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Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity
Antigenic variation, the capacity to produce a range of variable antigens, is a well-described strategy of Plasmodium and other parasites to evade host immunity. Here, we show that gene amplification is an additional evasion mechanism used by Plasmodium vivax to escape humoral immunity targeting PvD...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031336/ https://www.ncbi.nlm.nih.gov/pubmed/32075983 http://dx.doi.org/10.1038/s41467-020-14574-9 |
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author | Popovici, Jean Roesch, Camille Carias, Lenore L. Khim, Nimol Kim, Saorin Vantaux, Amelie Mueller, Ivo Chitnis, Chetan E. King, Christopher L. Witkowski, Benoit |
author_facet | Popovici, Jean Roesch, Camille Carias, Lenore L. Khim, Nimol Kim, Saorin Vantaux, Amelie Mueller, Ivo Chitnis, Chetan E. King, Christopher L. Witkowski, Benoit |
author_sort | Popovici, Jean |
collection | PubMed |
description | Antigenic variation, the capacity to produce a range of variable antigens, is a well-described strategy of Plasmodium and other parasites to evade host immunity. Here, we show that gene amplification is an additional evasion mechanism used by Plasmodium vivax to escape humoral immunity targeting PvDBP, the key ligand involved in reticulocyte invasion. PvDBP gene amplification leads to increased mRNA levels and protects P. vivax in vitro against invasion inhibitory human monoclonal antibodies targeting a conserved binding domain of DBP. Patient samples suggest that parasites with increased pvdbp copy number are able to infect individuals with naturally acquired antibodies highly blocking the binding of PvDBP to the Duffy receptor. These results show that gene copy number variation affect the parasite’s ability to evade anti-PvDBP humoral immunity. |
format | Online Article Text |
id | pubmed-7031336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-70313362020-03-04 Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity Popovici, Jean Roesch, Camille Carias, Lenore L. Khim, Nimol Kim, Saorin Vantaux, Amelie Mueller, Ivo Chitnis, Chetan E. King, Christopher L. Witkowski, Benoit Nat Commun Article Antigenic variation, the capacity to produce a range of variable antigens, is a well-described strategy of Plasmodium and other parasites to evade host immunity. Here, we show that gene amplification is an additional evasion mechanism used by Plasmodium vivax to escape humoral immunity targeting PvDBP, the key ligand involved in reticulocyte invasion. PvDBP gene amplification leads to increased mRNA levels and protects P. vivax in vitro against invasion inhibitory human monoclonal antibodies targeting a conserved binding domain of DBP. Patient samples suggest that parasites with increased pvdbp copy number are able to infect individuals with naturally acquired antibodies highly blocking the binding of PvDBP to the Duffy receptor. These results show that gene copy number variation affect the parasite’s ability to evade anti-PvDBP humoral immunity. Nature Publishing Group UK 2020-02-19 /pmc/articles/PMC7031336/ /pubmed/32075983 http://dx.doi.org/10.1038/s41467-020-14574-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Popovici, Jean Roesch, Camille Carias, Lenore L. Khim, Nimol Kim, Saorin Vantaux, Amelie Mueller, Ivo Chitnis, Chetan E. King, Christopher L. Witkowski, Benoit Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity |
title | Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity |
title_full | Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity |
title_fullStr | Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity |
title_full_unstemmed | Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity |
title_short | Amplification of Duffy binding protein-encoding gene allows Plasmodium vivax to evade host anti-DBP humoral immunity |
title_sort | amplification of duffy binding protein-encoding gene allows plasmodium vivax to evade host anti-dbp humoral immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031336/ https://www.ncbi.nlm.nih.gov/pubmed/32075983 http://dx.doi.org/10.1038/s41467-020-14574-9 |
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