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DNA extraction of microbial DNA directly from infected tissue: an optimized protocol for use in nanopore sequencing
Identification of bacteria causing tissue infections can be comprehensive and, in the cases of non- or slow-growing bacteria, near impossible with conventional methods. Performing shotgun metagenomic sequencing on bacterial DNA extracted directly from the infected tissue may improve time to diagnosi...
Autores principales: | Helmersen, Karin, Aamot, Hege Vangstein |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031281/ https://www.ncbi.nlm.nih.gov/pubmed/32076089 http://dx.doi.org/10.1038/s41598-020-59957-6 |
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