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Populus euphratica WRKY1 binds the promoter of H(+)-ATPase gene to enhance gene expression and salt tolerance

Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translation...

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Detalles Bibliográficos
Autores principales: Yao, Jun, Shen, Zedan, Zhang, Yanli, Wu, Xia, Wang, Jianhui, Sa, Gang, Zhang, Yuhong, Zhang, Huilong, Deng, Chen, Liu, Jian, Hou, Siyuan, Zhang, Ying, Zhang, Yinan, Zhao, Nan, Deng, Shurong, Lin, Shanzhi, Zhao, Rui, Chen, Shaoliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031066/
https://www.ncbi.nlm.nih.gov/pubmed/31680166
http://dx.doi.org/10.1093/jxb/erz493
Descripción
Sumario:Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica. The promoter–reporter construct PeHA1-pro::GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica. PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis-acting W-box in the regulatory region, and improved H(+) pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1. Thus, we conclude that enhanced H(+) pumping activity enabled salt-stressed plants to retain Na(+) homeostasis.