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miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2
BACKGROUND: The aims of this study were to investigate the function and mechanism of miRNA‐98‐5p in papillary thyroid carcinoma. METHODS: Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to measure the expression of miRNA‐98‐5p in papillary thyroid carcinoma. Western blotting and...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031561/ https://www.ncbi.nlm.nih.gov/pubmed/31670857 http://dx.doi.org/10.1002/jcla.23044 |
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author | Qiu, Kai Xie, QingJi Jiang, Shan Lin, Ting |
author_facet | Qiu, Kai Xie, QingJi Jiang, Shan Lin, Ting |
author_sort | Qiu, Kai |
collection | PubMed |
description | BACKGROUND: The aims of this study were to investigate the function and mechanism of miRNA‐98‐5p in papillary thyroid carcinoma. METHODS: Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to measure the expression of miRNA‐98‐5p in papillary thyroid carcinoma. Western blotting and caspase‐3/9 activity levels, flow cytometric analysis, cell migration assays, DAPI assay, cell proliferation assay, and LDH activity levels were used in this study. RESULTS: In patient with papillary thyroid carcinoma, miRNA‐98‐5p was reduced, and HMGA2 was increased. Downregulation of miRNA‐98‐5p promoted the cell growth, inhibited apoptosis, and induced HMGA2 protein expression in papillary thyroid carcinoma cell via activation of HMGA2. Overexpression of miRNA‐98‐5p inhibited the cell growth, induced apoptosis, and suppressed HMGA2 protein expression in papillary thyroid carcinoma cell through the suppression of HMGA2. Si‐HMGA2 inhibited the effects of anti‐miRNA‐98‐5p on cell growth of papillary thyroid carcinoma. CONCLUSION: Therefore, these results suggested the regulation of HMGA2 suppresses proliferation of papillary thyroid carcinoma through miRNA‐98‐5p. |
format | Online Article Text |
id | pubmed-7031561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-70315612020-02-27 miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2 Qiu, Kai Xie, QingJi Jiang, Shan Lin, Ting J Clin Lab Anal Research Articles BACKGROUND: The aims of this study were to investigate the function and mechanism of miRNA‐98‐5p in papillary thyroid carcinoma. METHODS: Quantitative real‐time polymerase chain reaction (qRT‐PCR) was used to measure the expression of miRNA‐98‐5p in papillary thyroid carcinoma. Western blotting and caspase‐3/9 activity levels, flow cytometric analysis, cell migration assays, DAPI assay, cell proliferation assay, and LDH activity levels were used in this study. RESULTS: In patient with papillary thyroid carcinoma, miRNA‐98‐5p was reduced, and HMGA2 was increased. Downregulation of miRNA‐98‐5p promoted the cell growth, inhibited apoptosis, and induced HMGA2 protein expression in papillary thyroid carcinoma cell via activation of HMGA2. Overexpression of miRNA‐98‐5p inhibited the cell growth, induced apoptosis, and suppressed HMGA2 protein expression in papillary thyroid carcinoma cell through the suppression of HMGA2. Si‐HMGA2 inhibited the effects of anti‐miRNA‐98‐5p on cell growth of papillary thyroid carcinoma. CONCLUSION: Therefore, these results suggested the regulation of HMGA2 suppresses proliferation of papillary thyroid carcinoma through miRNA‐98‐5p. John Wiley and Sons Inc. 2019-10-31 /pmc/articles/PMC7031561/ /pubmed/31670857 http://dx.doi.org/10.1002/jcla.23044 Text en © 2019 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Qiu, Kai Xie, QingJi Jiang, Shan Lin, Ting miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2 |
title | miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2 |
title_full | miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2 |
title_fullStr | miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2 |
title_full_unstemmed | miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2 |
title_short | miR‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through Bax/Caspase‐3 by HMGA2 |
title_sort | mir‐98‐5p promotes apoptosis and inhibits migration and cell growth in papillary thyroid carcinoma through bax/caspase‐3 by hmga2 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7031561/ https://www.ncbi.nlm.nih.gov/pubmed/31670857 http://dx.doi.org/10.1002/jcla.23044 |
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