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Establishment of tetracycline-regulated bimolecular fluorescence complementation assay to detect protein-protein interactions in Candida albicans

To visualize protein-protein interactions in Candida albicans with the bimolecular fluorescence complementation (BiFC) approach, we created a Tet-on system with the plasmids pWTN1 and pWTN2. Both plasmids bear a hygromycin B-resistant marker (CaHygB) that is compatible with the original Tet-on plasm...

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Detalles Bibliográficos
Autores principales: Lai, Wei-Chung, Sun, H. Sunny, Shieh, Jia-Ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031294/
https://www.ncbi.nlm.nih.gov/pubmed/32076074
http://dx.doi.org/10.1038/s41598-020-59891-7

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