Cargando…
Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury
BACKGROUND: Intra-abdominal hypertension (IAH) is associated with high morbidity and mortality. IAH leads to intra-abdominal tissue damage and causes dysfunction in distal organs such as the brain. The effect of a combined injury due to IAH and traumatic brain injury (TBI) on the integrity of the bl...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Scientific Literature, Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029819/ https://www.ncbi.nlm.nih.gov/pubmed/32036381 http://dx.doi.org/10.12659/MSM.922009 |
_version_ | 1783499234901753856 |
---|---|
author | Chen, Peng Tang, Hao Zhang, Qingtao Xu, Lei Zhou, Wei Hu, Xi Deng, Yongbing Zhang, Lianyang |
author_facet | Chen, Peng Tang, Hao Zhang, Qingtao Xu, Lei Zhou, Wei Hu, Xi Deng, Yongbing Zhang, Lianyang |
author_sort | Chen, Peng |
collection | PubMed |
description | BACKGROUND: Intra-abdominal hypertension (IAH) is associated with high morbidity and mortality. IAH leads to intra-abdominal tissue damage and causes dysfunction in distal organs such as the brain. The effect of a combined injury due to IAH and traumatic brain injury (TBI) on the integrity of the blood–brain barrier (BBB) has not been investigated. MATERIAL/METHODS: Intracranial pressure (ICP) monitoring, brain water content, EB permeability detection, immunofluorescence staining, real-time PCR, and Western blot analysis were used to examine the effects of IAH and TBI on the BBB in rats, and to characterize the protective effects of basic fibroblast growth factor (bFGF) on combined injury-induced BBB damage. RESULTS: Combined injury from IAH and TBI to the BBB resulted in brain edema and increased intracranial pressure. The effects of bFGF on alleviating the rat BBB injuries were determined, indicating that bFGF regulated the expression levels of the tight junction (TJ), adhesion junction (AJ), matrix metalloproteinase (MMP), and IL-1β, as well as reduced BBB permeability, brain edema, and intracranial pressure. Moreover, the FGFR1 antagonist PD 173074 and the ERK antagonist PD 98059 decreased the protective effects of bFGF. CONCLUSIONS: bFGF effectively protected the BBB from damage caused by combined injury from IAH and TBI, and binding of FGFR1 and activation of the ERK signaling pathway was involved in these effects. |
format | Online Article Text |
id | pubmed-7029819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | International Scientific Literature, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70298192020-03-05 Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury Chen, Peng Tang, Hao Zhang, Qingtao Xu, Lei Zhou, Wei Hu, Xi Deng, Yongbing Zhang, Lianyang Med Sci Monit Animal Study BACKGROUND: Intra-abdominal hypertension (IAH) is associated with high morbidity and mortality. IAH leads to intra-abdominal tissue damage and causes dysfunction in distal organs such as the brain. The effect of a combined injury due to IAH and traumatic brain injury (TBI) on the integrity of the blood–brain barrier (BBB) has not been investigated. MATERIAL/METHODS: Intracranial pressure (ICP) monitoring, brain water content, EB permeability detection, immunofluorescence staining, real-time PCR, and Western blot analysis were used to examine the effects of IAH and TBI on the BBB in rats, and to characterize the protective effects of basic fibroblast growth factor (bFGF) on combined injury-induced BBB damage. RESULTS: Combined injury from IAH and TBI to the BBB resulted in brain edema and increased intracranial pressure. The effects of bFGF on alleviating the rat BBB injuries were determined, indicating that bFGF regulated the expression levels of the tight junction (TJ), adhesion junction (AJ), matrix metalloproteinase (MMP), and IL-1β, as well as reduced BBB permeability, brain edema, and intracranial pressure. Moreover, the FGFR1 antagonist PD 173074 and the ERK antagonist PD 98059 decreased the protective effects of bFGF. CONCLUSIONS: bFGF effectively protected the BBB from damage caused by combined injury from IAH and TBI, and binding of FGFR1 and activation of the ERK signaling pathway was involved in these effects. International Scientific Literature, Inc. 2020-02-09 /pmc/articles/PMC7029819/ /pubmed/32036381 http://dx.doi.org/10.12659/MSM.922009 Text en © Med Sci Monit, 2020 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) ) |
spellingShingle | Animal Study Chen, Peng Tang, Hao Zhang, Qingtao Xu, Lei Zhou, Wei Hu, Xi Deng, Yongbing Zhang, Lianyang Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury |
title | Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury |
title_full | Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury |
title_fullStr | Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury |
title_full_unstemmed | Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury |
title_short | Basic Fibroblast Growth Factor (bFGF) Protects the Blood–Brain Barrier by Binding of FGFR1 and Activating the ERK Signaling Pathway After Intra-Abdominal Hypertension and Traumatic Brain Injury |
title_sort | basic fibroblast growth factor (bfgf) protects the blood–brain barrier by binding of fgfr1 and activating the erk signaling pathway after intra-abdominal hypertension and traumatic brain injury |
topic | Animal Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029819/ https://www.ncbi.nlm.nih.gov/pubmed/32036381 http://dx.doi.org/10.12659/MSM.922009 |
work_keys_str_mv | AT chenpeng basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury AT tanghao basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury AT zhangqingtao basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury AT xulei basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury AT zhouwei basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury AT huxi basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury AT dengyongbing basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury AT zhanglianyang basicfibroblastgrowthfactorbfgfprotectsthebloodbrainbarrierbybindingoffgfr1andactivatingtheerksignalingpathwayafterintraabdominalhypertensionandtraumaticbraininjury |