.09; 95% CI 1.01–1.17; P = 0.025). According to the subgroup analysis, the prognostic role of miR-200s was more significant for tissue samples, large samples, American patients and miR-200a subgroups. Then the target genes of miR-200s were predicted and applied for functional enrichment analyses. The results showed that the target genes of miR-200s were mainly enriched into some vital ontology subjects such as regulation ability, key cell structures and binding function. Moreover, a series of important signaling pathways were identified, which were significantly linked with the initiation and progression of CRC. Additionally, a protein‑protein interaction (PPI) network of miR-200s targets was constructed to screen hub genes and modules. The identified hub genes and modules were validated to be highly involved in the occurrence and development of CRC. CONCLUSIONS: Current evidences revealed that miR-200s could be promising biomarkers for CRC prognosis. However, the findings still need to be validated with more larger-scale prospective studies and biological experiments before miR-200s could be applied into clinical application. BioMed Central 2020-02-18 /pmc/articles/PMC7029504/ /pubmed/32099529 http://dx.doi.org/10.1186/s12935-020-1142-1 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Peng, Qiliang
Cheng, Ming
Li, Ting
Chen, Xiangying
Shen, Yi
Zhu, Yaqun
Xu, Bo
Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer
title Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer
title_full Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer
title_fullStr Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer
title_full_unstemmed Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer
title_short Integrated characterization and validation of the prognostic significance of microRNA-200s in colorectal cancer
title_sort integrated characterization and validation of the prognostic significance of microrna-200s in colorectal cancer
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029504/
https://www.ncbi.nlm.nih.gov/pubmed/32099529
http://dx.doi.org/10.1186/s12935-020-1142-1
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