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Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury

BACKGROUND: Intra-abdominal hypertension (IAH) is associated with high morbidity and mortality. IAH leads to intra-abdominal tissue damage and causes dysfunction in distal organs such as the brain. The effect of a combined injury due to IAH and traumatic brain injury (TBI) on the integrity of the bl...

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Autores principales: Chen, Peng, Tang, Hao, Zhang, Qingtao, Xu, Lei, Zhou, Wei, Hu, Xi, Deng, Yongbing, Zhang, Lianyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029819/
https://www.ncbi.nlm.nih.gov/pubmed/32036381
http://dx.doi.org/10.12659/MSM.922009
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author Chen, Peng
Tang, Hao
Zhang, Qingtao
Xu, Lei
Zhou, Wei
Hu, Xi
Deng, Yongbing
Zhang, Lianyang
author_facet Chen, Peng
Tang, Hao
Zhang, Qingtao
Xu, Lei
Zhou, Wei
Hu, Xi
Deng, Yongbing
Zhang, Lianyang
author_sort Chen, Peng
collection PubMed
description BACKGROUND: Intra-abdominal hypertension (IAH) is associated with high morbidity and mortality. IAH leads to intra-abdominal tissue damage and causes dysfunction in distal organs such as the brain. The effect of a combined injury due to IAH and traumatic brain injury (TBI) on the integrity of the blood–brain barrier (BBB) has not been investigated. MATERIAL/METHODS: Intracranial pressure (ICP) monitoring, brain water content, EB permeability detection, immunofluorescence staining, real-time PCR, and Western blot analysis were used to examine the effects of IAH and TBI on the BBB in rats, and to characterize the protective effects of basic fibroblast growth factor (bFGF) on combined injury-induced BBB damage. RESULTS: Combined injury from IAH and TBI to the BBB resulted in brain edema and increased intracranial pressure. The effects of bFGF on alleviating the rat BBB injuries were determined, indicating that bFGF regulated the expression levels of the tight junction (TJ), adhesion junction (AJ), matrix metalloproteinase (MMP), and IL-1β, as well as reduced BBB permeability, brain edema, and intracranial pressure. Moreover, the FGFR1 antagonist PD 173074 and the ERK antagonist PD 98059 decreased the protective effects of bFGF. CONCLUSIONS: bFGF effectively protected the BBB from damage caused by combined injury from IAH and TBI, and binding of FGFR1 and activation of the ERK signaling pathway was involved in these effects.
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spelling pubmed-70298192020-03-05 Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury Chen, Peng Tang, Hao Zhang, Qingtao Xu, Lei Zhou, Wei Hu, Xi Deng, Yongbing Zhang, Lianyang Med Sci Monit Animal Study BACKGROUND: Intra-abdominal hypertension (IAH) is associated with high morbidity and mortality. IAH leads to intra-abdominal tissue damage and causes dysfunction in distal organs such as the brain. The effect of a combined injury due to IAH and traumatic brain injury (TBI) on the integrity of the blood–brain barrier (BBB) has not been investigated. MATERIAL/METHODS: Intracranial pressure (ICP) monitoring, brain water content, EB permeability detection, immunofluorescence staining, real-time PCR, and Western blot analysis were used to examine the effects of IAH and TBI on the BBB in rats, and to characterize the protective effects of basic fibroblast growth factor (bFGF) on combined injury-induced BBB damage. RESULTS: Combined injury from IAH and TBI to the BBB resulted in brain edema and increased intracranial pressure. The effects of bFGF on alleviating the rat BBB injuries were determined, indicating that bFGF regulated the expression levels of the tight junction (TJ), adhesion junction (AJ), matrix metalloproteinase (MMP), and IL-1β, as well as reduced BBB permeability, brain edema, and intracranial pressure. Moreover, the FGFR1 antagonist PD 173074 and the ERK antagonist PD 98059 decreased the protective effects of bFGF. CONCLUSIONS: bFGF effectively protected the BBB from damage caused by combined injury from IAH and TBI, and binding of FGFR1 and activation of the ERK signaling pathway was involved in these effects. International Scientific Literature, Inc. 2020-02-09 /pmc/articles/PMC7029819/ /pubmed/32036381 http://dx.doi.org/10.12659/MSM.922009 Text en © Med Sci Monit, 2020 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Animal Study
Chen, Peng
Tang, Hao
Zhang, Qingtao
Xu, Lei
Zhou, Wei
Hu, Xi
Deng, Yongbing
Zhang, Lianyang
Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury
title Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury
title_full Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury
title_fullStr Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury
title_full_unstemmed Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury
title_short Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury
title_sort basic fibroblast growth factor (bfgf) protects the blood–brain barrier by binding of fgfr1 and activating the erk signaling pathway after intra-abdominal hypertension and traumatic brain injury
topic Animal Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029819/
https://www.ncbi.nlm.nih.gov/pubmed/32036381
http://dx.doi.org/10.12659/MSM.922009
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