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Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens
Caligus rogercresseyi is a copepod ectoparasite with a high prevalence in salmon farms in Chile, causing severe welfare and economic concerns to the sector. Information on the parasite’s underpinning mechanisms to support its life strategy is recently being investigated. Due to the critical role of...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031262/ https://www.ncbi.nlm.nih.gov/pubmed/32076035 http://dx.doi.org/10.1038/s41598-020-59747-0 |
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author | Gonçalves, Ana Teresa Collipal-Matamal, Rayen Valenzuela-Muñoz, Valentina Nuñez-Acuña, Gustavo Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian |
author_facet | Gonçalves, Ana Teresa Collipal-Matamal, Rayen Valenzuela-Muñoz, Valentina Nuñez-Acuña, Gustavo Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian |
author_sort | Gonçalves, Ana Teresa |
collection | PubMed |
description | Caligus rogercresseyi is a copepod ectoparasite with a high prevalence in salmon farms in Chile, causing severe welfare and economic concerns to the sector. Information on the parasite’s underpinning mechanisms to support its life strategy is recently being investigated. Due to the critical role of microbiota, this study aimed to characterize the microbiota community associated with C. rogercresseyi from different regions with salmon aquaculture in Chile. Using third-generation sequencing with Nanopore technology (MinION) the full 16S rRNA gene from sea lice obtained from 8 areas distributed over the three main aquaculture regions were sequenced. Microbiota of the parasite is mainly comprised of members of phyla Proteobacteria and Bacteroidetes, and a core microbiota community with 147 taxonomical features was identified, and it was present in sea lice from the three regions. This community accounted for 19% of total identified taxa but more than 70% of the total taxonomical abundance, indicating a strong presence in the parasite. Several taxa with bioactive compound secretory capacity were identified, such as members of genus Pseudoalteromonas and Dokdonia, suggesting a possible role of the lice microbiota during the host infestation processes. Furthermore, the microbiota community was differentially associated with the salmon production, where several potential pathogens such as Vibrio, Tenacibaculum, and Aeromonas in Los Lagos, Aysén, and Magallanes region were identified. Notably, the Chilean salmon industry was initially established in the Los Lagos region but it’s currently moving to the south, where different oceanographic conditions coexist with lice populations. The results originated by this study will serve as foundation to investigate putative role of sea lice as vectors for fish pathogens and also as reservoirs for antibiotic-resistant genes. |
format | Online Article Text |
id | pubmed-7031262 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70312622020-02-27 Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens Gonçalves, Ana Teresa Collipal-Matamal, Rayen Valenzuela-Muñoz, Valentina Nuñez-Acuña, Gustavo Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian Sci Rep Article Caligus rogercresseyi is a copepod ectoparasite with a high prevalence in salmon farms in Chile, causing severe welfare and economic concerns to the sector. Information on the parasite’s underpinning mechanisms to support its life strategy is recently being investigated. Due to the critical role of microbiota, this study aimed to characterize the microbiota community associated with C. rogercresseyi from different regions with salmon aquaculture in Chile. Using third-generation sequencing with Nanopore technology (MinION) the full 16S rRNA gene from sea lice obtained from 8 areas distributed over the three main aquaculture regions were sequenced. Microbiota of the parasite is mainly comprised of members of phyla Proteobacteria and Bacteroidetes, and a core microbiota community with 147 taxonomical features was identified, and it was present in sea lice from the three regions. This community accounted for 19% of total identified taxa but more than 70% of the total taxonomical abundance, indicating a strong presence in the parasite. Several taxa with bioactive compound secretory capacity were identified, such as members of genus Pseudoalteromonas and Dokdonia, suggesting a possible role of the lice microbiota during the host infestation processes. Furthermore, the microbiota community was differentially associated with the salmon production, where several potential pathogens such as Vibrio, Tenacibaculum, and Aeromonas in Los Lagos, Aysén, and Magallanes region were identified. Notably, the Chilean salmon industry was initially established in the Los Lagos region but it’s currently moving to the south, where different oceanographic conditions coexist with lice populations. The results originated by this study will serve as foundation to investigate putative role of sea lice as vectors for fish pathogens and also as reservoirs for antibiotic-resistant genes. Nature Publishing Group UK 2020-02-19 /pmc/articles/PMC7031262/ /pubmed/32076035 http://dx.doi.org/10.1038/s41598-020-59747-0 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Gonçalves, Ana Teresa Collipal-Matamal, Rayen Valenzuela-Muñoz, Valentina Nuñez-Acuña, Gustavo Valenzuela-Miranda, Diego Gallardo-Escárate, Cristian Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens |
title | Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens |
title_full | Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens |
title_fullStr | Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens |
title_full_unstemmed | Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens |
title_short | Nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens |
title_sort | nanopore sequencing of microbial communities reveals the potential role of sea lice as a reservoir for fish pathogens |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031262/ https://www.ncbi.nlm.nih.gov/pubmed/32076035 http://dx.doi.org/10.1038/s41598-020-59747-0 |
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